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AB213784

Human Galectin-3BP ELISA Kit

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(1 Publication)

Human Galectin-3BP ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Galectin-3BP in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

M2BP, LGALS3BP, Galectin-3-binding protein, Basement membrane autoantigen p105, Lectin galactoside-binding soluble 3-binding protein, Mac-2-binding protein, Tumor-associated antigen 90K, MAC2BP, Mac-2 BP

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Sandwich ELISA - Human Galectin-3BP ELISA Kit (AB213784)
  • sELISA

Supplier Data

Sandwich ELISA - Human Galectin-3BP ELISA Kit (AB213784)

Human Galectin-3BP ELISA Kit (ab213784) Standard Curve.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Heparin Plasma, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 10 pg/mL

Range

156 - 10000 pg/mL

Assay time

3h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

The Human Galectin-3BP Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213784) is designed for the quantitative measurement of Human Galectin-3BP in cell culture supernatants, serum and plasma (heparin).

The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Galectin-3BP has been pre-coated onto 96-well plates. Standards (NSO; V19-D585) and test samples are added to the wells, a biotinylated detection monoclonal antibody from mouse specific for Galectin-3BP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Galectin-3BP amount of sample captured in plate.

Galectin-3-binding protein is a protein that in humans is encoded by the LGALS3BP gene. The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell–cell and cell–matrix interactions. Using fluorescence in situ hybridization, the full length 90K cDNA has been localized to chromosome 17q25. The native protein binds specifically to a human macrophage associated lectin known as Mac-2 and also binds to galectin 1.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample 2", "replicates": 24, "mean": "1154 pg/mL", "standardDeviation": "84.24", "coefficientOfVariability": "= 7.3" }, { "reproducibilityType": "Intra", "sample": "Sample 2", "replicates": 16, "mean": "1090 pg/mL", "standardDeviation": "63.22", "coefficientOfVariability": "= 5.8" }, { "reproducibilityType": "Intra", "sample": "Sample 1", "replicates": 16, "mean": "312 pg/mL", "standardDeviation": "18.72", "coefficientOfVariability": "= 6" }, { "reproducibilityType": "Inter", "sample": "Sample 3", "replicates": 24, "mean": "3768 pg/mL", "standardDeviation": "271.29", "coefficientOfVariability": "= 7.2" }, { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 24, "mean": "326 pg/mL", "standardDeviation": "20.86", "coefficientOfVariability": "= 6.4" }, { "reproducibilityType": "Intra", "sample": "Sample 3", "replicates": 16, "mean": "4158 pg/mL", "standardDeviation": "282.74", "coefficientOfVariability": "= 6.8" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab213784-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"ABC Diluent Buffer", "number":"AB213784-CMP03", "productcode":"" }, { "size":"1 x 100 µL", "name":"Avidin-Biotin-Peroxidase Complex (ABC)", "number":"AB213784-CMP10", "productcode":"" }, { "size":"1 x 100 µL", "name":"Biotinylated anti- Human Galectin-3BP antibody", "number":"AB213784-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human Galectin-3BP coated Microplate (12 x 8 wells)", "number":"AB213784-CMP09", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Color Developing Agent", "number":"AB213784-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Antibody Diluent Buffer", "number":"AB213784-CMP08", "productcode":"" }, { "size":"1 x 30 mL", "name":"Sample Diluent Buffer", "number":"AB213784-CMP02", "productcode":"" }, { "size":"4 x 1 Unit", "name":"Adhesive Plate Seal", "number":"AB213784-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized recombinant Human Galectin-3BP standard", "number":"AB213784-CMP01", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Stop Solution", "number":"AB213784-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"Wash Buffer (25X)", "number":"AB213784-CMP11", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LGALS3BP also known as Galectin-3-binding protein (Mac-2 binding protein 90K) is a multifaceted glycoprotein with a mass of approximately 90 kDa. It typically interacts with galectins especially galectin-3 and is known for its adhesive properties. Researchers frequently observe it in various tissues including epithelial cells immune cells and in the extracellular matrix highlighting its involvement across different biological functions.
Biological function summary

The LGALS3BP glycoprotein modulates cell-cell and cell-matrix interactions. It does not operate alone; researchers have identified its presence in multiple complexes impacting several cellular processes such as immune modulation cell adhesion and migration. LGALS3BP can affect the immune response by influencing T-cell and natural killer cell activities which can alter the body's defense against pathogens and tumors.

Pathways

LGALS3BP significantly influences the signaling pathways involved with immune function and cellular adhesion. It plays an important role in the galectin pathway where it interacts with galectin-3 contributing to processes like cell growth and apoptosis. Additionally it affects the integrin-mediated signaling pathway enhancing cell adhesion and motility which is vital for cell migration and signaling dynamics. These interactions involve proteins such as integrins and other adhesion molecules that influence cellular communication and structural integrity.

LGALS3BP is strongly linked to cancer and inflammation-related conditions. Its overexpression is correlated with tumor progression and metastasis as seen in various cancers such as breast and colorectal cancer. LGALS3BP modulates its effects by interacting with galectin-3 influencing tumor-immune escape mechanisms and promoting tumor growth. Further its role in inflammatory disorders suggests it contributes to pathological processes through interactions with proteins that mediate inflammation and immune response.
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Product protocols

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Target data

Promotes integrin-mediated cell adhesion. May stimulate host defense against viruses and tumor cells.
See full target information LGALS3BP
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The journal of pathology. Clinical research 5:199-212 PubMed31136099

2019

Plasma proteome plus site-specific N-glycoprofiling for hepatobiliary carcinomas.

Applications

Unspecified application

Species

Unspecified reactive species

Ting-Tsung Chang,Ji-Hong Cheng,Hung-Wen Tsai,Kung-Chia Young,Sun-Yuan Hsieh,Cheng-Hsun Ho
View all publications
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