Human G-CSF R ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human G-CSF R in Human in Plasma, Cell culture supernatant, Serum samples.
Colorimetric
Plasma, Cell culture supernatant, Serum
Sandwich (quantitative)
Human
0.1 - 25 ng/mL
= 0.1 ng/mL
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Receptor for granulocyte colony-stimulating factor (CSF3), essential for granulocytic maturation. Plays a crucial role in the proliferation, differentiation and survival of cells along the neutrophilic lineage. In addition it may function in some adhesion or recognition events at the cell surface.
GCSFR, GCSFR, CSF3R, Granulocyte colony-stimulating factor receptor, G-CSF receptor, G-CSF-R
Human G-CSF R ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human G-CSF R in Human in Plasma, Cell culture supernatant, Serum samples.
Colorimetric
Plasma, Cell culture supernatant, Serum
Sandwich (quantitative)
Human
0.1 - 25 ng/mL
Microplate
= 0.1 ng/mL
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 10 |
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 12 |
Sample type | Average % | Range |
---|---|---|
Sample type Cell culture supernatant | Average % = 86.34 | Range 70 - 99 % |
Sample type Serum | Average % = 81.01 | Range 69 - 96 % |
Sample type Plasma | Average % = 77.54 | Range 68 - 92 % |
Blue Ice
-20°C
-20°C
-20°C
Human G-CSF R ELISA Kit is designed for the quantitative determination of G-CSF R in cell culture supernatants, plasma and serum samples.
This assay employs an antibody specific for human G-CSF R coated on a 96-well plate. Standards and samples are pipetted into the wells and G-CSF R present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-human G-CSF R antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of G-CSF R bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
This ELISA kit shows no cross-reactivity with the following cytokines tested: Human ACE-2, aFGF (FGF-1), Albumin, AMICA, Angiopoietin-4, BAFF, CA19-9, CD163, Clusterin, CRTAM, CXCL14, Cystatin C, Decorin, Dkk-3, DLL1, Fetuin A, FOLR1, Furin,GASP-1, GASP-2, HAI-2, IL-17 RB, IL-27, LAG-3, LDL R, Pepsinogen 1, RANK, RBP4, SOST, Syndecan-1, TACI, TFPI, Thrombospondin 1, TRAIL R2, TRANCE, Troponin I, uPA, VE-Cadherin, WISP-1.
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Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
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