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AB309395

Human GATA1 ELISA Kit

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Human GATA1 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human GATA1 in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

ERYF1, GF1, GATA1, Erythroid transcription factor, Eryf1, GATA-binding factor 1, NF-E1 DNA-binding protein, GATA-1, GF-1

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Sandwich ELISA - Human GATA1 ELISA Kit (AB309395)
  • sELISA

Supplier Data

Sandwich ELISA - Human GATA1 ELISA Kit (AB309395)

Example of standard curve using ab309395. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 16 pg/mL

Range

16.38 - 4000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human GATA1 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human GATA1 in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human GATA1 coated on a 96-well plate.
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Recovery

[ { "sample": "Plasma", "range": "67 - 79 %", "average": "= 71.13" }, { "sample": "Serum", "range": "70 - 133 %", "average": "= 94.15" }, { "sample": "Cell culture media", "range": "86 - 106 %", "average": "= 96.31" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab309395-1x96Tests", "assetComponentDetails": [ { "size":"1 x 200 µL", "name":"250X HRP-Streptavidin", "number":"AB309395-CMP12", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent D (Item K)", "number":"AB309395-CMP06", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB309395-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB309395-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human GATA-1/Eryf1 Antibody-coated ELISA Plate", "number":"AB309395-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B (Item E)", "number":"AB309395-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human GATA-1/Eryf1 Detection Antibody", "number":"AB309395-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human GATA-1/Eryf1 Protein Standard", "number":"AB309395-CMP11", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB309395-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GATA1 also known as GATA-binding factor 1 is a transcription factor weighing approximately 42 kDa. This protein binds to the DNA sequence "GATA" within the regulatory regions of target genes to control their expression. GATA1 is mostly expressed in hematopoietic cells especially erythroid cells megakaryocytes eosinophils and mast cells. Its specific expression pattern allows it to regulate genes essential for the development and function of these cell types.
Biological function summary

GATA1 regulates gene expression and is essential for erythroid and megakaryocyte differentiation. GATA1 belongs to a family of GATA transcription factors and can form regulatory complexes with other proteins such as FOG1 (Friend of GATA1) and TAL1. These complexes facilitate the binding of GATA1 to DNA enhancing its transcriptional activity on target gene sets necessary for blood cell maturation and function.

Pathways

GATA1 participates in the erythropoiesis and megakaryopoiesis pathways. It directly regulates several genes and coordinates signals with factors like GATA2 and RUNX1 which are other key proteins involved in these pathways. Through these interactions GATA1 ensures the correct development of red blood cells and platelets maintaining hematopoietic homeostasis in the organism.

Abnormal GATA1 function is linked to blood-related disorders. Mutations or dysregulations of GATA1 can lead to diseases such as X-linked thrombocytopenia with dyserythropoietic anemia which involves low platelet count and ineffective red blood cell production. Moreover GATA1 mutations are associated with certain leukemias particularly acute megakaryoblastic leukemia. GATA1's dysfunction in these diseases often occurs in conjunction with abnormalities in related proteins like GATA2 influencing disease progression and phenotype.
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Product protocols

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Target data

Transcriptional activator or repressor which serves as a general switch factor for erythroid development (PubMed : 35030251). It binds to DNA sites with the consensus sequence 5'-[AT]GATA[AG]-3' within regulatory regions of globin genes and of other genes expressed in erythroid cells. Activates the transcription of genes involved in erythroid differentiation of K562 erythroleukemia cells, including HBB, HBG1/2, ALAS2 and HMBS (PubMed : 24245781).
See full target information GATA1
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websiteProtocolBooklet
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Complementary Products

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AB181544

Anti-GATA1 antibody [EPR17362] - ChIP Grade

KO Validated|RabMAb|Recombinant

Western blot - Anti-GATA1 antibody [EPR17362] - ChIP Grade (AB181544)

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Proteins & Peptides

AB204189

Recombinant Human GATA1 protein

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