AB273161

Human galectin-9 ELISA kit (Gal-9)

Name: Human galectin-9 ELISA kit (Gal-9) (ab273161) | Abcam
Brand: Abcam Limited
SKU: AB273161
Price: 780 USD
Availability: InStock

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Human galectin-9 ELISA kit (Gal-9) is a Sandwich (quantitative) ELISA for the measurement of Human galectin-9 (Gal-9) in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

Galectin-9, Gal-9, Ecalectin, Tumor antigen HOM-HD-21, LGALS9

1 Images

ELISA - Human galectin-9 ELISA kit (Gal-9) (AB273161)

ELISA

Supplier Data

ELISA - Human galectin-9 ELISA kit (Gal-9) (AB273161)

Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

Example of Human Galectin-9 ELISA kit (Gal-9) standard curve.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 8 pg/mL

Range

15.6 - 500 pg/mL

Assay time

3h 45m

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Galectin-9 ELISA kit (Gal-9) (ab273161) is a solid phase sandwich ELISA for the in-vitro qualitative and quantitative determination of Galectin-9 in supernatants, buffered solutions or serum and plasma samples. This assay will recognise both natural and recombinant human Galectin-9.

A capture antibody highly specific for Galectin-9 has been coated to the wells of the microtiter strip plate provided during manufacture. Binding of Galectin-9 in samples and known standards to the capture antibodies is completed and then any excess unbound analyte is removed.

During the next incubation period the binding of the biotinylated anti-Galectin-9 secondary antibody to the analyte occurs. Any excess unbound secondary antibody is then removed.

The HRP conjugate solution is then added to every well including the zero wells, following incubation excess conjugate is removed by careful washing.

A chromogen substrate is added to the wells resulting in the progressive development of a blue coloured complex with the conjugate. The colour development is then stopped by the addition of acid turning the resultant final product yellow. The intensity of the produced coloured complex is directly proportional to the concentration of Galectin-9 present in the samples and standards.

The absorbance of the colour complex is then measured and the generated OD values for each standard are plotted against expected concentration forming a standard curve. This standard curve can then be used to accurately determine the concentration of Galectin-9 in any sample tested.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 0, "mean": "345.43 pg/mL", "standardDeviation": "15.45", "coefficientOfVariability": "4.5" }, { "reproducibilityType": "Inter", "sample": "Sample 2", "replicates": 0, "mean": "189.62 pg/mL", "standardDeviation": "25.4", "coefficientOfVariability": "13.4" }, { "reproducibilityType": "Inter", "sample": "Sample 3", "replicates": 0, "mean": "461.78 pg/mL", "standardDeviation": "11.51", "coefficientOfVariability": "2.5" }, { "reproducibilityType": "Inter", "sample": "Sample 4", "replicates": 0, "mean": "249.55 pg/mL", "standardDeviation": "13.28", "coefficientOfVariability": "5.3" }, { "reproducibilityType": "Inter", "sample": "Sample 5", "replicates": 0, "mean": "589.98 pg/mL", "standardDeviation": "8.15", "coefficientOfVariability": "1.4" }, { "reproducibilityType": "Inter", "sample": "Sample 6", "replicates": 0, "mean": "296.4 pg/mL", "standardDeviation": "21.38", "coefficientOfVariability": "7.2" }, { "reproducibilityType": "Intra", "sample": "Various", "replicates": 18, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.2" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab273161-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"HRP Diluent", "number":"AB273161-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Gal-9 Standard", "number":"AB273161-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Gal-9 Coated Microwell strips", "number":"AB273161-CMP03", "productcode":"" }, { "size":"1 x 400 µL", "name":"Biotinylated Antibody Gal-9", "number":"AB273161-CMP02", "productcode":"" }, { "size":"1 x 11 mL", "name":"TMB Substrate", "number":"AB273161-CMP06", "productcode":"" }, { "size":"1 x 10 mL", "name":"Wash Buffer", "number":"AB273161-CMP08", "productcode":"" }, { "size":"1 x 7 mL", "name":"Biotinylated Antibody diluent", "number":"AB273161-CMP01", "productcode":"" }, { "size":"2 x 5 µL", "name":"Streptavidin-HRP", "number":"AB273161-CMP07", "productcode":"" }, { "size":"2 x 1 Unit", "name":"Plastic Plate Cover", "number":"AB273161-CMP11", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Reagent", "number":"AB273161-CMP10", "productcode":"" }, { "size":"1 x 15 mL", "name":"Standard diluent Buffer", "number":"AB273161-CMP09", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Galectin-9 also known as Gal-9 or LGALS9 is a β-galactoside-binding lectin with a molecular mass of around 40 kDa. It shows expression in various tissues including the thymus spleen and liver. The galectin-9 protein is involved in cell-cell and cell-matrix interactions where it binds to glycoproteins and glycolipids. Researchers commonly use methods like Galectin-9 ELISA to measure its levels in different biological samples.

