Name: Human Galectin-3 ELISA Kit (LGALS3)
SKU: ab269555
Rating: 5 (2 reviews)

Human Galectin-3 ELISA Kit (LGALS3) is a single-wash 90-min Simplestep used to quantify Human Galectin-3 (LGALS3) with a sensitivity of 13.3 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- KO validated for guaranteed specificity- Cited in over 5 citations

Images

ELISA - Human Galectin-3 ELISA Kit (LGALS3) (AB269555), expandable thumbnail

Publications

Key facts

Detection method: Colorimetric
Sample types: Cell culture media, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, Cell Lysate, EDTA Plasma
Assay type: Sandwich (quantitative)
Reactive species: Human
Range: 58.8 - 2000 pg/mL
Assay time: 1h 30m
Sensitivity: = 13.3 pg/mL

Reactivity data

Application	Reactivity	Dilution info	Notes
Application sELISA	Reactivity Reacts	Dilution info -	Notes -

Target data

See full target information LGALS3

Function

Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity). In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. Together with TRIM16, coordinates the recognition of membrane damage with mobilization of the core autophagy regulators ATG16L1 and BECN1 in response to damaged endomembranes.

Alternative names

MAC2, LGALS3, Galectin-3, Gal-3, 35 kDa lectin, Carbohydrate-binding protein 35, Galactose-specific lectin 3, Galactoside-binding protein, IgE-binding protein, L-31, Laminin-binding protein, Lectin L-29, Mac-2 antigen, CBP 35, GALBP

What's included?

1 x 96 Tests

Components

10X Human Galectin-3 (LGALS3) Capture Antibody

1 x 600 µL

10X Human Galectin-3 (LGALS3) Detector Antibody

1 x 600 µL

10X Wash Buffer PT (ab206977)

1 x 20 mL

5X Cell Extraction Buffer PTR (ab193970)

1 x 10 mL

Antibody Diluent 5BI

1 x 6 mL

Human Galectin-3 (LGALS3) Lyophilized Recombinant Protein

2 x 1 Vial

Plate Seals

1 x 1 Unit

Sample Diluent NS (ab193972)

2 x 50 mL

SimpleStep Pre-Coated 96-Well Microplate (ab206978)

1 x 1 Unit

Stop Solution

1 x 12 mL

TMB Development Solution

1 x 12 mL

Key facts

Detection method: Colorimetric
Sample types: Cell culture media, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, Cell Lysate, EDTA Plasma
Assay type: Sandwich (quantitative)
Reactive species: Human
Range: 58.8 - 2000 pg/mL
Assay time: 1h 30m
Assay Platform: Pre-coated microplate (12 x 8 well strips)
Sensitivity: = 13.3 pg/mL

Precision

Intra assay

Sample	n	C.V.
Sample Serum	n 8	C.V. 3.4

Inter assay

Sample	n	C.V.
Sample Serum	n 3	C.V. 1.6

Recovery

Sample specific recovery

Sample type	Average %	Range
Sample type Cell culture supernatant	Average % = 114	Range 109 - 120 %
Sample type Serum	Average % = 113	Range 107 - 120 %
Sample type EDTA Plasma	Average % = 113	Range 111 - 116 %
Sample type Cell culture media	Average % = 93	Range 91 - 95 %
Sample type Heparin Plasma	Average % = 109	Range 107 - 111 %
Sample type Cell Lysate	Average % = 109	Range 103 - 118 %
Sample type Citrate plasma	Average % = 110	Range 106 - 114 %

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Storage information: +4°C

Notes

Human Galectin-3 ELISA Kit (LGALS3) ab269555 is a rapid single-wash 90-min Sandwich ELISA to measure Human Galectin-3 (LGALS3)in cell culture media, cell culture supernatant, cell lysate, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 13.3 pg/mL.

How the assay works

Human Galectin-3 (LGALS3)SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Galectin-3 ELISA Kit (LGALS3) ab269555 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Galectin-3 (Gal-3, Galactoside-binding protein, Mac-2 antigen) is a member of the galectin family of carbohydrate-binding proteins. Galectin-3 is active intracellularly and extracellularly. It is widely expressed, including by fibroblasts and macrophages. Intracellularly, Galectin-3 can function in the nucleus as a pre-mRNA splicing factor and in the cytoplasm it has roles in endocytosis, exocytosis and apoptosis. Extracellularly, Galectin-3 is often pro-inflammatory and is involved in innate immunity. Galectin-3 can be monomeric or form higher order homo and hetero oligomers.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Galectin-3 also known as LGALS3 is a protein belonging to the galectin family which are beta-galactoside-binding lectins. This protein has a molecular weight of about 30 kDa. It shows expression in various tissues including the heart liver and gastrointestinal tract. Galectin-3 plays a mechanical role in cell-cell adhesion cell-matrix interactions and modulating immune responses. Researchers often use galectin-3 ELISA kits to quantify its presence in different biological samples.

