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AB313963

Human GCNT1 ELISA Kit

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Human GCNT1 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human GCNT1 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

NACGT2, GCNT1, Core 2-branching enzyme, Core2-GlcNAc-transferase, C2GlcNAcT, C2GNT, Core 2 GNT, C2GnT-L

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Sandwich ELISA - Human GCNT1 ELISA Kit (AB313963)
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Supplier Data

Sandwich ELISA - Human GCNT1 ELISA Kit (AB313963)

Example of standard curve using a313963. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.35 ng/mL

Range

0.35 - 80 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human GCNT1 ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human GCNT1 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human GCNT1 coated on a 96-well plate.

This ELISA kit shows no cross-reactivity with the following cytokines tested: human Activin C, AHCY, APE, Bax, BNIP3, Caspase-3, Cathepsin F, CD177, CD96, CLEC4D, C-Peptide, DCBLD2, Dystroglycan, FAM3A, GLA, HAPLN4, hCG alpha, HMGB3, IkB-E, IRF4, KIR3DL1, Klotho beta, Lck, LILRA5, METTL11A, p55PIK, PCDH17, PLA2G4A, RalB, S100A2, S100B, SH2B1, Siglec-3, SOD3, ST8SIA2, Testican 1, TRP14, VAP-B, ZAP70.

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Recovery

[ { "sample": "Serum", "range": "100 - 114 %", "average": "= 105.9" }, { "sample": "Plasma", "range": "84 - 111 %", "average": "= 98.63" }, { "sample": "Cell culture media", "range": "79 - 122 %", "average": "= 101.6" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab313963-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB313963-CMP04", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB313963-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB313963-CMP07", "productcode":"" }, { "size":"1 x 200 µL", "name":"100X HRP-Streptavidin", "number":"AB313963-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human GCNT1 Antibody-coated ELISA Plate", "number":"AB313963-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB313963-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human GCNT1 Protein Standard", "number":"AB313963-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human GCNT1 Detection Antibody", "number":"AB313963-CMP09", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Glycosyltransferase that catalyzes the transfer of an N-acetylglucosamine (GlcNAc) moiety in beta1-6 linkage from UDP-GlcNAc onto mucin-type core 1 O-glycan to form the branched mucin-type core 2 O-glycan (PubMed : 1329093, PubMed : 23027862). The catalysis is metal ion-independent and occurs with inversion of the anomeric configuration of sugar donor (By similarity). Selectively involved in synthesis of mucin-type core 2 O-glycans that serve as scaffolds for the display of selectin ligand sialyl Lewis X epitope by myeloid cells, with an impact on homeostasis and recruitment to inflammatory sites (By similarity). Can also act on glycolipid substrates. Transfers GlcNAc moiety to GalGb4Cer globosides in a reaction step to the synthesis of stage-specific embryonic antigen 1 (SSEA-1) determinant (By similarity). Can use Galbeta1-3GalNAcalpha1- and Galbeta1-3GalNAcbeta1- oligosaccharide derivatives as acceptor substrates (By similarity).
See full target information GCNT1
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websiteProtocolBooklet
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Complementary Products

Primary Antibodies

AB102665

Anti-GCNT1 antibody

Western blot - Anti-GCNT1 antibody (AB102665)

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