Human Galectin-3 ELISA Kit (LGALS3) is a single-wash 90-min Simplestep used to quantify Human Galectin-3 (LGALS3) with a sensitivity of 13.3 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- KO validated for guaranteed specificity- Cited in over 5 citations
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity). In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. Together with TRIM16, coordinates the recognition of membrane damage with mobilization of the core autophagy regulators ATG16L1 and BECN1 in response to damaged endomembranes.
MAC2, LGALS3, Galectin-3, Gal-3, 35 kDa lectin, Carbohydrate-binding protein 35, Galactose-specific lectin 3, Galactoside-binding protein, IgE-binding protein, L-31, Laminin-binding protein, Lectin L-29, Mac-2 antigen, CBP 35, GALBP
Human Galectin-3 ELISA Kit (LGALS3) is a single-wash 90-min Simplestep used to quantify Human Galectin-3 (LGALS3) with a sensitivity of 13.3 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- KO validated for guaranteed specificity- Cited in over 5 citations
Sample | n | C.V. |
---|---|---|
Sample Serum | n 8 | C.V. 3.4 |
Sample | n | C.V. |
---|---|---|
Sample Serum | n 3 | C.V. 1.6 |
Sample type | Average % | Range |
---|---|---|
Sample type Cell culture supernatant | Average % = 114 | Range 109 - 120 % |
Sample type Serum | Average % = 113 | Range 107 - 120 % |
Sample type EDTA Plasma | Average % = 113 | Range 111 - 116 % |
Sample type Cell culture media | Average % = 93 | Range 91 - 95 % |
Sample type Heparin Plasma | Average % = 109 | Range 107 - 111 % |
Sample type Cell Lysate | Average % = 109 | Range 103 - 118 % |
Sample type Citrate plasma | Average % = 110 | Range 106 - 114 % |
Human Galectin-3 ELISA Kit (LGALS3) (ab269555) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Galectin-3 (LGALS3) protein in cell lysate, serum, cell culture media, cit plasma, edta plasma, hep plasma, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Galectin-3 (LGALS3) with 13.3 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Galectin-3 (Gal-3, Galactoside-binding protein, Mac-2 antigen) is a member of the galectin family of carbohydrate-binding proteins. Galectin-3 is active intracellularly and extracellularly. It is widely expressed, including by fibroblasts and macrophages. Intracellularly, Galectin-3 can function in the nucleus as a pre-mRNA splicing factor and in the cytoplasm it has roles in endocytosis, exocytosis and apoptosis. Extracellularly, Galectin-3 is often pro-inflammatory and is involved in innate immunity. Galectin-3 can be monomeric or form higher order homo and hetero oligomers.
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Galectin-3 also known as LGALS3 is a protein belonging to the galectin family which are beta-galactoside-binding lectins. This protein has a molecular weight of about 30 kDa. It shows expression in various tissues including the heart liver and gastrointestinal tract. Galectin-3 plays a mechanical role in cell-cell adhesion cell-matrix interactions and modulating immune responses. Researchers often use galectin-3 ELISA kits to quantify its presence in different biological samples.
Galectin-3 participates in cell growth regulation apoptosis and immune system modulation. Galectin-3 does not typically integrate into larger molecular complexes but it interacts with a variety of cell surface and extracellular matrix molecules. Its function influences processes like cell differentiation and angiogenesis where it can act as a regulator leveraging its ability to bind glycans on cell surfaces and matrix components.
Galectin-3 contributes to the regulation of pathways such as the MAPK signaling pathway and the Wnt signaling pathway. In the MAPK pathway it influences cell proliferation and survival. Galectin-3 interacts with proteins like β-catenin in the Wnt pathway assisting in transcriptional regulation and cell fate determination. These pathways are vital for maintaining cellular homeostasis and responding to extracellular signals.
Galectin-3 has a strong connection to cancer and fibrosis. Its role in cancer involves promoting tumor cell survival metastasis and angiogenesis where it interacts with proteins like integrins to facilitate tumor progression. In fibrosis galectin-3 contributes to fibrotic tissue formation especially in conditions like idiopathic pulmonary fibrosis and cardiac fibrosis often associated with proteins such as TGF-β. Understanding these interactions helps in developing therapeutic strategies targeting galectin-3 in these diseases.
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Human Gelectin-3 standard curve comparison.
Standard Curve comparison between human Galectin-3 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
Human Gelectin-3 standard curve comparison.
Standard Curve comparison between the original Human Gelectin-3 SimpleStep ELISA kit (ab188394) and current Human Gelectin-3 SimpleStep ELISA kit (ab269555).
Example of human Galectin-3 standard curve in Sample Diluent NS.
The Galectin-3 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Interpolated concentrations of native Galectin-3 in human serum and plasma samples.
The concentrations of Galectin-3 were measured in duplicates, interpolated from the Galectin-3 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 6%, plasma (citrate) 6%, plasma (EDTA) 6% and plasma (heparin) 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Galectin-3 concentration was determined to be 34.5 ng/mL in neat serum, 30.3 ng/mL in neat plasma (citrate), 29.9 ng/mL in neat plasma (EDTA) and 14.8 ng/mL in neat plasma (heparin).
Interpolated concentrations of native Galectin-3 in human PBMC cell culture supernatant samples cultured for 36 hours.
The concentrations of Galectin-3 were measured in duplicates, interpolated from the Galectin-3 standard curves and corrected for sample dilution. Undiluted samples are as follows: PBMC supernatant 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Galectin-3 concentration was determined to be 17.2 ng/mL in neat PBMC supernatant.
Interpolated concentrations of native Galectin-3 in human MCF7 cell based on a 1.7 μg/mL extract load, Hap1 wild type and Galectin-3 knockout cells based on 200 µg/mL extract load.
The concentrations of Galectin-3 were measured in duplicate and interpolated from the Galectin-3 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Galectin-3 concentration was determined to be 1,855 pg/mL in MCF7 extract, 1,548 pg/mL in Hap1 wild type extract and undetectable in Hap1 Galectin-3 knockout extract.
Serum from ten individual healthy human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Galectin-3 concentration was determined to be 40.4 ng/mL with a range of 27.2 – 66.0 ng/mL.
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