Biological function summary

Galectins like galectin-9 play essential roles in regulating diverse immune responses. Galectin-9 acts within the immune system by modulating inflammation and apoptosis. It acts as both a pro-apoptotic factor for T cells and a protein mediator in innate immune functions. Galectin-9 proteins can form complexes with other lectins and cell surface receptors contributing to cellular signaling processes.

Pathways

Galectin-9 is integral to the immune system's functional networks. It participates in the T-cell regulation pathways and the innate immunity pathways. In these pathways it interacts with other proteins like TIM-3 which mediates T-cell apoptosis and contributes to immune tolerance. These interaction networks play important roles in maintaining the body's immune homeostasis.

Galectin-9 has associations with autoimmune diseases and cancer. Its regulatory effects on T cells connect it to autoimmune conditions where abnormal T-cell activation occurs. In cancer the alteration in galectin-9 expression levels affects tumor immunity. The interaction of galectin-9 with other proteins like TIM-3 is important in cancer progression making it a target of interest for therapeutic interventions.

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Product protocols

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Target data

Binds galactosides (PubMed : 18005988). Has high affinity for the Forssman pentasaccharide (PubMed : 18005988). Ligand for HAVCR2/TIM3 (PubMed : 16286920). Binding to HAVCR2 induces T-helper type 1 lymphocyte (Th1) death (PubMed : 16286920). Also stimulates bactericidal activity in infected macrophages by causing macrophage activation and IL1B secretion which restricts intracellular bacterial growth (By similarity). Ligand for P4HB; the interaction retains P4HB at the cell surface of Th2 T-helper cells, increasing disulfide reductase activity at the plasma membrane, altering the plasma membrane redox state and enhancing cell migration (PubMed : 21670307). Ligand for CD44; the interaction enhances binding of SMAD3 to the FOXP3 promoter, leading to up-regulation of FOXP3 expression and increased induced regulatory T (iTreg) cell stability and suppressive function (By similarity). Promotes ability of mesenchymal stromal cells to suppress T-cell proliferation (PubMed : 23817958). Expands regulatory T-cells and induces cytotoxic T-cell apoptosis following virus infection (PubMed : 20209097). Activates ERK1/2 phosphorylation inducing cytokine (IL-6, IL-8, IL-12) and chemokine (CCL2) production in mast and dendritic cells (PubMed : 16116184, PubMed : 24465902). Inhibits degranulation and induces apoptosis of mast cells (PubMed : 24465902). Induces maturation and migration of dendritic cells (PubMed : 16116184, PubMed : 25754930). Inhibits natural killer (NK) cell function (PubMed : 23408620). Can transform NK cell phenotype from peripheral to decidual during pregnancy (PubMed : 25578313). Astrocyte derived galectin-9 enhances microglial TNF production (By similarity). May play a role in thymocyte-epithelial interactions relevant to the biology of the thymus. May provide the molecular basis for urate flux across cell membranes, allowing urate that is formed during purine metabolism to efflux from cells and serving as an electrogenic transporter that plays an important role in renal and gastrointestinal urate excretion (By similarity). Highly selective to the anion urate (By similarity).. Isoform 2. Acts as an eosinophil chemoattractant (PubMed : 9642261). It also inhibits angiogenesis (PubMed : 24333696). Suppresses IFNG production by natural killer cells (By similarity).

See full target information LGALS9

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

In vivo (Athens, Greece) 37:2577-2584 PubMed37905655

2023

Evaluation of the Relationship Between miRNA-22-3p and Gal-9 Levels in Glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Zerrin Barut,Fatma Tuba Akdeniz

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human galectin-9 ELISA kit (Gal-9)

ELISA - Human galectin-9 ELISA kit (Gal-9) (AB273161)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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