Human Galectin-1 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Galectin-1 in Cell culture media, Heparin Plasma, Serum, EDTA Plasma samples.
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- Biomolecules 12:2022-GlcNAc-Mediated Regulation of Galectin Expression and Secretion in Human Promyelocytic HL-60 Cells Undergoing Neutrophilic Differentiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAdam McTague,Rada Tazhitdinova,Alexander V TimoshenkoPubMed 36551191
Key facts
- Detection method
Colorimetric
- Sample types
Cell culture media, Heparin Plasma, Serum, EDTA Plasma
- Assay type
Sandwich (quantitative)
- Reactive species
Mouse, Rat, Cow, Human
- Range
0.8 - 50 ng/mL
- Assay time
1h 30m
- Sensitivity
= 0.36 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Lectin that binds beta-galactoside and a wide array of complex carbohydrates. Plays a role in regulating apoptosis, cell proliferation and cell differentiation. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. Strong inducer of T-cell apoptosis.
Alternative names
Galectin-1, Gal-1, 14 kDa laminin-binding protein, 14 kDa lectin, Beta-galactoside-binding lectin L-14-I, Galaptin, HBL, HPL, Lactose-binding lectin 1, Lectin galactoside-binding soluble 1, Putative MAPK-activating protein PM12, S-Lac lectin 1, HLBP14, LGALS1
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Human Galectin-1 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Galectin-1 in Cell culture media, Heparin Plasma, Serum, EDTA Plasma samples.
Key facts
- Detection method
Colorimetric
- Sample types
Cell culture media, Heparin Plasma, Serum, EDTA Plasma
- Assay type
Sandwich (quantitative)
- Reactive species
Mouse, Rat, Cow, Human
- Range
0.8 - 50 ng/mL
- Assay time
1h 30m
- Assay Platform
Pre-coated microplate (12 x 8 well strips)
- Sensitivity
= 0.36 ng/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 3.2 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 4 | mean - | SD - | C.V. 5.6 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 97 | Range 88 - 102 % |
Sample type EDTA Plasma | Average % = 103 | Range 94 - 108 % |
Sample type Cell culture media | Average % = 107 | Range 98 - 114 % |
Sample type Heparin Plasma | Average % = 110 | Range 102 - 119 % |
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Human Galectin-1 ELISA Kit (ab260053) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Galectin-1 protein in cell culture media, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Galectin-1 with 0.36 ng/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Galectin-1, also known as 14 kDa lectin, galaptin, and lectin galactoside-binding soluble 1, is a β-galactoside binding lectin encoded by the gene LGALS1. Galectin-1 is a 134 amino acid non-glycosylated secreted protein that forms a non-covalently linked homodimer. Galectin-1 is expressed in a wide range of tissues and has various intra- and extra-cellular functions, where generally it is a modulator of immune responses. Galectin-1 may act as an autocrine negative growth factor that regulates cell proliferation.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Galectin-1 also referred to as GAL1 or galectin 1 is a protein with a molecular mass of approximately 14.5 kDa. It belongs to a group called the beta-galactoside-binding lectins and is typically characterized by its distinct carbohydrate-recognition domain. Galectin-1 is expressed in a variety of tissues including muscle epithelial cells the nervous system and immune cells. In particular high expression levels are found in the placenta and the thymus indicating a role in development and immune function.
- Biological function summary
This protein mediates a variety of cellular processes such as cell differentiation apoptosis and immune cell modulation. As a monomer galectin-1 binds specifically to beta-galactoside residues on the cell surface. It plays a significant role in regulation of cell signaling circuits affecting T-cell activation as well as macrophage function. The Galectin-1 protein does not commonly form complexes but can influence interactions of other proteins by binding to glycoproteins on the cell surface which can modulate signaling outcomes.
- Pathways
The protein is actively involved in pathways related to cell-cell interactions and immune modulation. It interacts with pathways like Ras signaling which is fundamental in cell growth proliferation and survival. Through these pathways galectin-1 is closely related to other proteins including Ras and Raf which are critical in transmitting signals from the cell surface to the DNA in the nucleus affecting gene expression involved in apoptosis and autophagy.
- Associated diseases and disorders
Galectin-1 association with cancer progression and autoimmune diseases reflects its role in immune system regulation. High galectin-1 levels have been recorded in various tumors where the protein promotes tumor progression by supporting angiogenesis and suppressing immune response against cancer cells. In autoimmune disorders such as multiple sclerosis modulation of Galectin-1 levels can alter disease progression and the immune system’s behavior. The protein interacts with other key regulators like cytokines emphasizing its importance in immune-inflammatory pathways and potential as a therapeutic target.
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4 product images
Sandwich ELISA - Human Galectin-1 ELISA Kit (ab260053)
Human Galectin-1 standard curve comparison.
Standard Curve comparison between human RANTES SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
Sandwich ELISA - Human Galectin-1 ELISA Kit (ab260053)
Example of human Galectin-1 standard curve in Sample Diluent NS.
The Galectin-1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Galectin-1 ELISA Kit (ab260053)
Interpolated concentrations of native Galectin-1 in human serum and plasma samples.
The concentrations of Galectin-1 were measured in duplicates, interpolated from the Galectin-1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (EDTA) 25%, and plasma (heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Galectin-1 concentration was determined to be 58 ng/mL in serum, 52.5 ng/mL in plasma (EDTA) and 72.8 ng/mL in plasma (heparin).
Sandwich ELISA - Human Galectin-1 ELISA Kit (ab260053)
Serum from eight individual healthy human donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Galectin-1 concentration was determined to be 99.2 ng/mL with a range of 4.3 –165.5 ng/mL.
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