Human GAP43 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human GAP43 in Tissue Extracts samples.
Colorimetric
Tissue Extracts
Sandwich (quantitative)
Human
15.6 - 1000 pg/mL
1h 30m
= 5.9 pg/mL
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
This protein is associated with nerve growth. It is a major component of the motile 'growth cones' that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction.
Neuromodulin, Axonal membrane protein GAP-43, Growth-associated protein 43, Neural phosphoprotein B-50, pp46, GAP43
Human GAP43 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human GAP43 in Tissue Extracts samples.
Colorimetric
Tissue Extracts
Sandwich (quantitative)
Human
15.6 - 1000 pg/mL
1h 30m
Pre-coated microplate (12 x 8 well strips)
= 5.9 pg/mL
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Extract | n 8 | mean - | SD - | C.V. 8.8 |
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Extract | n 4 | mean - | SD - | C.V. 9.8 |
Sample type | Average % | Range |
---|---|---|
Sample type Tissue Extracts | Average % = 90 | Range 81 - 105 % |
Blue Ice
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Human GAP43 ELISA kit (ab282867) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human GAP43 protein in human tissue extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human GAP43 with 13.8 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Axonal membrane protein Growth associated protein (GAP43), also known as Neuromodulin, Neural phosphoprotein B-50, and pp46, is a protein encoded by the gene GAP43. GAP43 is a nervous system-specific protein that is bound to the neuronal membrane of by dual palmitoylation sequences. The protein is associated with nerve growth and is a major component of the motile "growth cones" that form the tips of elongating axons. GAP43 is phosphorylate by a protein kinase C, which is correlate with some forms of synaptic plasticity.
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GAP43 commonly called Growth Associated Protein 43 or neuronal marker is a protein with a molecular weight of approximately 25-50 kDa. Researchers identify it by various names including GAP-43 GAP43 anticuerpo or GAP43 neuronal marker. This protein exhibits a specific presence in the nervous system especially in axons of neurons. It has significant expression during neural development and regeneration indicating its role in growth and repair.
GAP43 plays an important role in neurite outgrowth and synaptic plasticity. It functions as an important mediator of signal transduction in neurons. GAP43 does not act alone but integrates into a network of signaling pathways that guide axonal elongation and branching. It works closely with cytoskeletal elements influencing the structural dynamics needed for effective neuronal response and adaptation.
GAP43 is vital in the regulation of the protein kinase C pathway and the cAMP pathway both essential for neuronal growth and differentiation. It closely interacts with proteins such as calmodulin which binds to GAP43 and impacts its function in those pathways. These interactions highlight its role in the modulation of neuronal growth and synaptic plasticity making it essential for neurons' response to environmental signals.
GAP43 associates with neurodegenerative diseases and cognitive disorders particularly Alzheimer's disease and schizophrenia. Changes in GAP43 expression levels correlate with the severity and progression of these conditions. Additionally GAP43 connects to other proteins implicated in such diseases like tau in Alzheimer's suggesting a multifaceted involvement in disease mechanisms. This makes it a target of interest for therapeutic research as modulating GAP43 activity might alter disease progression.
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Interpolated concentrations of native GAP43 in human brain based on a 0.31 μg/mL extract load.
The concentrations of GAP43 were measured in duplicate and interpolated from the GAP43 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Example of human GAP43 standard curve in Sample Diluent 1X Cell Extraction Buffer PTR.
The GAP43 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed
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