Human G-CSF R ELISA Kit

Product details

Human G-CSF R ELISA Kit is designed for the quantitative determination of G-CSF R in cell culture supernatants, plasma and serum samples.

This assay employs an antibody specific for human G-CSF R coated on a 96-well plate. Standards and samples are pipetted into the wells and G-CSF R present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-human G-CSF R antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of G-CSF R bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

This ELISA kit shows no cross-reactivity with the following cytokines tested: Human ACE-2, aFGF (FGF-1), Albumin, AMICA, Angiopoietin-4, BAFF, CA19-9, CD163, Clusterin, CRTAM, CXCL14, Cystatin C, Decorin, Dkk-3, DLL1, Fetuin A, FOLR1, Furin,GASP-1, GASP-2, HAI-2, IL-17 RB, IL-27, LAG-3, LDL R, Pepsinogen 1, RANK, RBP4, SOST, Syndecan-1, TACI, TFPI, Thrombospondin 1, TRAIL R2, TRANCE, Troponin I, uPA, VE-Cadherin, WISP-1.