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AB313610

Human GALNT3 ELISA Kit

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Human GALNT3 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human GALNT3 in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

Polypeptide N-acetylgalactosaminyltransferase 3, Polypeptide GalNAc transferase 3, Protein-UDP acetylgalactosaminyltransferase 3, UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 3, GalNAc-T3, pp-GaNTase 3, GALNT3

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Sandwich ELISA - Human GALNT3 ELISA Kit (AB313610)
  • sELISA

Supplier Data

Sandwich ELISA - Human GALNT3 ELISA Kit (AB313610)

Example of standard curve using ab313610. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.39 ng/mL

Range

0.39 - 100 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human GALNT3 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human GALNT3 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human GALNT3 coated on a 96-well plate.

This ELISA kit shows no cross-reactivity with the following cytokines tested: human Adenosine Deaminase/ADA, AIF, AKR1C4 , ASAH2, BCL-2, BID, Calreticulin, Calreticulin-2, CD314 (NKG2D), CD39L3/ENTPD3, CD51, CD99-L2, CDC25B, Cerberus 1, CHST2, Cochlin, CRELD2, DC-SIGNR/CD299, eNOS, ENPP2/Autotaxin, FABP4, Fc epsilon RI, FGF R5, GALNT2, GIF, GPR111, beta-Glucuronidase/GUSB, Inhibin A, LILRB4/CD85k/ILT3, Neuroglycan C/CSPG5, NKp46/NCR1, Neuronal Pentraxin R/NPTXR, ROR2, SCCA2, Siglec2/CD22, SIRP alpha/CD172a, SorCS1, Trypsin 1/PRSS1, Trypsin 3/PRSS3.

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Recovery

[ { "sample": "Serum", "range": "71 - 128 %", "average": "= 95.76" }, { "sample": "Plasma", "range": "75 - 127 %", "average": "= 94.59" }, { "sample": "Cell culture media", "range": "83 - 131 %", "average": "= 106.5" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GALNT3 also known as polypeptide N-acetylgalactosaminyltransferase 3 is an enzyme that plays an important role in the O-glycosylation process. It transfers N-acetylgalactosamine (GalNAc) to serine and threonine residues of proteins. This enzyme belongs to the glycosyltransferase family and has a molecular mass of approximately 70 kDa. GALNT3 is mainly expressed in tissues such as liver kidney small intestine and bone.
Biological function summary

GALNT3 is responsible for initiating O-linked glycosylation of proteins affecting their stability function and localization. It acts alone and not as part of a larger protein complex. Through its activity GALNT3 modulates various proteins involved in cellular functions such as signal transduction and cell-cell interaction. This glycosylation process helps in the correct folding of proteins and protecting them from proteolytic degradation.

Pathways

The role of GALNT3 places it prominently in the glycosylation pathway where it influences the modification of proteins involved in the signaling cascade. One important pathway involving GALNT3 is the regulation of mineralization controlled by fibroblast growth factor 23 (FGF23). GALNT3 glycosylates and activates FGF23 highlighting its relationship to other proteins within the signaling axis including the fibroblast growth factor receptors (FGFRs).

Alterations in GALNT3 are associated with familial tumoral calcinosis a disorder characterized by abnormal calcium and phosphate metabolism. Mutations in GALNT3 disrupt its function leading to improper glycosylation and degradation of FGF23 resulting in elevated levels of phosphate. The connection between GALNT3 and FGF23 is central in understanding the pathogenesis of this condition as well as other metabolic bone diseases related to phosphate homeostasis.
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Product protocols

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Target data

Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor (PubMed : 16638743, PubMed : 31932717, PubMed : 8663203, PubMed : 9295285). Has activity toward HIV envelope glycoprotein gp120, EA2, MUC2, MUC1A and MUC5AC (PubMed : 8663203, PubMed : 9295285). Probably glycosylates fibronectin in vivo (PubMed : 9295285). Glycosylates FGF23 (PubMed : 16638743, PubMed : 31932717).
See full target information Polypeptide N-acetylgalactosaminyltransferase 3
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