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AB314730

Human GATA-4 ELISA Kit

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Human GATA-4 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human GATA-4 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

Transcription factor GATA-4, GATA-binding factor 4, GATA4

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.85 ng/mL

Range

0.85 - 200 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human GATA-4 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human GATA-4 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human GATA-4 coated on a 96-well plate.
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Recovery

[ { "sample": "Serum", "range": "76 - 97 %", "average": "= 86.91" }, { "sample": "Plasma", "range": "68 - 86 %", "average": "= 76.24" }, { "sample": "Cell culture media", "range": "90 - 111 %", "average": "= 101.7" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GATA4 also known as GATA-binding protein 4 is a transcription factor that plays a significant role in gene expression regulation. It weighs approximately 44 kDa. This protein expresses mainly in cells of the heart gonads and liver. GATA4 functions through binding to the GATA sequence on DNA regulating various genes. The protein is well known for its involvement in cardiac development and function.
Biological function summary

GATA4 interacts with other transcription factors and co-factors to regulate developmental processes. It often forms complexes with proteins such as NKX2-5 and TBX5 influencing the expression of cardiac-specific genes. These interactions lead to tissue-specific gene expression control important in the differentiation and functioning of heart cells. GATA4 is also involved in the regulation of genes responsible for cell growth and apoptosis contributing to tissue homeostasis.

Pathways

GATA4 is a notable component of cardiac development and cellular differentiation pathways. It plays an integral role in the Wnt signaling pathway influencing heart development through gene transcription modification. GATA4 also collaborates with proteins such as MEF2 and BMP2 coordinating cardiac muscle differentiation. These pathways highlight its pivotal role in proper cardiovascular formation and function.

GATA4 mutations and dysregulation have links to congenital heart defects such as atrial septal defect (ASD). This condition associates with altered gene expression during heart development leading to structural abnormalities. GATA4 also relates to conditions like hypertrophic cardiomyopathy where its interaction with proteins like MYH7 suggests a complex network influencing cardiac hypertrophy. Understanding these connections is essential for developing targeted therapeutic strategies.
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Product protocols

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Target data

Transcriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development and function (PubMed : 24000169, PubMed : 27984724, PubMed : 35182466). In cooperation with TBX5, it binds to cardiac super-enhancers and promotes cardiomyocyte gene expression, while it down-regulates endocardial and endothelial gene expression (PubMed : 27984724). Involved in bone morphogenetic protein (BMP)-mediated induction of cardiac-specific gene expression. Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions (By similarity). Acts as a transcriptional activator of ANF in cooperation with NKX2-5 (By similarity). Promotes cardiac myocyte enlargement (PubMed : 20081228). Required during testicular development (PubMed : 21220346). May play a role in sphingolipid signaling by regulating the expression of sphingosine-1-phosphate degrading enzyme, sphingosine-1-phosphate lyase (PubMed : 15734735).
See full target information GATA4
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