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AB100523

Human Galectin 7 ELISA Kit (GAL7)

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Human Galectin 7 ELISA Kit (GAL7) is a Sandwich (quantitative) ELISA for the measurement of Human Galectin 7 (GAL7) in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

PIG1, LGALS7B, LGALS7, Galectin-7, Gal-7, HKL-14, PI7, p53-induced gene 1 protein

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Sandwich ELISA - Human Galectin 7 ELISA Kit (GAL7) (AB100523)
  • sELISA

Supplier Data

Sandwich ELISA - Human Galectin 7 ELISA Kit (GAL7) (AB100523)

Representative Standard Curve using ab100523.

Sandwich ELISA - Human Galectin 7 ELISA Kit (GAL7) (AB100523)
  • sELISA

Supplier Data

Sandwich ELISA - Human Galectin 7 ELISA Kit (GAL7) (AB100523)

Representative Standard Curve using ab100523.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 20 pg/mL

Range

13.72 - 10000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Galectin 7 (GAL7) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Galectin 7 in serum, plasma, and cell culture supernatants.

This assay employs an antibody specific for Human Galectin 7 coated on a 96-well plate. Standards and samples are pipetted into the wells and Galectin 7 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Galectin 7 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Galectin 7 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Optimization may be required with urine samples

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Recovery

[ { "sample": "Cell culture supernatant", "range": "87 - 105 %", "average": "= 96.55" }, { "sample": "Serum", "range": "95 - 115 %", "average": "= 107.1" }, { "sample": "Plasma", "range": "70 - 125 %", "average": "= 91.82" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100523-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100523-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100523-CMP05", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100523-CMP04", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100523-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Galectin 7 Microplate (12 x 8 well strips)", "number":"AB100523-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human Galectin 7", "number":"AB100523-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Galectin 7 Standard Human (recombinant) (lyophilized)", "number":"AB100523-CMP08", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB100523-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100523-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Galectin 7 also known as Gal-7 is a member of the galectin family of beta-galactoside-binding proteins. It has a molecular mass of approximately 15 kDa. Structurally Galectin 7 has a single carbohydrate recognition domain (CRD) which allows it to bind glycoconjugates. Galectin 7 expresses in various tissues with high expression levels in the epidermis and in stratified squamous epithelia.
Biological function summary

Galectin 7 plays a role in cell-cell adhesion apoptosis regulation and immune response modulation. It plays a role in the cell's apoptosis machinery and interacts with the cytochrome c release pathway. This protein does not typically form a part of large complexes functioning individually in most biological processes. It can influence keratinocyte migration indicating involvement in wound healing processes.

Pathways

Galectin 7 participates in the apoptosis and cell adhesion pathways. It works alongside proteins such as Bcl-2 and Bax to regulate programmed cell death by promoting apoptosis. Additionally it interacts with matrix metalloproteinases influencing the remodelling of extracellular matrix a critical component of tissue repair and immune response.

Galectin 7 is associated with cancer and psoriasis. In cancer it can act as a pro-apoptotic factor and modulates tumor progression often in concert with p53 a well-known tumor suppressor protein. In psoriasis aberrant expression of Galectin 7 correlates with impaired epidermal function and inflammation suggesting it may alter the disease course by influencing local immune responses and keratinocyte behavior.
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Product protocols

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Target data

Could be involved in cell-cell and/or cell-matrix interactions necessary for normal growth control. Pro-apoptotic protein that functions intracellularly upstream of JNK activation and cytochrome c release.
See full target information LGALS7
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websiteProtocolBooklet
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