Skip to main content

Human GAP43 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human GAP43 in Tissue Extracts samples.

Be the first to review this product! Submit a review

Images

Sandwich ELISA - Human GAP43 ELISA Kit (AB282867), expandable thumbnail
  • Sandwich ELISA - Human GAP43 ELISA Kit (AB282867), expandable thumbnail

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

15.6 - 1000 pg/mL

Assay time

1h 30m

Sensitivity

= 5.9 pg/mL

Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info
-
Notes

-

Target data

Function

This protein is associated with nerve growth. It is a major component of the motile 'growth cones' that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction.

Alternative names

What's included?

1 x 96 Tests
Components
10X Human GAP43 Capture Antibody
1 x 600 µL
10X Human GAP43 Detector Antibody
1 x 600 µL
10X Wash Buffer PT (ab206977)
1 x 20 mL
5X Cell Extraction Buffer PTR (ab193970)
1 x 10 mL
Antibody Diluent 4BR
1 x 6 mL
Human GAP43 Lyophilized Recombinant Protein
2 x 1 Vial
Plate Seals
1 x 1 Unit
Sample Diluent NS (ab193972)
1 x 12 mL
SimpleStep Pre-Coated 96-Well Microplate (ab206978)
1 x 1 Unit
Stop Solution
1 x 12 mL
TMB Development Solution
1 x 12 mL

Recommended products

Human GAP43 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human GAP43 in Tissue Extracts samples.

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

15.6 - 1000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Sensitivity

= 5.9 pg/mL

Precision

Intra assay

Sample

Extract

n
8
mean
-
SD
-
C.V.

8.8

Inter assay

Sample

Extract

n
4
mean
-
SD
-
C.V.

9.8

Recovery

Sample specific recovery

Sample type

Tissue Extracts

Average %

= 90

Range

81 - 105 %

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Human GAP43 ELISA kit (ab282867) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human GAP43 protein in human tissue extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human GAP43 with 13.8 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Axonal membrane protein Growth associated protein (GAP43), also known as Neuromodulin, Neural phosphoprotein B-50, and pp46, is a protein encoded by the gene GAP43. GAP43 is a nervous system-specific protein that is bound to the neuronal membrane of by dual palmitoylation sequences. The protein is associated with nerve growth and is a major component of the motile "growth cones" that form the tips of elongating axons. GAP43 is phosphorylate by a protein kinase C, which is correlate with some forms of synaptic plasticity.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

GAP43 commonly called Growth Associated Protein 43 or neuronal marker is a protein with a molecular weight of approximately 25-50 kDa. Researchers identify it by various names including GAP-43 GAP43 anticuerpo or GAP43 neuronal marker. This protein exhibits a specific presence in the nervous system especially in axons of neurons. It has significant expression during neural development and regeneration indicating its role in growth and repair.

Biological function summary

GAP43 plays an important role in neurite outgrowth and synaptic plasticity. It functions as an important mediator of signal transduction in neurons. GAP43 does not act alone but integrates into a network of signaling pathways that guide axonal elongation and branching. It works closely with cytoskeletal elements influencing the structural dynamics needed for effective neuronal response and adaptation.

Pathways

GAP43 is vital in the regulation of the protein kinase C pathway and the cAMP pathway both essential for neuronal growth and differentiation. It closely interacts with proteins such as calmodulin which binds to GAP43 and impacts its function in those pathways. These interactions highlight its role in the modulation of neuronal growth and synaptic plasticity making it essential for neurons' response to environmental signals.

Associated diseases and disorders

GAP43 associates with neurodegenerative diseases and cognitive disorders particularly Alzheimer's disease and schizophrenia. Changes in GAP43 expression levels correlate with the severity and progression of these conditions. Additionally GAP43 connects to other proteins implicated in such diseases like tau in Alzheimer's suggesting a multifaceted involvement in disease mechanisms. This makes it a target of interest for therapeutic research as modulating GAP43 activity might alter disease progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Sandwich ELISA - Human GAP43 ELISA Kit (ab282867), expandable thumbnail

    Sandwich ELISA - Human GAP43 ELISA Kit (ab282867)

    Interpolated concentrations of native GAP43 in human brain based on a 0.31 μg/mL extract load.

    The concentrations of GAP43 were measured in duplicate and interpolated from the GAP43 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

  • Sandwich ELISA - Human GAP43 ELISA Kit (ab282867), expandable thumbnail

    Sandwich ELISA - Human GAP43 ELISA Kit (ab282867)

    Example of human GAP43 standard curve in Sample Diluent 1X Cell Extraction Buffer PTR.

    The GAP43 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com