AB260055

Human GCP-2 ELISA Kit (CXCL6)

Recombinant
SimpleStep
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Human GCP-2 ELISA Kit (CXCL6) is a single-wash 90-min Simplestep used to quantify Human GCP-2 (CXCL6) with a sensitivity of 13.1 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

GCP2, SCYB6, CXCL6, C-X-C motif chemokine 6, Chemokine alpha 3, Granulocyte chemotactic protein 2, Small-inducible cytokine B6, CKA-3, GCP-2

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ELISA - Human GCP-2 ELISA Kit (CXCL6) (AB260055)

ELISA

Supplier Data

ELISA - Human GCP-2 ELISA Kit (CXCL6) (AB260055)

sELISA

Supplier Data

Sandwich ELISA - Human GCP-2 ELISA Kit (CXCL6) (AB260055)

Interpolated concentrations of native GCP-2 in human PBMC cell culture supernatants derived from cells seeded at 10⁷ cells/mL and cultured in the presence or absence of 1.5% PHA for 36 hours.

The concentrations of GCP-2 were measured in duplicates, interpolated from the GCP-2 standard curves and corrected for sample dilution. Undiluted samples are as follows : PBMC cell culture supernatant (stimulated) 50% and PBMC cell culture supernatant (unstimulated) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean GCP-2 concentration was determined to be 1,064 pg/mL in neat stimulated PBMC cell culture supernatant and undetectable in unstimulated PBMC cell culture supernatant.

sELISA

Supplier Data

Sandwich ELISA - Human GCP-2 ELISA Kit (CXCL6) (AB260055)

Human GCP-2 standard curve comparison.

Standard Curve comparison between human GCP-2 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

sELISA

Supplier Data

Sandwich ELISA - Human GCP-2 ELISA Kit (CXCL6) (AB260055)

Example of human GCP-2 standard curve in Sample Diluent NS.

The GCP-2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 13.1 pg/mL

Range

25 - 1600 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human GCP-2 ELISA Kit (CXCL6) ab260055 is a rapid single-wash 90-min Sandwich ELISA to measure Human GCP-2 (CXCL6)in cell culture media, cell culture supernatant, citrate plasma, EDTA plasma, serum. This SimpleStep sensitivity is 13.1 pg/mL.

How the assay works

Human GCP-2 (CXCL6)SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human GCP-2 ELISA Kit (CXCL6) ab260055 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-GCP2 antibody [EPR22310-215] - BSA and Azide free (Capture) ab259523
- Anti-GRO gamma + GCP2 antibody [EPR22310-120] - BSA and Azide free (Detector) ab259523

GCP-2 (Granulocyte chemotactic protein 2), also known as CXCL6 (C-X-C motif chemokine 6) is a chemotactic factor for neutrophil granulocytes. GCP-2 signals through binding and activation of its receptors CXCR1 and CXCR2. In addition to its chemotactic and angiogenic properties, it has strong antibacterial activity against Gram-positive and Gram-negative bacteria.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.4" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.1" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "90 - 101 %", "average": "= 94" }, { "sample": "Serum", "range": "83 - 88 %", "average": "= 86" }, { "sample": "EDTA Plasma", "range": "78 - 85 %", "average": "= 81" }, { "sample": "Cell culture media", "range": "93 - 105 %", "average": "= 99" }, { "sample": "Citrate plasma", "range": "94 - 96 %", "average": "= 95" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab260055-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB260055-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB260055-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB260055-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB260055-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB260055-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human GCP-2 (CXCL6) Capture Antibody", "number":"AB260055-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human GCP-2 (CXCL6) Lyophilized Recombinant Protein", "number":"AB260055-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB260055-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human GCP-2 (CXCL6) Detector Antibody", "number":"AB260055-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB260055-CMP05", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Chemotactic for neutrophil granulocytes. Signals through binding and activation of its receptors (CXCR1 and CXCR2). In addition to its chemotactic and angiogenic properties, it has strong antibacterial activity against Gram-positive and Gram-negative bacteria (90-fold-higher when compared to CXCL5 and CXCL7).

See full target information CXCL6

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human GCP-2 ELISA Kit (CXCL6)

ELISA - Human GCP-2 ELISA Kit (CXCL6) (AB260055)

Sandwich ELISA - Human GCP-2 ELISA Kit (CXCL6) (AB260055)

Sandwich ELISA - Human GCP-2 ELISA Kit (CXCL6) (AB260055)

Sandwich ELISA - Human GCP-2 ELISA Kit (CXCL6) (AB260055)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

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