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Human FVIII ELISA kit (total FVIII antigen) ab272771 is used to quantify Human FVIII with a sensitivity of 0.00024 IU/ml.

Sandwich ELISA Kit

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Images

ELISA - Human FVIII ELISA kit (total FVIII antigen) (AB272771), expandable thumbnail
  • ELISA - Human FVIII ELISA kit (total FVIII antigen) (AB272771), expandable thumbnail

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Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, EDTA Plasma

Assay type

Quantitative

Reactive species

Human

Range

0.0016 - 0.84 IU/mL

Reactivity data

Application

ELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Factor VIII, along with calcium and phospholipid, acts as a cofactor for F9/factor IXa when it converts F10/factor X to the activated form, factor Xa.

Alternative names

What's included?

1 x 96 Tests
Components
10X Wash Buffer
1 x 50 mL
Anti-Human FVIII Primary Antibody Lyophilized Vial
1 x 1 Vial
ELISA Plate
1 x 1 Unit
Human FVIII Standard Lyophilized Vial
1 x 1 Vial
Streptavidin-HRP Secondary Reagent
1 x 1 Vial
TMB Substrate
1 x 10 mL

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Human FVIII ELISA kit (total FVIII antigen) ab272771 is used to quantify Human FVIII with a sensitivity of 0.00024 IU/ml.

Sandwich ELISA Kit

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, EDTA Plasma

Assay type

Quantitative

Reactive species

Human

Range

0.0016 - 0.84 IU/mL

Assay Platform

Microplate

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

The sensitive quantitative measurement of total human Factor VIII antigen in plasma samples is easily performed with Human FVIII ELISA kit (total FVIII antigen) (ab272771).

The average normal plasma level of Factor VIII is defined as 1.0 IU/ml and the normal range is 0.4-1.8 IU/ml.

Hemophilia A patients are classified by the following Factor VIII levels:

0.05 - 0.25 IU/ml = mild

0.01 - 0.05 IU/ml = moderate

less than 0.01 IU/ml = severe

The assay measures total human Factor VIII in the 0.0016-0.84 IU/ml range. Samples giving human Factor VIII levels above 0.84 IU/ml should be diluted in blocking buffer before use. 1:8 and 1:16 dilutions for normal plasma, or 1:4 and 1:8 dilutions for Hemophiliac plasma, are suggested for best results.

Human Factor VIII will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, biotin labeled anti-human Factor VIII primary antibody binds to the captured protein. Excess primary antibody is washed away, and bound antibody is reacted with peroxidase conjugated streptavidin. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of human plasma. Color development is proportional to the concentration of Factor VIII in the samples. All reagents and standards are provided in these ELISA kits.

The Factor VIII level in the human plasma standard provided is calibrated against a secondary standard that is referenced to the WHO or ISTH International Standard.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Factor VIII also known as anti-hemophilic factor is a glycoprotein with a molecular mass of approximately 250 kDa. This protein circulates in the plasma mainly in the liver where hepatocytes produce it. Factor VIII remains inactive while circulating in the blood and exists within the factor VIII/von Willebrand factor complex. Upon activation it plays a significant role in the blood coagulation process.

Biological function summary

Factor VIII greatly contributes to hemostasis by serving as a cofactor for factor IXa forming the intrinsic tenase complex. This activation results in the conversion of factor X to factor Xa which is important for the common pathway of the coagulation cascade. By participating in the clotting process factor VIII ensures proper wound healing and prevention of blood loss.

Pathways

Factor VIII activity is pivotal within the coagulation cascade particularly the intrinsic and common pathways. Activation of the intrinsic pathway begins through interactions with factor IXa leading to efficient activation of factor X. This process closely relates factor VIII to proteins like factor IX and factor X ensuring proper clot formation and stabilization. Factor VIII products and assays are available to study and characterize its critical role in these pathways.

Associated diseases and disorders

Factor VIII is closely associated with hemophilia A a genetic bleeding disorder resulting from deficiency or dysfunction of this protein. Patients with hemophilia A exhibit prolonged bleeding times due to impaired coagulation. The disorder links directly to von Willebrand factor as it protects factor VIII from degradation in circulation stabilizing its presence in the blood. Anti-factor VIII antibodies are important tools for diagnosing and studying factor VIII-related disorders.

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2 product images

  • ELISA - Human FVIII ELISA kit (total FVIII antigen) (ab272771), expandable thumbnail

    ELISA - Human FVIII ELISA kit (total FVIII antigen) (ab272771)

    A typical standard curve. Example only.

  • ELISA - Human FVIII ELISA kit (total FVIII antigen) (ab272771), expandable thumbnail

    ELISA - Human FVIII ELISA kit (total FVIII antigen) (ab272771)

    A typical standard curve. Example only.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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