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AB108853

Human GC Globulin ELISA Kit (Vitamin D Binding Protein)

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(6 Publications)

Human GC Globulin ELISA Kit (Vitamin D Binding Protein) is a Competitive ELISA for the measurement of Human GC Globulin (Vitamin D Binding Protein) in Human in Biofluids samples.

View Alternative Names

Vitamin D-binding protein, DBP, VDB, Gc protein-derived macrophage activating factor, Gc-globulin, Group-specific component, Vitamin D-binding protein-macrophage activating factor, Gc-MAF, GcMAF, Gc, DBP-maf, GC

1 Images
Competitive ELISA - Human GC Globulin ELISA Kit (Vitamin D Binding Protein) (AB108853)
  • cELISA

Supplier Data

Competitive ELISA - Human GC Globulin ELISA Kit (Vitamin D Binding Protein) (AB108853)

Representative Standard Curve using ab108853.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum

Reacts with

Human

Assay type

Competitive

Sensitivity

= 67 ng/mL

Range

0.098 - 100 µg/mL

Assay time

3h

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Human GC Globulin (Vitamin D Binding Protein) in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitive measurement of GC Globulin in plasma and serum.

A GC Globulin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated GC Globulin is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of GC Globulin captured in plate.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.6" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Product protocols

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Target data

Involved in vitamin D transport and storage, scavenging of extracellular G-actin, enhancement of the chemotactic activity of C5 alpha for neutrophils in inflammation and macrophage activation.
See full target information GC
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Translational vision science & technology 11:16 PubMed36580321

2022

Vitamin D Supplementation Impacts Systemic Biomarkers of Collagen Degradation and Copper Metabolism in Patients With Keratoconus.

Applications

Unspecified application

Species

Unspecified reactive species

Romina Mayra Lasagni Vitar,Philippe Fonteyne,Karl Anders Knutsson,Federico Bertuzzi,Laura Galli,Paolo Rama,Giulio Ferrari

Nutrients 13: PubMed34836178

2021

Prevalence of Micronutrient Deficiencies and Relationship with Clinical and Patient-Related Outcomes in Pulmonary Hypertension Types I and IV.

Applications

Unspecified application

Species

Unspecified reactive species

Paulien Vinke,Thomas Koudstaal,Femke Muskens,Annemien van den Bosch,Michiel Balvers,Mieke Poland,Renger F Witkamp,Klaske van Norren,Karin A Boomars

Nutrients 12: PubMed32824958

2020

Relationships between Total, Free and Bioavailable Vitamin D and Vitamin D Binding Protein in Early Pregnancy with Neonatal Outcomes: A Retrospective Cohort Study.

Applications

Unspecified application

Species

Unspecified reactive species

Melinda Fernando,Thisara G Coster,Stacey J Ellery,Deborah de Guingand,Siew Lim,Cheryce L Harrison,Helena J Teede,Negar Naderpoor,Aya Mousa

Journal of clinical medicine 9: PubMed32664376

2020

Early Pregnancy Vitamin D Binding Protein Is Independently Associated with the Development of Gestational Diabetes: A Retrospective Cohort Study.

Applications

Unspecified application

Species

Unspecified reactive species

Melinda Fernando,Stacey J Ellery,Deborah de Guingand,Clara Marquina,Siew Lim,Cheryce L Harrison,Helena J Teede,Negar Naderpoor,Aya Mousa

Nutrients 12: PubMed32357490

2020

The Relationship between Vitamin D Metabolites and Androgens in Women with Polycystic Ovary Syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Jakub Mesinovic,Helena J Teede,Soulmaz Shorakae,Gavin W Lambert,Elisabeth A Lambert,Negar Naderpoor,Barbora de Courten

The Journal of steroid biochemistry and molecular biology 177:209-215 PubMed28734987

2017

Bioavailable and free 25-hydroxyvitamin D and vitamin D binding protein in polycystic ovary syndrome: Relationships with obesity and insulin resistance.

Applications

cELISA

Species

Unspecified reactive species

Negar Naderpoor,Soulmaz Shorakae,Sally K Abell,Aya Mousa,Anju E Joham,Lisa J Moran,Nigel K Stepto,Poli Mara Spritzer,Helena J Teede,Barbora de Courten
View all publications
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Product promise

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