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Human G-CSF R ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human G-CSF R in Human in Plasma, Cell culture supernatant, Serum samples.

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Images

Sandwich ELISA - Human G-CSF R ELISA Kit (AB267649), expandable thumbnail

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

0.1 - 25 ng/mL

Sensitivity

= 0.1 ng/mL

Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Receptor for granulocyte colony-stimulating factor (CSF3), essential for granulocytic maturation. Plays a crucial role in the proliferation, differentiation and survival of cells along the neutrophilic lineage. In addition it may function in some adhesion or recognition events at the cell surface.

Alternative names

What's included?

1 x 96 Tests
Components
20X Wash Buffer
1 x 25 mL
400X HRP-Streptavidin Concentrate
1 x 200 µL
5X Assay Diluent
1 x 15 mL
Anti-Human G-CSF R coated Microplate (12 x 8 wells)
1 x 1 Unit
Biotinylated Anti-Human G-CSF R Detection Antibody
2 x 1 Vial
Human G-CSF R Standard (Lyophilized)
2 x 1 Vial
Stop Solution
1 x 8 mL
TMB Substrate Solution
1 x 12 mL

Recommended products

Human G-CSF R ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human G-CSF R in Human in Plasma, Cell culture supernatant, Serum samples.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

0.1 - 25 ng/mL

Assay Platform

Microplate

Sensitivity

= 0.1 ng/mL

Precision

Intra assay

Sample

Overall

n

0

mean

-

SD

-

C.V.

< 10

Inter assay

Sample

Overall

n

0

mean

-

SD

-

C.V.

< 12

Recovery

Sample specific recovery

Sample type

Cell culture supernatant

Average %

= 86.34

Range

70 - 99 %

Sample type

Serum

Average %

= 81.01

Range

69 - 96 %

Sample type

Plasma

Average %

= 77.54

Range

68 - 92 %

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Human G-CSF R ELISA Kit is designed for the quantitative determination of G-CSF R in cell culture supernatants, plasma and serum samples.

This assay employs an antibody specific for human G-CSF R coated on a 96-well plate. Standards and samples are pipetted into the wells and G-CSF R present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-human G-CSF R antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of G-CSF R bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

This ELISA kit shows no cross-reactivity with the following cytokines tested: Human ACE-2, aFGF (FGF-1), Albumin, AMICA, Angiopoietin-4, BAFF, CA19-9, CD163, Clusterin, CRTAM, CXCL14, Cystatin C, Decorin, Dkk-3, DLL1, Fetuin A, FOLR1, Furin,GASP-1, GASP-2, HAI-2, IL-17 RB, IL-27, LAG-3, LDL R, Pepsinogen 1, RANK, RBP4, SOST, Syndecan-1, TACI, TFPI, Thrombospondin 1, TRAIL R2, TRANCE, Troponin I, uPA, VE-Cadherin, WISP-1.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Sandwich ELISA - Human G-CSF R ELISA Kit (ab267649), expandable thumbnail

    Sandwich ELISA - Human G-CSF R ELISA Kit (ab267649)

    Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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