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AB267602

Human GASP-1 ELISA Kit

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Human GASP-1 ELISA Kit is a Sandwich ELISA for the measurement of Human GASP-1 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

GASP, KIAA0443, GPRASP1, G-protein coupled receptor-associated sorting protein 1, GASP-1

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Sandwich ELISA - Human GASP-1 ELISA Kit (AB267602)
  • sELISA

Supplier Data

Sandwich ELISA - Human GASP-1 ELISA Kit (AB267602)

Human GASP-1 Standard curve.

These standard curves are for demonstration only. A standard curve must be run with each assay

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture media, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 20 pg/mL

Range

20.48 - 5000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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  • 5
  • 20
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human GASP-1 ELISA Kit is designed for the quantitative determination of GASP-1 in cell culture supernatants, plasma and serum samples.

This assay employs an antibody specific for human GASP-1 coated on a 96-well plate. Standards and samples are pipetted into the wells and GASP-1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human GASP-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of GASP-1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human ACE-2, aFGF, Albumin, AMICA, Angiopoietin-4, BAFF, CA19-9, CD163, Clusterin, CRTAM, CXCL14, Cystatin C, Decorin, Dkk-3, DLL1, Fetuin A, FOLR1, Furin, GASP-2, GCSF R, HAI-2, IL-17 RB, IL-27, LAG-3, LDL R, Pepsinogen 1, RANK, RBP4, SOST, Syndecan-1, TACI, TFPI, Thrombospondin 1, TRAIL R2, TRANCE, Troponin I, uPA, VECadherin, WISP-1.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
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Recovery

[ { "sample": "Serum", "range": "92 - 127 %", "average": "= 108" }, { "sample": "Plasma", "range": "89 - 123 %", "average": "= 101" }, { "sample": "Cell culture media", "range": "80 - 108 %", "average": "= 94" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab267602-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB267602-CMP02", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB267602-CMP06", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB267602-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human GASP-1 coated Microplate (12 x 8 wells)", "number":"AB267602-CMP08", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB267602-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human GASP-1 Detection Antibody", "number":"AB267602-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human GASP-1 Standard (Lyophilized)", "number":"AB267602-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB267602-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GPRASP1 also known as GASP1 stands for G-protein coupled receptor associated sorting protein 1. This protein regulates the post-endocytic sorting of G-protein coupled receptors (GPCRs). GPRASP1 interacts with various GPCRs affecting their trafficking and downregulation. Its molecular mass is approximately 125 kDa. Researchers have observed its expression in several tissues with higher levels in the brain indicating a significant role in neuronal processes.
Biological function summary

GPRASP1 plays a role in the modulation of receptor signaling by aiding in the internalization and degradation of target receptors. It functions as a part of a larger protein complex involved in receptor sorting. In the central nervous system GPRASP1 affects synaptic plasticity impacting neurological communication and receptor desensitization. This interaction highlights its regulatory influence on receptor fate after ligand binding.

Pathways

Signaling processes involve GPRASP1 particularly in the GPCR signaling pathways. GPRASP1 collaborates with proteins like beta-arrestins to mediate receptor internalization and trafficking. This protein also connects to the ubiquitination pathway by interacting with E3 ubiquitin ligases which tag receptors for degradation. These pathways demonstrate GPRASP1's pivotal position in cellular communication and receptor lifecycle management.

Researchers associate GPRASP1 with neurological conditions. Alterations in GPRASP1 function can contribute to disorders like schizophrenia and depression. GPRASP1 interaction with dopamine receptors important in mood regulation and psychotic disorders highlights this connection. These interactions have implications for pharmacological interventions aiming to modulate GPCR sensitivity and targeting in disease contexts.
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Product protocols

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Target data

Modulates lysosomal sorting and functional down-regulation of a variety of G-protein coupled receptors. Targets receptors for degradation in lysosomes via its interaction with BECN2.
See full target information GPRASP1
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websiteProtocolBooklet
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Product promise

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For full details, please see our Terms & Conditions

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