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AB277447

Human Galectin 10 ELISA Kit

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Human Galectin 10 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Galectin 10 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

LGALS10, LGALS10A, CLC, Galectin-10, Gal-10, Charcot-Leyden crystal protein, Eosinophil lysophospholipase, Lysolecithin acylhydrolase

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ELISA - Human Galectin 10 ELISA Kit (AB277447)
  • ELISA

Supplier Data

ELISA - Human Galectin 10 ELISA Kit (AB277447)

These standard curves are for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.45 ng/mL

Range

0.41 - 100 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Galectin 10 ELISA Kit (ab277447) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Galectin 10 in serum (human Galectin 10 concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants.

This assay employs an antibody specific for Human Galectin 10 coated on a 96-well plate. Standards and samples are pipetted into the wells and Human Galectin 10 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human Galectin 10 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human Galectin 10 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": "72 - 83 %", "average": "= 77.23" }, { "sample": "Plasma", "range": "74 - 89 %", "average": "= 81.87" }, { "sample": "Cell culture media", "range": "74 - 93 %", "average": "= 84.75" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab277447-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB277447-CMP05", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB277447-CMP02", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB277447-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human Galectin 10 coated Microplate", "number":"AB277447-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB277447-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human Galection 10 Antibody", "number":"AB277447-CMP08", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB277447-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Galectin 10 standard protein (Lyophilized)", "number":"AB277447-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Galectin-10 also known as Charcot-Leyden crystal protein is part of the galectin family of proteins. It functions by binding to carbohydrates due to its unique lectin properties. With a molecular mass of approximately 16.5 kDa this protein is mainly expressed in eosinophils and basophils cells that are significant in the immune system. Galectin-10 is secreted by these cells and also stored in their granules suggesting its importance in immune responses.
Biological function summary

Galectin-10 influences cellular processes like apoptosis cell adhesion and immune cell migration contributing to immune regulation. It is not well known to form part of large complexes but its release has implications on cell signaling and tissue remodeling. The presence of galectin-10 in eosinophils suggests a role in inflammation given their involvement in allergic responses and asthma.

Pathways

Studies associate galectin-10 with the allergy response and eosinophil apoptosis pathways. This protein cooperates with other galectin family members like galectin-3 in modulating cellular responses. Signaling interactions of galectin-10 impact pathways involving cell survival and death highlighting its role in immune system dynamics.

Researchers link galectin-10 to conditions such as asthma and eosinophilic disorders. Eosinophil activity in these diseases often correlates with elevated galectin-10 levels in tissues and bloodstream. Connections exist between galectin-10 and other inflammation-related proteins like interleukins influencing disease progression and severity through their regulatory roles in immune responses.
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Product protocols

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Target data

Regulates immune responses through the recognition of cell-surface glycans. Essential for the anergy and suppressive function of CD25-positive regulatory T-cells (Treg).
See full target information CLC
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websiteProtocolBooklet
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