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AB325361

Human GBA ELISA Kit, Chemiluminescent

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Human GBA ELISA Kit, Chemiluminescent is a 55-min single-wash Simplestep IgniteTM ELISA for the rapid quantification of Human GBA with higher sensitivity and broader measuring range.

- Single 30 min incubation and single -wash step for results in less than an hour
- Chemiluminescent detection allows a broad dynamic range :9.14 - 20000 pg/mL with a sensitivity of 4.5 pg/mL
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Patent Pending

View Alternative Names

GBA, GC, GLUC, GBA1, Lysosomal acid glucosylceramidase, Lysosomal acid GCase, Acid beta-glucosidase, Alglucerase, Beta-glucocerebrosidase, Beta-glucosylceramidase 1, Cholesterol glucosyltransferase, Cholesteryl-beta-glucosidase, D-glucosyl-N-acylsphingosine glucohydrolase, Glucosylceramidase beta 1, Imiglucerase, Lysosomal cholesterol glycosyltransferase, Lysosomal galactosylceramidase, Lysosomal glycosylceramidase, Beta-GC, SGTase

8 Images
Sandwich ELISA - Human GBA ELISA Kit, Chemiluminescent (AB325361)
  • sELISA

Lab

Sandwich ELISA - Human GBA ELISA Kit, Chemiluminescent (AB325361)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Human GBA ELISA Kit, Chemiluminescent (AB325361)
  • sELISA

Lab

Sandwich ELISA - Human GBA ELISA Kit, Chemiluminescent (AB325361)

Interpolated concentration of native GBA was measured in duplicate at different sample concentrations. Undiluted samples are as follows : LNCaP extract 5 µg/mL and kidney extract 50 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Human GBA ELISA Kit, Chemiluminescent (AB325361)
  • sELISA

Lab

Sandwich ELISA - Human GBA ELISA Kit, Chemiluminescent (AB325361)

Standard curve comparison between the Chemiluminescent Human GBA SimpleStep ELISA and colorimetric Human GBA SimpleStep ELISA (ab315298) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human GBA ELISA Kit, Chemiluminescent (AB325361)
  • sELISA

Lab

Sandwich ELISA - Human GBA ELISA Kit, Chemiluminescent (AB325361)

Serum of ten individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean GBA concentration was determined to be 15.3 ng/mL with a range of 8.7 – 24.3 ng/mL.

Sandwich ELISA - Human GBA ELISA Kit, Chemiluminescent (AB325361)
  • sELISA

Lab

Sandwich ELISA - Human GBA ELISA Kit, Chemiluminescent (AB325361)

Standard curve comparison between the Chemiluminescent Human GBA SimpleStep ELISA and colorimetric Human GBA SimpleStep ELISA (ab315298) in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human GBA ELISA Kit, Chemiluminescent (AB325361)
  • sELISA

Lab

Sandwich ELISA - Human GBA ELISA Kit, Chemiluminescent (AB325361)

Interpolated concentration of native GBA was measured in duplicate at different sample concentrations. Undiluted samples are 1 : 16 human serum, plasma (citrate), plasma (EDTA), and plasma (heparin). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human GBA ELISA Kit, Chemiluminescent (AB325361)
  • sELISA

Lab

Sandwich ELISA - Human GBA ELISA Kit, Chemiluminescent (AB325361)

The concentration of GBA was measured in human wild type and knockout HeLa cell extracts based on a 12.5 µg/mL extract load. The mean concentration was determined to be 3,620 pg/mL in wild type extract and undetectable (ND) in knockout extract. The control and GBA knockout cell lines are available as ab265038.

Sandwich ELISA - Human GBA ELISA Kit, Chemiluminescent (AB325361)
  • sELISA

Lab

Sandwich ELISA - Human GBA ELISA Kit, Chemiluminescent (AB325361)

Dynamic range comparison between the Chemiluminescent Human GBA SimpleStep ELISA, colorimetric Human GBA SimpleStep ELISA (ab315298), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Key facts

Detection method

Luminescent

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell Extract, Tissue Extracts

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 4.5 pg/mL

Range

9.14 - 20000 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human GBA ELISA Kit, Chemiluminescent (ab325361) is a rapid single-wash sandwich 55-min ELISA to measure Human GBA in Serum, Citrate Plasma, EDTA Plasma, Heparin Plasma, Cell Extract, Tissue Extract with a sensitivity of 4.5 pg/ml.

