Human GCNT1 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human GCNT1 in Human in Serum, Plasma, Cell culture supernatant samples.
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Plasma, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.35 - 80 ng/mL
- Sensitivity
- = 0.35 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Glycosyltransferase that catalyzes the transfer of an N-acetylglucosamine (GlcNAc) moiety in beta1-6 linkage from UDP-GlcNAc onto mucin-type core 1 O-glycan to form the branched mucin-type core 2 O-glycan (PubMed:1329093, PubMed:23027862). The catalysis is metal ion-independent and occurs with inversion of the anomeric configuration of sugar donor (By similarity). Selectively involved in synthesis of mucin-type core 2 O-glycans that serve as scaffolds for the display of selectin ligand sialyl Lewis X epitope by myeloid cells, with an impact on homeostasis and recruitment to inflammatory sites (By similarity). Can also act on glycolipid substrates. Transfers GlcNAc moiety to GalGb4Cer globosides in a reaction step to the synthesis of stage-specific embryonic antigen 1 (SSEA-1) determinant (By similarity). Can use Galbeta1-3GalNAcalpha1- and Galbeta1-3GalNAcbeta1- oligosaccharide derivatives as acceptor substrates (By similarity).
Alternative names
NACGT2, GCNT1, Core 2-branching enzyme, Core2-GlcNAc-transferase, C2GlcNAcT, C2GNT, Core 2 GNT, C2GnT-L
What's included?
Recommended products
Human GCNT1 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human GCNT1 in Human in Serum, Plasma, Cell culture supernatant samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Plasma, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.35 - 80 ng/mL
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 0.35 ng/mL
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 105.9 | Range 100 - 114 % |
Sample type Plasma | Average % = 98.63 | Range 84 - 111 % |
Sample type Cell culture media | Average % = 101.6 | Range 79 - 122 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Human GCNT1 ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human GCNT1 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human GCNT1 coated on a 96-well plate.
This ELISA kit shows no cross-reactivity with the following cytokines tested: human Activin C, AHCY, APE, Bax, BNIP3, Caspase-3, Cathepsin F, CD177, CD96, CLEC4D, C-Peptide, DCBLD2, Dystroglycan, FAM3A, GLA, HAPLN4, hCG alpha, HMGB3, IkB-E, IRF4, KIR3DL1, Klotho beta, Lck, LILRA5, METTL11A, p55PIK, PCDH17, PLA2G4A, RalB, S100A2, S100B, SH2B1, Siglec-3, SOD3, ST8SIA2, Testican 1, TRP14, VAP-B, ZAP70.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
Sandwich ELISA - Human GCNT1 ELISA Kit (ab313963)
Example of standard curve using a313963. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com