AB272771

Human FVIII ELISA kit (total FVIII antigen)

Be the first to review this product! Submit a review

(3 Publications)

Human FVIII ELISA kit (total FVIII antigen) is a Sandwich ELISA used to quantify Human FVIII (total FVIII antigen) with a sensitivity of 0.00024 IU/mL

- Colorimetric ELISA kit - 450 nm readout. Works on any standard plate reader
- Wide dynamic range – 0.0016 - 0.84 IU/mL

View Alternative Names

F8C, F8, Coagulation factor VIII, Antihemophilic factor, Procoagulant component, AHF

2 Images

ELISA - Human FVIII ELISA kit (total FVIII antigen) (AB272771)

ELISA

Supplier Data

ELISA - Human FVIII ELISA kit (total FVIII antigen) (AB272771)

A typical standard curve. Example only.

ELISA

Supplier Data

ELISA - Human FVIII ELISA kit (total FVIII antigen) (AB272771)

A typical standard curve. Example only.

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, EDTA Plasma

Reacts with

Human

Assay type

Quantitative

Range

0.0016 - 0.84 IU/mL

Assay Platform

Microplate

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

Product details

Human FVIII ELISA kit (total FVIII antigen) ab272771 is a sandwich ELISA kit for the quantitative measurement of FVIII total antigen with a sensitivity of 0.00024 IU/mL.

How the assay works

Human FVIII ELISA kit ab272771 uses a pair of antibodies each specific of different epitopes at the target of interest : the capture antibody is coated on the surface of the multi-well plate to facilitate the immobilization of the antigen. The other antibody is conjugated and facilitates the detection of the antigen.

Assay specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility.

Human FVIII ELISA kit (total FVIII antigen) ab272771 protocol summary

1. Add standard or sample to appropriate wells and shake plate at 300 rpm for 30 mins
2. Wash
3. Add primary antibody to each well and shake plate at 300 rpm for 30 mins
4. Wash
5. Add Streptavidin-HRP to each well and shake plate at 300 rpm for 30 mins
6. Wash
7. Add TMB substrate to each well and shake the plate for 2-10 min
8.Add Stop solution and read at 450nm.

How other researchers are using Human FVIII ELISA kit (total FVIII antigen) ab272771

Human FVIII ELISA kit (total FVIII antigen) ab272771 has been used to determine the efficiency of FVIII-secreting transgenic mesenchymal stem cells in correcting FVIII levels in an Hemophilia A model⁽¹⁾

References
⁽¹⁾C Hemsinlioglu et al. PMID: 37022209

Related and Recommended products

Human FVIII ELISA kit (total FVIII antigen) ab272771 is commonly used to study the coagulation cascade. Other kits used to study heamostasis are :
- Human Factor IX/PTC ELISA kit ab300307
- Factor VIIIa Activity Assay Kit (Fluorometric) ab204696
- Human Thrombin ELISA kit ab270210
- Human Tissue Factor ELISA kit ab220653
- Human Factor X ELISA Kit Human Factor X ELISA Kit
- Human Factor VII ELISA Kit ab190810

style

left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab272771-1x96Tests", "assetComponentDetails": [ { "size":"1 x 10 mL", "name":"TMB Substrate", "number":"AB272771-CMP06", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Streptavidin-HRP Secondary Reagent", "number":"AB272771-CMP05", "productcode":"" }, { "size":"1 x 50 mL", "name":"10X Wash Buffer", "number":"AB272771-CMP01", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Human FVIII Standard Lyophilized Vial", "number":"AB272771-CMP04", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Anti-Human FVIII Primary Antibody Lyophilized Vial", "number":"AB272771-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"ELISA Plate", "number":"AB272771-CMP07", "productcode":"" } ] } } }

style

left-column

Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Factor VIII also known as anti-hemophilic factor is a glycoprotein with a molecular mass of approximately 250 kDa. This protein circulates in the plasma mainly in the liver where hepatocytes produce it. Factor VIII remains inactive while circulating in the blood and exists within the factor VIII/von Willebrand factor complex. Upon activation it plays a significant role in the blood coagulation process.

Biological function summary

Factor VIII greatly contributes to hemostasis by serving as a cofactor for factor IXa forming the intrinsic tenase complex. This activation results in the conversion of factor X to factor Xa which is important for the common pathway of the coagulation cascade. By participating in the clotting process factor VIII ensures proper wound healing and prevention of blood loss.

Pathways

Factor VIII activity is pivotal within the coagulation cascade particularly the intrinsic and common pathways. Activation of the intrinsic pathway begins through interactions with factor IXa leading to efficient activation of factor X. This process closely relates factor VIII to proteins like factor IX and factor X ensuring proper clot formation and stabilization. Factor VIII products and assays are available to study and characterize its critical role in these pathways.

Factor VIII is closely associated with hemophilia A a genetic bleeding disorder resulting from deficiency or dysfunction of this protein. Patients with hemophilia A exhibit prolonged bleeding times due to impaired coagulation. The disorder links directly to von Willebrand factor as it protects factor VIII from degradation in circulation stabilizing its presence in the blood. Anti-factor VIII antibodies are important tools for diagnosing and studying factor VIII-related disorders.

style

left-column

Product protocols

style

left-column

Target data

Factor VIII, along with calcium and phospholipid, acts as a cofactor for F9/factor IXa when it converts F10/factor X to the activated form, factor Xa.

See full target information F8

style

left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cardiovascular medicine 11:1360364 PubMed38576426

2024

SARS-CoV-2 infection of endothelial cells, dependent on flow-induced ACE2 expression, drives hypercytokinemia in a vascularized microphysiological system.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher J Hatch,Sebastian D Piombo,Jennifer S Fang,Johannes S Gach,Makena L Ewald,William K Van Trigt,Brian G Coon,Jay M Tong,Donald N Forthal,Christopher C W Hughes

Turkish journal of haematology : official journal of Turkish Society of Haematology 40:118-124 PubMed37022209

2023

In Vitro FVIII-Encoding Transgenic Mesenchymal Stem Cells Maintain Successful Coagulation in FVIII-Deficient Plasma Mimicking Hemophilia A

Applications

Unspecified application

Species

Unspecified reactive species

Cansu Hemşinlioğlu,Elif Sibel Aslan,Cihan Taştan,Didem Çakırsoy,Raife Dilek Turan,Utku Seyis,Muhammer Elek,Gözde Sır Karakuş,Ömur Selin Günaydın,Selen Abanuz,Derya Dilek Kançağı,Bulut Yurtsever,Koray Yalçın,Murat Kasap,Ercüment Ovalı

Anticancer research 41:4761-4769 PubMed34593425

2021

Anti-inflammatory and Anti-thrombotic Efficacy of Targeted Ultrasound Microbubbles on LPS-induced HUVEC Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jindong Sun,Shijun Pan,Huamin Yu,Haiqiang Hu,Y U Sun,Zhijian Yang,Robert M Hoffman,Hong Yuan

View all publications

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human FVIII ELISA kit (total FVIII antigen)

ELISA - Human FVIII ELISA kit (total FVIII antigen) (AB272771)

ELISA - Human FVIII ELISA kit (total FVIII antigen) (AB272771)

Key facts

Detection method

Sample types

Reacts with

Assay type

Range

Assay Platform

Reactivity data

Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Complementary Products

Assay Kits

Secondary Antibodies

Assay Kits

Primary Antibodies

Product promise