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Human GCP-2 ELISA Kit (CXCL6) is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human GCP-2 (CXCL6) in Cell culture media, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.

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Images

Sandwich ELISA - Human GCP-2 ELISA Kit (CXCL6) (AB260055), expandable thumbnail
  • Sandwich ELISA - Human GCP-2 ELISA Kit (CXCL6) (AB260055), expandable thumbnail
  • Sandwich ELISA - Human GCP-2 ELISA Kit (CXCL6) (AB260055), expandable thumbnail

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

25 - 1600 pg/mL

Assay time

1h 30m

Sensitivity

= 13.1 pg/mL

Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Chemotactic for neutrophil granulocytes. Signals through binding and activation of its receptors (CXCR1 and CXCR2). In addition to its chemotactic and angiogenic properties, it has strong antibacterial activity against Gram-positive and Gram-negative bacteria (90-fold-higher when compared to CXCL5 and CXCL7).

Alternative names

What's included?

1 x 96 Tests
Components
10X Human GCP-2 (CXCL6) Capture Antibody
1 x 600 µL
10X Human GCP-2 (CXCL6) Detector Antibody
1 x 600 µL
10X Wash Buffer PT (ab206977)
1 x 20 mL
Antibody Diluent 5BI
1 x 6 mL
Human GCP-2 (CXCL6) Lyophilized Recombinant Protein
2 x 1 Vial
Plate Seals
1 x 1 Unit
Sample Diluent NS (ab193972)
1 x 50 mL
SimpleStep Pre-Coated 96-Well Microplate (ab206978)
1 x 1 Unit
Stop Solution
1 x 12 mL
TMB Development Solution
1 x 12 mL

Recommended products

Human GCP-2 ELISA Kit (CXCL6) is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human GCP-2 (CXCL6) in Cell culture media, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

25 - 1600 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Sensitivity

= 13.1 pg/mL

Precision

Intra assay

Sample

Serum

n

8

mean

-

SD

-

C.V.

3.1

Inter assay

Sample

Serum

n

3

mean

-

SD

-

C.V.

2.4

Recovery

Sample specific recovery

Sample type

Cell culture supernatant

Average %

= 94

Range

90 - 101 %

Sample type

Serum

Average %

= 86

Range

83 - 88 %

Sample type

EDTA Plasma

Average %

= 81

Range

78 - 85 %

Sample type

Cell culture media

Average %

= 99

Range

93 - 105 %

Sample type

Citrate plasma

Average %

= 95

Range

94 - 96 %

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Human GCP-2 ELISA Kit (CXCL6) (ab260055) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of GCP-2 (CXCL6) protein in cit plasma, edta plasma, serum, cell culture media, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human GCP-2 (CXCL6) with 13.1 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

GCP-2 (Granulocyte chemotactic protein 2), also known as CXCL6 (C-X-C motif chemokine 6) is a chemotactic factor for neutrophil granulocytes. GCP-2 signals through binding and activation of its receptors CXCR1 and CXCR2. In addition to its chemotactic and angiogenic properties, it has strong antibacterial activity against Gram-positive and Gram-negative bacteria.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Sandwich ELISA - Human GCP-2 ELISA Kit (CXCL6) (ab260055), expandable thumbnail

    Sandwich ELISA - Human GCP-2 ELISA Kit (CXCL6) (ab260055)

    Human GCP-2 standard curve comparison.

    Standard Curve comparison between human GCP-2 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

  • Sandwich ELISA - Human GCP-2 ELISA Kit (CXCL6) (ab260055), expandable thumbnail

    Sandwich ELISA - Human GCP-2 ELISA Kit (CXCL6) (ab260055)

    Example of human GCP-2 standard curve in Sample Diluent NS.

    The GCP-2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • Sandwich ELISA - Human GCP-2 ELISA Kit (CXCL6) (ab260055), expandable thumbnail

    Sandwich ELISA - Human GCP-2 ELISA Kit (CXCL6) (ab260055)

    Interpolated concentrations of native GCP-2 in human PBMC cell culture supernatants derived from cells seeded at 107 cells/mL and cultured in the presence or absence of 1.5% PHA for 36 hours.

    The concentrations of GCP-2 were measured in duplicates, interpolated from the GCP-2 standard curves and corrected for sample dilution. Undiluted samples are as follows: PBMC cell culture supernatant (stimulated) 50% and PBMC cell culture supernatant (unstimulated) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean GCP-2 concentration was determined to be 1,064 pg/mL in neat stimulated PBMC cell culture supernatant and undetectable in unstimulated PBMC cell culture supernatant.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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