Biological function summary

Galectin-3 participates in cell growth regulation apoptosis and immune system modulation. Galectin-3 does not typically integrate into larger molecular complexes but it interacts with a variety of cell surface and extracellular matrix molecules. Its function influences processes like cell differentiation and angiogenesis where it can act as a regulator leveraging its ability to bind glycans on cell surfaces and matrix components.

Pathways

Galectin-3 contributes to the regulation of pathways such as the MAPK signaling pathway and the Wnt signaling pathway. In the MAPK pathway it influences cell proliferation and survival. Galectin-3 interacts with proteins like ?-catenin in the Wnt pathway assisting in transcriptional regulation and cell fate determination. These pathways are vital for maintaining cellular homeostasis and responding to extracellular signals.

Associated diseases and disorders

Galectin-3 has a strong connection to cancer and fibrosis. Its role in cancer involves promoting tumor cell survival metastasis and angiogenesis where it interacts with proteins like integrins to facilitate tumor progression. In fibrosis galectin-3 contributes to fibrotic tissue formation especially in conditions like idiopathic pulmonary fibrosis and cardiac fibrosis often associated with proteins such as TGF-?. Understanding these interactions helps in developing therapeutic strategies targeting galectin-3 in these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

ELISA - Human Galectin-3 ELISA Kit (LGALS3) (ab269555)
Sandwich ELISA - Human Galectin-3 ELISA Kit (LGALS3) (ab269555)
Human Gelectin-3 standard curve comparison.
Standard Curve comparison between human Galectin-3 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
Sandwich ELISA - Human Galectin-3 ELISA Kit (LGALS3) (ab269555)
Human Gelectin-3 standard curve comparison.
Standard Curve comparison between the original Human Gelectin-3 SimpleStep ELISA kit (ab188394) and current Human Gelectin-3 SimpleStep ELISA kit (ab269555).
Sandwich ELISA - Human Galectin-3 ELISA Kit (LGALS3) (ab269555)
Example of human Galectin-3 standard curve in Sample Diluent NS.
The Galectin-3 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Galectin-3 ELISA Kit (LGALS3) (ab269555)
Interpolated concentrations of native Galectin-3 in human serum and plasma samples.
The concentrations of Galectin-3 were measured in duplicates, interpolated from the Galectin-3 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 6%, plasma (citrate) 6%, plasma (EDTA) 6% and plasma (heparin) 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Galectin-3 concentration was determined to be 34.5 ng/mL in neat serum, 30.3 ng/mL in neat plasma (citrate), 29.9 ng/mL in neat plasma (EDTA) and 14.8 ng/mL in neat plasma (heparin).
Sandwich ELISA - Human Galectin-3 ELISA Kit (LGALS3) (ab269555)
Interpolated concentrations of native Galectin-3 in human PBMC cell culture supernatant samples cultured for 36 hours.
The concentrations of Galectin-3 were measured in duplicates, interpolated from the Galectin-3 standard curves and corrected for sample dilution. Undiluted samples are as follows: PBMC supernatant 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Galectin-3 concentration was determined to be 17.2 ng/mL in neat PBMC supernatant.
Sandwich ELISA - Human Galectin-3 ELISA Kit (LGALS3) (ab269555)
Interpolated concentrations of native Galectin-3 in human MCF7 cell based on a 1.7 μg/mL extract load, Hap1 wild type and Galectin-3 knockout cells based on 200 µg/mL extract load.
The concentrations of Galectin-3 were measured in duplicate and interpolated from the Galectin-3 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Galectin-3 concentration was determined to be 1,855 pg/mL in MCF7 extract, 1,548 pg/mL in Hap1 wild type extract and undetectable in Hap1 Galectin-3 knockout extract.
Sandwich ELISA - Human Galectin-3 ELISA Kit (LGALS3) (ab269555)
Serum from ten individual healthy human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Galectin-3 concentration was determined to be 40.4 ng/mL with a range of 27.2 – 66.0 ng/mL.