Human Galectin-3 ELISA Kit (LGALS3) is a single-wash 90-min Simplestep used to quantify Human Galectin-3 (LGALS3) with a sensitivity of 13.3 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- KO validated for guaranteed specificity- Cited in over 5 citations
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- Experimental biology and medicine (Maywood, N.J.) 248:532-5402023Plasma Galectin-3 and H-FABP correlate with poor physical performance in patients with congestive heart failure.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFirdos Ahmad,Asima Karim,Javaidullah Khan,Rizwan QaisarPubMed 36803120
- Biomolecules 12:2022-GlcNAc-Mediated Regulation of Galectin Expression and Secretion in Human Promyelocytic HL-60 Cells Undergoing Neutrophilic Differentiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAdam McTague,Rada Tazhitdinova,Alexander V TimoshenkoPubMed 36551191
- Acta neuropathologica 144:843-8592022Galectin-3 is elevated in CSF and is associated with Aβ deposits and tau aggregates in brain tissue in Alzheimer's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAntonio Boza-Serrano,Agathe Vrillon,Karolina Minta,Agnes Paulus,Lluís Camprubí-Ferrer,Megg Garcia,Ulf Andreasson,Anna Antonell,Malin Wennström,Gunnar Gouras,Julien Dumurgier,Emmanuel Cognat,Laura Molina-Porcel,Mircea Balasa,Javier Vitorica,Raquel Sánchez-Valle,Claire Paquet,Jose Luis Venero,Kaj Blennow,Tomas DeierborgPubMed 35895141
- Molecular psychiatry 26:7803-78122021Angiogenic gene networks are dysregulated in opioid use disorder: evidence from multi-omics and imaging of postmortem human brain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEmily F Mendez,Haichao Wei,Ruifeng Hu,Laura Stertz,Gabriel R Fries,Xizi Wu,Katherine E Najera,Michael D Monterey,Christie M Lincoln,Joo-Won Kim,Karla Moriel,Thomas D Meyer,Sudhakar Selvaraj,Antonio L Teixeira,Zhongming Zhao,Junqian Xu,Jiaqian Wu,Consuelo Walss-BassPubMed 34385598
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture media, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, Cell Lysate, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 58.8 - 2000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 13.3 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity). In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. Together with TRIM16, coordinates the recognition of membrane damage with mobilization of the core autophagy regulators ATG16L1 and BECN1 in response to damaged endomembranes.
Alternative names
MAC2, LGALS3, Galectin-3, Gal-3, 35 kDa lectin, Carbohydrate-binding protein 35, Galactose-specific lectin 3, Galactoside-binding protein, IgE-binding protein, L-31, Laminin-binding protein, Lectin L-29, Mac-2 antigen, CBP 35, GALBP
What's included?
Recommended products
Human Galectin-3 ELISA Kit (LGALS3) is a single-wash 90-min Simplestep used to quantify Human Galectin-3 (LGALS3) with a sensitivity of 13.3 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- KO validated for guaranteed specificity- Cited in over 5 citations
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture media, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, Cell Lysate, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 58.8 - 2000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 13.3 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 8 | C.V. 3.4 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 3 | C.V. 1.6 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 114 | Range 109 - 120 % |
Sample type Serum | Average % = 113 | Range 107 - 120 % |
Sample type EDTA Plasma | Average % = 113 | Range 111 - 116 % |
Sample type Cell culture media | Average % = 93 | Range 91 - 95 % |
Sample type Heparin Plasma | Average % = 109 | Range 107 - 111 % |
Sample type Cell Lysate | Average % = 109 | Range 103 - 118 % |
Sample type Citrate plasma | Average % = 110 | Range 106 - 114 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human Galectin-3 ELISA Kit (LGALS3) (ab269555) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Galectin-3 (LGALS3) protein in cell lysate, serum, cell culture media, cit plasma, edta plasma, hep plasma, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Galectin-3 (LGALS3) with 13.3 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Galectin-3 (Gal-3, Galactoside-binding protein, Mac-2 antigen) is a member of the galectin family of carbohydrate-binding proteins. Galectin-3 is active intracellularly and extracellularly. It is widely expressed, including by fibroblasts and macrophages. Intracellularly, Galectin-3 can function in the nucleus as a pre-mRNA splicing factor and in the cytoplasm it has roles in endocytosis, exocytosis and apoptosis. Extracellularly, Galectin-3 is often pro-inflammatory and is involved in innate immunity. Galectin-3 can be monomeric or form higher order homo and hetero oligomers.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Galectin-3 also known as LGALS3 is a protein belonging to the galectin family which are beta-galactoside-binding lectins. This protein has a molecular weight of about 30 kDa. It shows expression in various tissues including the heart liver and gastrointestinal tract. Galectin-3 plays a mechanical role in cell-cell adhesion cell-matrix interactions and modulating immune responses. Researchers often use galectin-3 ELISA kits to quantify its presence in different biological samples.
