AB224884

Human gamma-synuclein ELISA Kit

Recombinant
SimpleStep
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Human gamma-synuclein ELISA Kit is a single-wash 90-min Simplestep used to quantify Human gamma-synuclein with a sensitivity of 5.15 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

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Sandwich ELISA - Human gamma-synuclein ELISA Kit (AB224884)

sELISA

Supplier Data

Sandwich ELISA - Human gamma-synuclein ELISA Kit (AB224884)

Example of human gamma-synuclein standard curve in Sample Diluent NS.

The gamma-synuclein standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human gamma-synuclein ELISA Kit (AB224884)

Urine from pooled normal human and four individual bladder cancer patients (male : n=3; female : n=1) human donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean gamma-synuclein concentration was determined to be 569.88 pg/mL with a range of 40.46 – 2021.56 pg/ml. The native concentration of gamma-synuclein was below the level of detection in urine from healthy human donors.

sELISA

Supplier Data

Sandwich ELISA - Human gamma-synuclein ELISA Kit (AB224884)

Interpolated concentrations of native gamma-synuclein in human serum and plasma samples.

The concentrations of gamma-synuclein were measured in duplicate, interpolated from the gamma-synuclein standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 25%, plasma (citrate) 25%, plasma (EDTA) 25%, plasma (heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean gamma-synuclein concentration was determined to be 126.89 pg/mL in serum, 114.40 pg/mL in plasma (citrate), 78.63 pg/mL in plasma (EDTA) and 173.43 pg/ml in plasma (heparin).

sELISA

Supplier Data

Sandwich ELISA - Human gamma-synuclein ELISA Kit (AB224884)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean gamma-synuclein concentration was determined to be 180.4 pg/mL with a range of 32.92 - 451.67 pg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human gamma-synuclein ELISA Kit (AB224884)

Interpolated concentrations of native gamma-synuclein in human urine (from a bladder cancer patient) and cell culture extract based on a 5 μg/mL load.

The concentrations of gamma-synuclein were measured in duplicate and interpolated from the gamma-synuclein standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean gamma-synuclein concentration was determined to be 2193.46 pg/mL in bladder cancer urine, 316.85 pg/ml in HT-29 cell extract.

sELISA

Supplier Data

Sandwich ELISA - Human gamma-synuclein ELISA Kit (AB224884)

Example of human gamma-synuclein standard curve in Sample Diluent PTR.

The gamma-synuclein standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Urine, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 5.15 pg/mL

Range

7.81 - 500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human gamma-synuclein ELISA Kit (ab224884) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of gamma-synuclein protein in cit plasma, edta plasma, hep plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human gamma-synuclein with 5.15 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Human gamma-synuclein plays a role in neurofilament network integrity. May be involved in modulating axonal architecture during development and in the adult. In vitro, increases the susceptibility of neurofilament-H to calcium-dependent proteases (By similarity). May also function in modulating the keratin network in skin. Activates the MAPK and Elk-1 signal transduction pathway.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "14" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.1" } ]

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Recovery

[ { "sample": "Serum", "range": "90 - 105 %", "average": "= 100" }, { "sample": "EDTA Plasma", "range": "66 - 77 %", "average": "= 73" }, { "sample": "Urine", "range": "100 - 114 %", "average": "= 107" }, { "sample": "Heparin Plasma", "range": "83 - 92 %", "average": "= 89" }, { "sample": "Citrate plasma", "range": "75 - 84 %", "average": "= 79" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Gamma synuclein sometimes referred to as SNCG or gamma-synuclein is a protein with a molecular weight of approximately 13 kDa. It is a member of the synuclein family which also includes alpha and beta synucleins. This protein is highly expressed in the nervous system particularly in the peripheral nervous system and certain regions of the central nervous system. Outside the nervous system gamma synuclein can be found in the retina reproductive tissues and breast tissue among others.

Biological function summary

Gamma synuclein plays a role in modulating neuronal plasticity and may participate in synaptic regulation. It is not known to be a part of a larger protein complex but it interacts with various other proteins influencing neuron differentiation. Although its precise biological functions are not fully characterized studies suggest that gamma synuclein could impact cellular processes like cytoskeletal dynamics and membrane trafficking.

Pathways

The protein gamma synuclein is involved in cellular stress response and neurodegeneration pathways. It is closely related to proteins like alpha synuclein with which it shares structural and functional features. Pathways involving these proteins can influence several processes such as axonal transport and neurotransmitter release. Dysregulation in these pathways often leads to disruptions in cellular homeostasis.

The involvement of gamma synuclein is most notably seen in breast cancer and neurodegenerative diseases such as Alzheimer's disease. Researchers have observed that this protein is overexpressed in various tumors where it might facilitate cancer progression and metastasis. In neurodegeneration interaction with alpha synuclein and tau protein could play a role in the pathogenesis where protein aggregation is a characteristic feature. Understanding its role in these diseases helps in charting possible therapeutic avenues.

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Product protocols

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Target data

Plays a role in neurofilament network integrity. May be involved in modulating axonal architecture during development and in the adult. In vitro, increases the susceptibility of neurofilament-H to calcium-dependent proteases (By similarity). May also function in modulating the keratin network in skin. Activates the MAPK and Elk-1 signal transduction pathway (By similarity).

See full target information SNCG

Alternative Names

BCSG1, PERSYN, PRSN, SNCG, Gamma-synuclein, Breast cancer-specific gene 1 protein, Persyn, Synoretin, SR

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human gamma-synuclein ELISA Kit

Sandwich ELISA - Human gamma-synuclein ELISA Kit (AB224884)

Sandwich ELISA - Human gamma-synuclein ELISA Kit (AB224884)

Sandwich ELISA - Human gamma-synuclein ELISA Kit (AB224884)

Sandwich ELISA - Human gamma-synuclein ELISA Kit (AB224884)

Sandwich ELISA - Human gamma-synuclein ELISA Kit (AB224884)

Sandwich ELISA - Human gamma-synuclein ELISA Kit (AB224884)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Assay Kits

Primary Antibodies

Proteins & Peptides

Assay Kits

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Alternative Names

Product promise