How the assay works

Human GBA ELISA Kit, Chemiluminescent (ab325361) SimpleStep ELISA®  employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time.  By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colormetric ELISAs.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.

Please refer to our protocol booklet for more details.

Human GBA (ab325361) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature

2. Wash

3. Add prepared ChemiHRP development solution - incubate for 2 min

4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Rabbit monoclonal [EPR26755-29] to GBA - BSA and Azide free (Capture) ab309228

- Rabbit monoclonal [EPR26755-42] to GBA - BSA and Azide free (Detector) ab309229

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.4" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.9" } ]
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Recovery

[ { "sample": "Serum", "range": "89 - 121 %", "average": "= 103" }, { "sample": "Citrate plasma", "range": "85 - 97 %", "average": "= 90" }, { "sample": "EDTA Plasma", "range": "95 - 126 %", "average": "= 107" }, { "sample": "Heparin Plasma", "range": "94 - 102 %", "average": "= 98" }, { "sample": "Cell Lysate", "range": "94 - 108 %", "average": "= 100" }, { "sample": "Tissue Extracts", "range": "88 - 119 %", "average": "= 107" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab325361-1x96Tests", "assetComponentDetails": [ { "size":"1 x 3 mL", "name":"ChemiHRP Reagent A", "number":"AB325361-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated Black 96-Well Microplate", "number":"AB325361-CMP10", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent B", "number":"AB325361-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human GBA Detector Antibody", "number":"AB325361-CMP02", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB325361-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human GBA Capture Antibody", "number":"AB325361-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB325361-CMP11", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB325361-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human GBA Lyophilized Recombinant Protein", "number":"AB325361-CMP03", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB325361-CMP05", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB325361-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Glucosylceramidase that catalyzes, within the lysosomal compartment, the hydrolysis of glucosylceramides/GlcCers (such as beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine) into free ceramides (such as N-acylsphing-4-enine) and glucose (PubMed : 15916907, PubMed : 24211208, PubMed : 32144204, PubMed : 9201993). Plays a central role in the degradation of complex lipids and the turnover of cellular membranes (PubMed : 27378698). Through the production of ceramides, participates in the PKC-activated salvage pathway of ceramide formation (PubMed : 19279011). Catalyzes the glucosylation of cholesterol, through a transglucosylation reaction where glucose is transferred from GlcCer to cholesterol (PubMed : 24211208, PubMed : 26724485, PubMed : 32144204). GlcCer containing mono-unsaturated fatty acids (such as beta-D-glucosyl-N-(9Z-octadecenoyl)-sphing-4-enine) are preferred as glucose donors for cholesterol glucosylation when compared with GlcCer containing same chain length of saturated fatty acids (such as beta-D-glucosyl-N-octadecanoyl-sphing-4-enine) (PubMed : 24211208). Under specific conditions, may alternatively catalyze the reverse reaction, transferring glucose from cholesteryl 3-beta-D-glucoside to ceramide (Probable) (PubMed : 26724485). Can also hydrolyze cholesteryl 3-beta-D-glucoside producing glucose and cholesterol (PubMed : 24211208, PubMed : 26724485). Catalyzes the hydrolysis of galactosylceramides/GalCers (such as beta-D-galactosyl-(1<->1')-N-acylsphing-4-enine), as well as the transfer of galactose between GalCers and cholesterol in vitro, but with lower activity than with GlcCers (PubMed : 32144204). Contrary to GlcCer and GalCer, xylosylceramide/XylCer (such as beta-D-xyosyl-(1<->1')-N-acylsphing-4-enine) is not a good substrate for hydrolysis, however it is a good xylose donor for transxylosylation activity to form cholesteryl 3-beta-D-xyloside (PubMed : 33361282).
See full target information GBA1
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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