JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB213786

Human Galectin-9 / Gal-9 ELISA Kit

Be the first to review this product! Submit a review

|

(1 Publication)

Human Galectin-9 / Gal-9 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Galectin-9 / Gal-9 in Human in Cell/Tissue Extracts, Cell Culture Media, Biofluids samples.

View Alternative Names

Galectin-9, Gal-9, Ecalectin, Tumor antigen HOM-HD-21, LGALS9

1 Images
Sandwich ELISA - Human Galectin-9 / Gal-9 ELISA Kit (AB213786)
  • sELISA

Supplier Data

Sandwich ELISA - Human Galectin-9 / Gal-9 ELISA Kit (AB213786)

Human Galectin-9 / Gal-9 ELISA Kit (ab213786) Standard Curve

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 10 pg/mL

Range

93.8 - 6000 pg/mL

Assay time

3h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

Product details

The Human Galectin-9 / Gal-9 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213786) is designed for the quantitative detection of Human Galectin-9 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A polyclonal antibody from goat specific for Galectin-9 has been precoated onto 96-well plates. Standards (Expression system for standard: E.coli; Immunogen sequence: M1-T355) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Galectin-9 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Galectin-9 amount of sample captured in plate.

Galectin-9, also called HUAT or LGALS9A is a protein that in humans is encoded by the LGALS9 gene. This gene is mapped to 17q11.2. The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. The protein encoded by this gene is an S-type lectin. It is overexpressed in Hodgkin's disease tissue and might participate in the interaction between the H and RS cells with their surrounding cells and might thus, play a role in the pathogenesis of this disease and/or its associated immunodeficiency. The protein has N- and C- terminal carbohydrate-binding domains connected by a link peptide.

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 24, "mean": "177 pg/mL", "standardDeviation": "9.55", "coefficientOfVariability": "= 5.4" }, { "reproducibilityType": "Intra", "sample": "Sample 3", "replicates": 16, "mean": "3580 pg/mL", "standardDeviation": "218.38", "coefficientOfVariability": "= 6.1" }, { "reproducibilityType": "Inter", "sample": "Sample 2", "replicates": 24, "mean": "792 pg/mL", "standardDeviation": "66.52", "coefficientOfVariability": "= 8.4" }, { "reproducibilityType": "Inter", "sample": "Sample 3", "replicates": 24, "mean": "3920 pg/mL", "standardDeviation": "274.4", "coefficientOfVariability": "= 7" }, { "reproducibilityType": "Intra", "sample": "Sample 1", "replicates": 16, "mean": "168 pg/mL", "standardDeviation": "7.9", "coefficientOfVariability": "= 4.7" }, { "reproducibilityType": "Intra", "sample": "Sample 2", "replicates": 16, "mean": "731 pg/mL", "standardDeviation": "51.9", "coefficientOfVariability": "= 7.1" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab213786-1x96Tests", "assetComponentDetails": [ { "size":"1 x 100 µL", "name":"Avidin-Biotin-Peroxidase Complex (ABC)", "number":"AB213786-CMP10", "productcode":"" }, { "size":"1 x 100 µL", "name":"Biotinylated anti-Human Galectin-9 antibody", "number":"AB213786-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human Galectin-9 coated Microplate (12 x 8 wells)", "number":"AB213786-CMP09", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Color Developing Agent", "number":"AB213786-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"Antibody Diluent Buffer", "number":"AB213786-CMP08", "productcode":"" }, { "size":"1 x 30 mL", "name":"Sample Diluent Buffer", "number":"AB213786-CMP03", "productcode":"" }, { "size":"4 x 1 Unit", "name":"Adhesive Plate Seal", "number":"AB213786-CMP07", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Stop Solution", "number":"AB213786-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized recombinant Human Galectin-9 standard", "number":"AB213786-CMP01", "productcode":"" }, { "size":"1 x 20 mL", "name":"Wash Buffer (25X)", "number":"AB213786-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"ABC Diluent Buffer", "number":"AB213786-CMP04", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Galectin-9 also known as Gal-9 or LGALS9 is a β-galactoside-binding lectin with a molecular mass of around 40 kDa. It shows expression in various tissues including the thymus spleen and liver. The galectin-9 protein is involved in cell-cell and cell-matrix interactions where it binds to glycoproteins and glycolipids. Researchers commonly use methods like Galectin-9 ELISA to measure its levels in different biological samples.
Biological function summary

