Human GCDFP 15 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human GCDFP 15 in Human in Saliva, Urine, Plasma, Cell culture supernatant, Milk, Serum samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Saliva, Urine, Plasma, Cell culture supernatant, Milk, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.625 - 20 ng/mL
- Assay time
- 4h
- Sensitivity
- = 0.23 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Alternative names
GCDFP15, GPIP4, PIP, Prolactin-inducible protein, Gross cystic disease fluid protein 15, Prolactin-induced protein, Secretory actin-binding protein, gp17, GCDFP-15, SABP
What's included?
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Human GCDFP 15 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human GCDFP 15 in Human in Saliva, Urine, Plasma, Cell culture supernatant, Milk, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Saliva, Urine, Plasma, Cell culture supernatant, Milk, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.625 - 20 ng/mL
- Assay time
- 4h
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 0.23 ng/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 60 | mean - | SD - | C.V. 4.5 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 60 | mean - | SD - | C.V. 8.2 |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- Multi
- Storage information
- Please refer to protocols
Notes
The Human PIP ELISA (ab284017) Kit is designed for detection of PIP in human plasma, serum, milk, urine, saliva, and cell culture samples.
This assay employs a quantitative sandwich enzyme immunoassay technique that measures human PIP in approximately 4 hours. A polyclonal antibody specific for human PIP has been pre-coated onto a 96- well microplate with removable strips. PIP in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human PIP, which is recognized by a streptavidinperoxidase (SP) conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GCDFP-15 also known as gross cystic disease fluid protein 15 is a glycoprotein with a molecular weight of approximately 15 kDa. It is commonly expressed in apocrine glands particularly in breast tissue. Immunohistochemical studies show that GCDFP-15 is a marker for breast apocrine metaplasia and is frequently used in identifying apocrine carcinoma. The presence of GCDFP-15 in other sites like salivary glands suggests its broader role within glandular tissues.
- Biological function summary
GCDFP-15 functions as a secretory protein involved in the regulation of fluid composition in ducts. It is not known to be part of a larger complex but acts independently. This protein plays a role in stabilizing the microenvironment of ductal secretions which assists in the preservation of ductal lining and the maintenance of tissue integrity. The modulation of fluid content by GCDFP-15 is essential in maintaining the function of glandular tissues.
- Pathways
GCDFP-15 is integral to lipid metabolism pathways and may indirectly influence signal transduction. It shows interaction with other proteins like prolactin and fatty acid-binding protein suggesting a broader role in lipid-related biological processes. The involvement of GCDFP-15 in these pathways indicates its contribution to the regulation of metabolic homeostasis within glandular tissues.
- Associated diseases and disorders
GCDFP-15 has notable significance in breast cancer particularly in differentiating apocrine-type carcinoma from other subtypes. The expression of GCDFP-15 is considered a biomarker in breast pathology for diagnostic purposes. In addition its role in salivary gland disorders is under investigation given its expression in the ducts of salivary glands. The protein's relationship with markers like mammaglobin further highlights its clinical relevance in distinguishing between glandular-related pathologies.
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1 product image
Sandwich ELISA - Human GCDFP 15 ELISA Kit (ab284017)
Example of Human GCDFP 15 standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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