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AB315299

Human Galactosidase alpha ELISA Kit

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Human Galactosidase alpha ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Galactosidase alpha with a sensitivity of 54 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader

View Alternative Names

Alpha-galactosidase A, Alpha-D-galactosidase A, Alpha-D-galactoside galactohydrolase, Galactosylgalactosylglucosylceramidase GLA, Melibiase, GLA

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Sandwich ELISA - Human Galactosidase alpha ELISA Kit (AB315299)
  • sELISA

Supplier Data

Sandwich ELISA - Human Galactosidase alpha ELISA Kit (AB315299)

Example of human Galactosidase alpha standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Galactosidase alpha ELISA Kit (AB315299)
  • sELISA

Supplier Data

Sandwich ELISA - Human Galactosidase alpha ELISA Kit (AB315299)

Interpolated concentration of native Galactosidase alpha was measured in duplicate at different sample concentrations. Undiluted samples are as follows : human kidney extract 250 µg/mL and human brain stem extract 500 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Human Galactosidase alpha ELISA Kit (AB315299)
  • sELISA

Supplier Data

Sandwich ELISA - Human Galactosidase alpha ELISA Kit (AB315299)

Interpolated concentration of native Galactosidase alpha was measured in duplicate at different sample concentrations. Undiluted samples are as follows : MCF-7 extract 500 µg/mL, A431 extract 125 µg/mL, and 293T extract 100 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Human Galactosidase alpha ELISA Kit (AB315299)
  • sELISA

Supplier Data

Sandwich ELISA - Human Galactosidase alpha ELISA Kit (AB315299)

Representative image of Human Galactosidase alpha ELISA Kit (ab315299)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Human Galactosidase alpha Capture Antibody

- 10X Human Galactosidase alpha Detector Antibody

- Human Galactosidase alpha Lyophilised Purified Protein (2 vials)

- 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody diluent 5BI

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Tissue Extracts

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 54 pg/mL

Range

78.13 - 5000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Galactosidase alpha SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Galactosidase alpha protein in human serum, plasma, saliva and cell culture supernatant. Quantitate Human Galactosidase alpha with 24 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab315065) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.2" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 10.3" } ]
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Recovery

[ { "sample": "Cell Lysate", "range": "90 - 108 %", "average": "= 97" }, { "sample": "Tissue Extracts", "range": "80 - 110 %", "average": "= 97" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab315299-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB315299-CMP10", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB315299-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB315299-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB315299-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB315299-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB315299-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Galactosidase alpha Lyophilized Recombinant Protein", "number":"AB315299-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Galactosidase alpha Capture Antibody", "number":"AB315299-CMP01", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB315299-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Galactosidase alpha Detector Antibody", "number":"AB315299-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB315299-CMP11", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB315299-CMP12", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha-galactosidase also known as alpha-D-galactosidase or A-galactosidase is an enzyme that catalyzes the hydrolysis of terminal alpha-galactosyl moieties from glycolipids and glycoproteins. It has a molecular mass of approximately 50000 Daltons. This enzyme is expressed in various tissues including the liver heart and kidneys. It functions by efficiently removing alpha-galactose residues from target molecules a process important for maintaining cellular function.
Biological function summary

The role of alpha-galactosidase involves the breakdown of complex carbohydrates particularly those containing alpha-galactosidic bonds. It does not operate as part of a complex but rather functions independently to cleave these specific bonds in glycolipids and glycoproteins. This activity is critical for degrading langerin and other gangliosides preventing the accumulation of these molecules in the lysosomes. By continuously performing this function alpha-galactosidase maintains glycosphingolipid homeostasis ensuring efficient cellular metabolism.

Pathways

The involvement of alpha-galactosidase in metabolic and lysosomal catabolic pathways is significant. One critical pathway is the glycolipid metabolism pathway where it acts alongside related enzymes such as beta-galactosidase. It also participates in the lysosomal degradation pathway working in conjunction with other lysosomal enzymes to prevent substrate buildup. The interaction with enzymes like lysosomal hydrolases ensures the proper breakdown of complex molecules into simpler ones that cells can utilize or excrete.

Defects in the alpha-galactosidase enzyme lead to the lysosomal storage disorder known as Fabry disease. This genetic disorder causes the accumulation of globotriaosylceramide due to deficient alpha-galactosidase activity. Another disorder associated with malfunctioning alpha-galactosidase is cardiac complications due to tissue storage of glycolipids. In both cases the lack of enzyme activity disrupts cellular and tissue functions linking alpha-galactosidase to clinical symptoms seen in these disorders.
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Product protocols

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Target data

Catalyzes the hydrolysis of glycosphingolipids and participates in their degradation in the lysosome.
See full target information GLA
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websiteProtocolBooklet
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Product promise

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