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AB213785

Human Galectin-4 ELISA Kit

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(1 Publication)

Human Galectin-4 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Galectin-4 in Human in Cell/Tissue Extracts, Cell Culture Media, Biofluids samples.

View Alternative Names

Galectin-4, Gal-4, Antigen NY-CO-27, L-36 lactose-binding protein, Lactose-binding lectin 4, L36LBP, LGALS4

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Sandwich ELISA - Human Galectin-4 ELISA Kit (AB213785)
  • sELISA

Supplier Data

Sandwich ELISA - Human Galectin-4 ELISA Kit (AB213785)

Human Galectin-4 ELISA Kit (ab213785) Standard Curve.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 10 pg/mL

Range

31.2 - 2000 pg/mL

Assay time

3h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

The Human Galectin-4 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213785) is designed for the quantitative measurement of Human Galectin-4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Galectin-4 has been pre-coated onto 96-well plates. Standards (Expression system for standard: E.coli; Immunogen sequence: A2-I323) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Galectin-4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Galectin-4 amount of sample captured in plate.

Galectin-4, also known as LGALS4 or GAL4, is a protein that in humans is encoded by the LGALS4 gene. It is mapped to 19q13.2. Galectins are a family of animal lectins defined by an affinity for beta galactoside-containing saccharides and by shared sequence elements. Galectin-4 is expressed as a 38-kD protein in the human colon adenocarcinoma T84 cell line. Galectin-4 is an S-type lectin that is strongly under expressed in colorectal cancer, and this gene plays a role in cell adhesion. It has been found that decreased Galectin-4 expression occurs early in colorectal carcinogenesis.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample 2", "replicates": 24, "mean": "1324 pg/mL", "standardDeviation": "108.56", "coefficientOfVariability": "= 8.2" }, { "reproducibilityType": "Intra", "sample": "Sample 3", "replicates": 16, "mean": "4066 pg/mL", "standardDeviation": "223.63", "coefficientOfVariability": "= 5.5" }, { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 24, "mean": "215 pg/mL", "standardDeviation": "12.04", "coefficientOfVariability": "= 5.6" }, { "reproducibilityType": "Inter", "sample": "Sample 3", "replicates": 24, "mean": "4394 pg/mL", "standardDeviation": "320.76", "coefficientOfVariability": "= 7.3" }, { "reproducibilityType": "Intra", "sample": "Sample 1", "replicates": 16, "mean": "209 pg/mL", "standardDeviation": "11.7", "coefficientOfVariability": "= 7.6" }, { "reproducibilityType": "Intra", "sample": "Sample 2", "replicates": 16, "mean": "1291 pg/mL", "standardDeviation": "98.11", "coefficientOfVariability": "= 7.6" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GAL4 also known as a transcription factor is an important protein in yeast. It plays a critical role in regulating the transcription of genes involved in galactose metabolism. The GAL4 protein has a mass of approximately 881 amino acids and is mainly expressed in the nucleus of yeast cells. Common parts of GAL4 include the GAL4 DBD (DNA-binding domain) and GAL4 AD (activation domain) which are critical for its function. Although similar sounding GAL4 should not be confused with galectin-4 or galectin 4 which are different proteins.
Biological function summary

The GAL4 transcription factor functions as a major regulator of galactose-responsive genes. It acts as part of a complex binding with its DNA target sites to initiate transcription of GAL genes like GAL1 GAL7 and GAL10. GAL4 protein recruits transcriptional machinery through its activation domain working alongside other factors to turn on specific gene expression in response to the presence of galactose. This capacity for gene regulation underlines its essential role in sugar metabolism in yeast.

Pathways

GAL4 is intimately involved in the GAL gene regulatory pathway that manages the cellular response to galactose. In this pathway proteins such as GAL80 inhibit GAL4 function under non-inducing conditions. However in the presence of galactose GAL3 binds and neutralizes GAL80 liberating GAL4 to activate gene expression. This system exemplifies a dynamic interaction in yeast allowing precise gene regulation in accordance with sugar availability.

While GAL4 is specifically a yeast protein and does not directly relate to human diseases its functional analogs and concepts have implications in genetic research and synthetic biology. For example GAL4 system is widely used in Drosophila and other model organisms to study gene expression patterns and related disorders. This system compared with mammalian transcription factors provides a foundational methodology for exploring genetic regulation mechanisms and potential implications in understanding metabolic disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Galectin that binds lactose and a related range of sugars. May be involved in the assembly of adherens junctions.
See full target information Galectin-4
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

BioMed research international 2020:8879758 PubMed33381596

2020

Evaluation of Serum and Gene Expression of Galectin-4, Interleukin-27, and Complement-7 in Hepatitis C Virus-Infected Egyptian Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Marwa S Abdel-Tawab,Hanan H Fouad,Dalia A Omran,Aml E Abdou,Shaimaa Mohamed Zaied,Alaa A Mohamed
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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