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AB284017

Human GCDFP 15 ELISA Kit

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Human GCDFP 15 ELISA Kit is a Sandwich ELISA for the measurement of Human GCDFP 15 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

GCDFP15, GPIP4, PIP, Prolactin-inducible protein, Gross cystic disease fluid protein 15, Prolactin-induced protein, Secretory actin-binding protein, gp17, GCDFP-15, SABP

1 Images
Sandwich ELISA - Human GCDFP 15 ELISA Kit (AB284017)
  • sELISA

Supplier Data

Sandwich ELISA - Human GCDFP 15 ELISA Kit (AB284017)

Example of Human GCDFP 15 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Saliva, Urine, Plasma, Cell culture supernatant, Milk, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.23 ng/mL

Range

0.625 - 20 ng/mL

Assay time

4h

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Primary Antibodies

AB32028

Anti-mTOR antibody [Y391]

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Primary Antibodies

AB31488

Anti-CARD15/NOD2 antibody [2D9]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARD15/NOD2 antibody [2D9] (AB31488)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

The Human PIP ELISA (ab284017) Kit is designed for detection of PIP in human plasma, serum, milk, urine, saliva, and cell culture samples.

This assay employs a quantitative sandwich enzyme immunoassay technique that measures human PIP in approximately 4 hours. A polyclonal antibody specific for human PIP has been pre-coated onto a 96- well microplate with removable strips. PIP in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human PIP, which is recognized by a streptavidinperoxidase (SP) conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 60, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.2" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 60, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.5" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab284017-1x96Tests", "assetComponentDetails": [ { "size":"2 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB284017-CMP06", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB284017-CMP10", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB284017-CMP09", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB284017-CMP03", "productcode":"" }, { "size":"1 x 120 µL", "name":"50X Biotinylated Human GCDFP 15 Antibody", "number":"AB284017-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human GCDFP 15 Microplate", "number":"AB284017-CMP05", "productcode":"" }, { "size":"1 x 30 mL", "name":"10X Diluent N Concentrate", "number":"AB284017-CMP01", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB284017-CMP08", "productcode":"" }, { "size":"1 x 2 mL", "name":"1X Standard Diluent", "number":"AB284017-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"GCDFP 15 Standard", "number":"AB284017-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GCDFP-15 also known as gross cystic disease fluid protein 15 is a glycoprotein with a molecular weight of approximately 15 kDa. It is commonly expressed in apocrine glands particularly in breast tissue. Immunohistochemical studies show that GCDFP-15 is a marker for breast apocrine metaplasia and is frequently used in identifying apocrine carcinoma. The presence of GCDFP-15 in other sites like salivary glands suggests its broader role within glandular tissues.
Biological function summary

GCDFP-15 functions as a secretory protein involved in the regulation of fluid composition in ducts. It is not known to be part of a larger complex but acts independently. This protein plays a role in stabilizing the microenvironment of ductal secretions which assists in the preservation of ductal lining and the maintenance of tissue integrity. The modulation of fluid content by GCDFP-15 is essential in maintaining the function of glandular tissues.

Pathways

GCDFP-15 is integral to lipid metabolism pathways and may indirectly influence signal transduction. It shows interaction with other proteins like prolactin and fatty acid-binding protein suggesting a broader role in lipid-related biological processes. The involvement of GCDFP-15 in these pathways indicates its contribution to the regulation of metabolic homeostasis within glandular tissues.

GCDFP-15 has notable significance in breast cancer particularly in differentiating apocrine-type carcinoma from other subtypes. The expression of GCDFP-15 is considered a biomarker in breast pathology for diagnostic purposes. In addition its role in salivary gland disorders is under investigation given its expression in the ducts of salivary glands. The protein's relationship with markers like mammaglobin further highlights its clinical relevance in distinguishing between glandular-related pathologies.
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Product protocols

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Target data

See full target information PIP
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websiteProtocolBooklet
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Product promise

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