- Biological function summary
Galectin-3 participates in cell growth regulation apoptosis and immune system modulation. Galectin-3 does not typically integrate into larger molecular complexes but it interacts with a variety of cell surface and extracellular matrix molecules. Its function influences processes like cell differentiation and angiogenesis where it can act as a regulator leveraging its ability to bind glycans on cell surfaces and matrix components.
- Pathways
Galectin-3 contributes to the regulation of pathways such as the MAPK signaling pathway and the Wnt signaling pathway. In the MAPK pathway it influences cell proliferation and survival. Galectin-3 interacts with proteins like β-catenin in the Wnt pathway assisting in transcriptional regulation and cell fate determination. These pathways are vital for maintaining cellular homeostasis and responding to extracellular signals.
- Associated diseases and disorders
Galectin-3 has a strong connection to cancer and fibrosis. Its role in cancer involves promoting tumor cell survival metastasis and angiogenesis where it interacts with proteins like integrins to facilitate tumor progression. In fibrosis galectin-3 contributes to fibrotic tissue formation especially in conditions like idiopathic pulmonary fibrosis and cardiac fibrosis often associated with proteins such as TGF-β. Understanding these interactions helps in developing therapeutic strategies targeting galectin-3 in these diseases.
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7 product images
Sandwich ELISA - Human Galectin-3 ELISA Kit (LGALS3) (ab269555)
Human Gelectin-3 standard curve comparison.
Standard Curve comparison between human Galectin-3 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
Sandwich ELISA - Human Galectin-3 ELISA Kit (LGALS3) (ab269555)
Human Gelectin-3 standard curve comparison.
Standard Curve comparison between the original Human Gelectin-3 SimpleStep ELISA kit (ab188394) and current Human Gelectin-3 SimpleStep ELISA kit (ab269555).
Sandwich ELISA - Human Galectin-3 ELISA Kit (LGALS3) (ab269555)
Example of human Galectin-3 standard curve in Sample Diluent NS.
The Galectin-3 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Galectin-3 ELISA Kit (LGALS3) (ab269555)
Interpolated concentrations of native Galectin-3 in human serum and plasma samples.
The concentrations of Galectin-3 were measured in duplicates, interpolated from the Galectin-3 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 6%, plasma (citrate) 6%, plasma (EDTA) 6% and plasma (heparin) 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Galectin-3 concentration was determined to be 34.5 ng/mL in neat serum, 30.3 ng/mL in neat plasma (citrate), 29.9 ng/mL in neat plasma (EDTA) and 14.8 ng/mL in neat plasma (heparin).
Sandwich ELISA - Human Galectin-3 ELISA Kit (LGALS3) (ab269555)
Interpolated concentrations of native Galectin-3 in human PBMC cell culture supernatant samples cultured for 36 hours.
The concentrations of Galectin-3 were measured in duplicates, interpolated from the Galectin-3 standard curves and corrected for sample dilution. Undiluted samples are as follows: PBMC supernatant 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Galectin-3 concentration was determined to be 17.2 ng/mL in neat PBMC supernatant.
Sandwich ELISA - Human Galectin-3 ELISA Kit (LGALS3) (ab269555)
Interpolated concentrations of native Galectin-3 in human MCF7 cell based on a 1.7 μg/mL extract load, Hap1 wild type and Galectin-3 knockout cells based on 200 µg/mL extract load.
The concentrations of Galectin-3 were measured in duplicate and interpolated from the Galectin-3 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Galectin-3 concentration was determined to be 1,855 pg/mL in MCF7 extract, 1,548 pg/mL in Hap1 wild type extract and undetectable in Hap1 Galectin-3 knockout extract.
Sandwich ELISA - Human Galectin-3 ELISA Kit (LGALS3) (ab269555)
Serum from ten individual healthy human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Galectin-3 concentration was determined to be 40.4 ng/mL with a range of 27.2 – 66.0 ng/mL.
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