AB233632

Human GCLC ELISA Kit

Recombinant
SimpleStep
What is this?

Be the first to review this product! Submit a review

(3 Publications)

Human GCLC ELISA Kit is a single-wash 90-min Simplestep used to quantify Human GCLC with a sensitivity of 81 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

GLCL, GLCLC, GCLC, Glutamate--cysteine ligase catalytic subunit, GCS heavy chain, Gamma-ECS, Gamma-glutamylcysteine synthetase

3 Images

Sandwich ELISA - Human GCLC ELISA Kit (AB233632)

sELISA

Unknown

Sandwich ELISA - Human GCLC ELISA Kit (AB233632)

Interpolated concentrations of native GCLC in MCF-7 cell extract samples based on a 1,000 µg/mL extract load.

The concentrations of GCLC were measured in duplicate and interpolated from the GCLC standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean GCLC concentration was determined to be 3,074 pg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human GCLC ELISA Kit (AB233632)

Example of human GCLC standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human GCLC ELISA Kit (AB233632)

Representative image of Human GCLC ELISA Kit (ab233632)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Human GCLC ELISA Kit Capture Antibody

- 10X Human GCLC Detector Antibody

- 10X Human GCLC ELISA Kit Lyophilised Purified Protein (2 vials)

- Human GCLC ELISA Kit 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody Diluent 4BI

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 81 pg/mL

Range

781.3 - 50000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

Product details

Human GCLC ELISA Kit (ab233632) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of GCLC protein in cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human GCLC with 81 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style

left-column

Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7" } ]

style

left-column

Recovery

[ { "sample": "Cell culture extracts", "range": null, "average": "= 95" } ]

style

left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab233632-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB233632-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB233632-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB233632-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB233632-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB233632-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB233632-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human GCLC Capture Antibody", "number":"AB233632-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human GCLC Lyophilized Recombinant Protein", "number":"AB233632-CMP11", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB233632-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB233632-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human GCLC Detector Antibody", "number":"AB233632-CMP10", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB233632-CMP06", "productcode":"" } ] } } }

style

left-column

Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

style

left-column

style

left-column

Product protocols

style

left-column

Target data

Catalyzes the ATP-dependent ligation of L-glutamate and L-cysteine and participates in the first and rate-limiting step in glutathione biosynthesis.

See full target information GCLC

style

left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Free radical biology & medicine 188:105-116 PubMed35718303

2022

The role of cycloastragenol at the intersection of NRF2/ARE, telomerase, and proteasome activity.

Applications

Unspecified application

Species

Unspecified reactive species

Sinem Yilmaz,Erdal Bedir,Petek Ballar Kirmizibayrak

Marine drugs 20: PubMed35049870

2021

Fucoxanthin Pretreatment Ameliorates Visible Light-Induced Phagocytosis Disruption of RPE Cells under a Lipid-Rich Environment via the Nrf2 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yunjun Liu,Zixin Guo,Shengnan Wang,Yixiang Liu,Ying Wei

Redox biology 41:101947 PubMed33774476

2021

NOX4 promotes ferroptosis of astrocytes by oxidative stress-induced lipid peroxidation via the impairment of mitochondrial metabolism in Alzheimer's diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Min Woo Park,Hyeon Woo Cha,Junhyung Kim,Jung Han Kim,Haesung Yang,Sunmi Yoon,Napissara Boonpraman,Sun Shin Yi,Ik Dong Yoo,Jong-Seok Moon

View all publications

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human GCLC ELISA Kit

Sandwich ELISA - Human GCLC ELISA Kit (AB233632)

Sandwich ELISA - Human GCLC ELISA Kit (AB233632)

Sandwich ELISA - Human GCLC ELISA Kit (AB233632)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (3)

Complementary Products

Assay Kits

Secondary Antibodies

Assay Kits

Primary Antibodies

Primary Antibodies

Product promise