AB324406

Human Galectin-3BP (LGALS3BP) ELISA Kit- Extracellular

SimpleStep
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Human Galectin-3BP (LGALS3BP) ELISA Kit- Extracellular is a Sandwich ELISA for the measurement of Human Galectin-3BP (LGALS3BP) - Extracellular in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

M2BP, LGALS3BP, Galectin-3-binding protein, Basement membrane autoantigen p105, Lectin galactoside-binding soluble 3-binding protein, Mac-2-binding protein, Tumor-associated antigen 90K, MAC2BP, Mac-2 BP

8 Images

Sandwich ELISA - Human Galectin-3BP (LGALS3BP) ELISA Kit- Extracellular (AB324406)

sELISA

Lab

Sandwich ELISA - Human Galectin-3BP (LGALS3BP) ELISA Kit- Extracellular (AB324406)

Interpolated concentration of native Galectin-3BP was measured in duplicate at different sample concentrations. Undiluted samples are 1 : 4,000 serum, plasma (citrate), plasma (EDTA), and plasma (heparin). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Wash Buffer PT.

sELISA

Lab

Sandwich ELISA - Human Galectin-3BP (LGALS3BP) ELISA Kit- Extracellular (AB324406)

Interpolated concentration of native Galectin-3BP was measured in duplicate at different sample concentrations. Undiluted samples are 1 : 200 urine, saliva, and HepG2 cell culture supernatant. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Wash Buffer PT.

sELISA

Lab

Sandwich ELISA - Human Galectin-3BP (LGALS3BP) ELISA Kit- Extracellular (AB324406)

Interpolated concentrations of human Galectin-3BP in human serum in 96-well vs. 384-well plates. Interpolated concentration of native Galectin-3BP was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1 : 4,000 serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Wash Buffer PT.

sELISA

Lab

Sandwich ELISA - Human Galectin-3BP (LGALS3BP) ELISA Kit- Extracellular (AB324406)

Example of human Galectin-3BP standard curve in 1X Wash Buffer PT in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - Human Galectin-3BP (LGALS3BP) ELISA Kit- Extracellular (AB324406)

Interpolated concentration of native Galectin-3BP was measured in duplicate at different sample concentrations. Undiluted samples are 1 : 4,000 human and rhesus macaque serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Wash Buffer PT.

sELISA

Lab

Sandwich ELISA - Human Galectin-3BP (LGALS3BP) ELISA Kit- Extracellular (AB324406)

Specificity and interference testing of human Galectin-3BP with human Galectin-3. Recombinant human Galectin-3 was tested at 50 ng/mL for specificity, and at three different concentrations for interference in combination with 2.5 ng/mL of recombinant full-length mature human Galectin-3BP. The interpolated values, expressed as % of input (for specificity) or as % of human Galectin-3BP control (for interference) are plotted (mean +/- SD, n = 2).

sELISA

Lab

Sandwich ELISA - Human Galectin-3BP (LGALS3BP) ELISA Kit- Extracellular (AB324406)

Example of human Galectin-3BP standard curve in 1X Wash Buffer PT. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - Human Galectin-3BP (LGALS3BP) ELISA Kit- Extracellular (AB324406)

Standard curve comparison between Human Galectin-3BP SimpleStep ELISA kit and traditional ELISA kit from leading competitor. The SimpleStep ELISA kit shows increased sensitivity.

Key facts

Detection method

Colorimetric

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture media, Urine

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 5.184 pg/mL

Range

18.75 - 1200 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Galectin-3BP (LGALS3BP) ELISA Kit- Extracellular is a single-wash 90-min Simplestep used to quantify Human Galectin-3BP (LGALS3BP) with a sensitivity of 5.184pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colormetric sandwich ELISA - 450 nm readout- works on any standard plate reader.

- Different format for different needs: 10 x 96 plates for bulk orders and 384-well for higher throughput.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
- Also available in a fully validated 384-well format.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 9.8" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.3" } ]

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Recovery

[ { "sample": "Serum", "range": "95 - 112 %", "average": "= 104" }, { "sample": "Citrate plasma", "range": "88 - 100 %", "average": "= 96" }, { "sample": "EDTA Plasma", "range": "95 - 109 %", "average": "= 100" }, { "sample": "Heparin Plasma", "range": "96 - 109 %", "average": "= 100" }, { "sample": "Cell culture media", "range": "91 - 99 %", "average": "= 96" }, { "sample": "Urine", "range": "89 - 120 %", "average": "= 106" }, { "sample": "Saliva", "range": "84 - 109 %", "average": "= 93" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Promotes integrin-mediated cell adhesion. May stimulate host defense against viruses and tumor cells.

See full target information LGALS3BP

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websiteProtocolBooklet

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Product promise

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For full details, please see our Terms & Conditions

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