Galectins like galectin-9 play essential roles in regulating diverse immune responses. Galectin-9 acts within the immune system by modulating inflammation and apoptosis. It acts as both a pro-apoptotic factor for T cells and a protein mediator in innate immune functions. Galectin-9 proteins can form complexes with other lectins and cell surface receptors contributing to cellular signaling processes.

Pathways

Galectin-9 is integral to the immune system's functional networks. It participates in the T-cell regulation pathways and the innate immunity pathways. In these pathways it interacts with other proteins like TIM-3 which mediates T-cell apoptosis and contributes to immune tolerance. These interaction networks play important roles in maintaining the body's immune homeostasis.

Galectin-9 has associations with autoimmune diseases and cancer. Its regulatory effects on T cells connect it to autoimmune conditions where abnormal T-cell activation occurs. In cancer the alteration in galectin-9 expression levels affects tumor immunity. The interaction of galectin-9 with other proteins like TIM-3 is important in cancer progression making it a target of interest for therapeutic interventions.
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Binds galactosides (PubMed : 18005988). Has high affinity for the Forssman pentasaccharide (PubMed : 18005988). Ligand for HAVCR2/TIM3 (PubMed : 16286920). Binding to HAVCR2 induces T-helper type 1 lymphocyte (Th1) death (PubMed : 16286920). Also stimulates bactericidal activity in infected macrophages by causing macrophage activation and IL1B secretion which restricts intracellular bacterial growth (By similarity). Ligand for P4HB; the interaction retains P4HB at the cell surface of Th2 T-helper cells, increasing disulfide reductase activity at the plasma membrane, altering the plasma membrane redox state and enhancing cell migration (PubMed : 21670307). Ligand for CD44; the interaction enhances binding of SMAD3 to the FOXP3 promoter, leading to up-regulation of FOXP3 expression and increased induced regulatory T (iTreg) cell stability and suppressive function (By similarity). Promotes ability of mesenchymal stromal cells to suppress T-cell proliferation (PubMed : 23817958). Expands regulatory T-cells and induces cytotoxic T-cell apoptosis following virus infection (PubMed : 20209097). Activates ERK1/2 phosphorylation inducing cytokine (IL-6, IL-8, IL-12) and chemokine (CCL2) production in mast and dendritic cells (PubMed : 16116184, PubMed : 24465902). Inhibits degranulation and induces apoptosis of mast cells (PubMed : 24465902). Induces maturation and migration of dendritic cells (PubMed : 16116184, PubMed : 25754930). Inhibits natural killer (NK) cell function (PubMed : 23408620). Can transform NK cell phenotype from peripheral to decidual during pregnancy (PubMed : 25578313). Astrocyte derived galectin-9 enhances microglial TNF production (By similarity). May play a role in thymocyte-epithelial interactions relevant to the biology of the thymus. May provide the molecular basis for urate flux across cell membranes, allowing urate that is formed during purine metabolism to efflux from cells and serving as an electrogenic transporter that plays an important role in renal and gastrointestinal urate excretion (By similarity). Highly selective to the anion urate (By similarity).. Isoform 2. Acts as an eosinophil chemoattractant (PubMed : 9642261). It also inhibits angiogenesis (PubMed : 24333696). Suppresses IFNG production by natural killer cells (By similarity).
See full target information LGALS9
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Biomolecules 12: PubMed36551191

2022

-GlcNAc-Mediated Regulation of Galectin Expression and Secretion in Human Promyelocytic HL-60 Cells Undergoing Neutrophilic Differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Adam McTague,Rada Tazhitdinova,Alexander V Timoshenko
View all publications
style
left-column
style
left-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
style
left-column
websiteProtocolBooklet
en
style
right-column
style
bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com