JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB313609

Human FUT8 ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Human FUT8 ELISA Kit is a Sandwich ELISA for the measurement of Human FUT8 in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

Alpha1-6FucT, Fucosyltransferase 8, GDP-fucose--glycoprotein fucosyltransferase, Glycoprotein 6-alpha-L-fucosyltransferase, FUT8

1 Images
Sandwich ELISA - Human FUT8 ELISA Kit (AB313609)
  • sELISA

Supplier Data

Sandwich ELISA - Human FUT8 ELISA Kit (AB313609)

Example of standard curve using ab313609. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, EDTA Plasma, Serum, Citrate plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.17 ng/mL

Range

0.17 - 40 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Complementary Products

Primary Antibodies

AB198741

Anti-FUT8 antibody

  • 0
  • 0
Proteins & Peptides

AB114702

Recombinant Human FUT8 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human FUT8 protein (GST tag N-Terminus) (AB114702)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Human FUT8 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human FUT8 in serum, plasma (collect plasma using Citrate or Heparin as an anticoagulant. EDTA is not recommended), and cell culture supernatants. This assay employs an antibody specific for human FUT8 coated on a 96-well plate.

This ELISA kit shows no cross-reactivity with the following cytokines tested: human Activin RIA, ASAHL, B4GalT1, BAI1, Brorin, C1qTNF4, CA14, CA4, CA6, CA8, Cadherin-6, Caspr2, CD27 Ligand, CD300a, CD300e, CD300f, CD4, CD5, CD69, CK18, CK19, CPB1, CRISP-2, DDR1, MIA, NTAL, NTB-A, OMgp, PEAR1, Podoplanin, PTH1R, Reg4, ROR1, Semaphorin 4G, Serpin A5, Serpin B6, Siglec-1, Sirtuin 2, Sirtuin 5.

style
left-column

Recovery

[ { "sample": "Serum", "range": "105 - 125 %", "average": "= 117.3" }, { "sample": "Plasma", "range": "97 - 118 %", "average": "= 108.1" }, { "sample": "Cell culture media", "range": "101 - 133 %", "average": "= 115.6" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab313609-1x96Tests", "assetComponentDetails": [ { "size":"1 x 200 µL", "name":"150X HRP-Streptavidin", "number":"AB313609-CMP03", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB313609-CMP04", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB313609-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human FUT8 Protein Standard", "number":"AB313609-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human FUT8 Antibody-coated ELISA Plate", "number":"AB313609-CMP08", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB313609-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB313609-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human FUT8 Detection Antibody", "number":"AB313609-CMP09", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FUT8 also known as alpha-(16)-fucosyltransferase represents an important enzyme responsible for transferring fucose from GDP-fucose to the innermost N-acetylglucosamine of N-glycans producing core fucosylation. The molecular mass of FUT8 is approximately 60 kDa. It expresses ubiquitously across various tissues with elevated levels seen in the liver lung and placenta. Researchers frequently study FUT8 due to its significance in glycosylation processes.
Biological function summary

FUT8 impacts numerous cellular activities by modulating glycan branching on glycoproteins. It functions independently and is not directly part of a larger protein complex. FUT8's activity influences cell-cell interactions signaling pathways and receptor functions. It plays an important part in maintaining cellular homeostasis and influences immune responses and cell growth as affected by glycosylation patterns on the cell surface and extracellular matrix.

Pathways

FUT8 is a critical component of the N-glycan biosynthesis pathway where it regulates the addition of fucose to glycans. This process affects protein folding and stability. The enzyme interacts with important glycoproteins like EGFR and integrins impacting signaling pathways such as the Wnt and TGF-beta pathways. Altered FUT8 activity can modify pathways and relationships with other proteins changing cellular responses.

FUT8 has connections to cancers and liver fibrosis due to its role in modifying glycans and subsequently influencing cellular communication and adhesion. Abnormal core fucosylation affects proteins like alpha-fetoprotein in liver cancer which might serve as a biomarker. Furthermore changes in FUT8 activity and expression could relate to epidermal growth factor receptor (EGFR) signaling disruptions promoting tumor proliferation. Scientists consider FUT8 a potential therapeutic target in these diseases.
style
left-column

Product protocols

style
left-column

Target data

Catalyzes the addition of fucose in alpha 1-6 linkage to the first GlcNAc residue, next to the peptide chains in N-glycans (PubMed : 17172260, PubMed : 29304374, PubMed : 36280670, PubMed : 9133635). Fucosylates the reducing GlcNAc residue in complex-type N-glycans attached on the fragment crystallizable (Fc) of IgGs. Fully converts Fc glycoforms containing one or two terminal GlcNAc moieties (G0-GlcNAc and G0) (PubMed : 36280670).
See full target information FUT8
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com