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AB267608

Human Gas 1 ELISA Kit

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Human Gas 1 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Gas 1 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

Growth arrest-specific protein 1, GAS-1, GAS1

1 Images
Sandwich ELISA - Human Gas 1 ELISA Kit (AB267608)
  • sELISA

Unknown

Sandwich ELISA - Human Gas 1 ELISA Kit (AB267608)

This standard curve is for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 45 pg/mL

Range

46.88 - 3000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Gas 1 ELISA Kit is designed for the quantitative determination of Gas 1 in cell culture supernatants, plasma and serum samples.

This assay employs an antibody specific for human Gas 1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Gas 1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Gas 1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Gas 1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
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Recovery

[ { "sample": "Serum", "range": "84 - 116 %", "average": "= 99.34" }, { "sample": "Plasma", "range": "76 - 106 %", "average": "= 91.77" }, { "sample": "Cell culture media", "range": "82 - 118 %", "average": "= 98.45" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab267608-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB267608-CMP02", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB267608-CMP06", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB267608-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human Gas 1 coated Microplate (12 x 8 wells)", "number":"AB267608-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human Gas 1 Detection Antibody", "number":"AB267608-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB267608-CMP04", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB267608-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Gas 1 Standard (Lyophilized)", "number":"AB267608-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The growth arrest-specific 1 protein or Gas1 plays an inhibitory role in cell cycle progression by interacting with various growth factors and receptors. Gas1 often termed as Gas-1 is a 38 kDa protein. It expresses prominently in quiescent cells particularly in the nervous and embryonic tissues. This expression helps control the proliferation of cells which is critical for maintaining normal cellular functions and structure.
Biological function summary

Gas1 acts as a negative regulator of cell proliferation particularly during developmental processes. It is not part of a complex but serves as a standalone inhibitor that influences cell growth. The protein interacts with components like Hedgehog signaling which leads to its involvement in developmental signaling pathways. Gas1's role in growth arrest demonstrates its importance in controlling the balance of cellular differentiation and proliferation.

Pathways

Gas1 significantly influences the Hedgehog signaling pathway and the G1 phase of the cell cycle. In the Hedgehog pathway Gas1 interacts with other key proteins like Patched and Smoothened regulating the pathway's activity which is essential for embryonic development and tissue patterning. Its interaction with these proteins highlights its regulatory function in signal transduction influencing how cells respond to growth cues.

Gas1's dysregulation links to cancer and congenital malformations. Such disorders often occur due to alterations in the Hedgehog signaling pathway implicating Gas1 as a contributor in aberrant cell growth conditions. In cancer specifically Gas1 may influence tumor suppression mechanisms impacting cells' growth arrest. Mutations in components of the Hedgehog pathway such as Smoothened can alter Gas1's function giving rise to developmental disorders and cancer progression.
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Product protocols

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Target data

Specific growth arrest protein involved in growth suppression. Blocks entry to S phase. Prevents cycling of normal and transformed cells. Binds 20(S)-hydroxycholesterol (20(S)-OHC) (By similarity).
See full target information Growth arrest-specific protein 1
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websiteProtocolBooklet
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