Human GDF 9 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human GDF 9 in Human in Serum, Plasma, Cell culture supernatant samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Plasma, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 2.2 - 500 ng/mL
- Sensitivity
- = 2.2 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Required for ovarian folliculogenesis. Promotes primordial follicle development. Stimulates granulosa cell proliferation. Promotes cell transition from G0/G1 to S and G2/M phases, through an increase of CCND1 and CCNE1 expression, and RB1 phosphorylation. It regulates STAR expression and cAMP-dependent progesterone release in granulosa and thecal cells. Attenuates the suppressive effects of activin A on STAR expression and progesterone production by increasing the expression of inhibin B. It suppresses FST and FSTL3 production in granulosa-lutein cells.
Alternative names
Growth/differentiation factor 9, GDF-9, GDF9
What's included?
Recommended products
Human GDF 9 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human GDF 9 in Human in Serum, Plasma, Cell culture supernatant samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Plasma, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 2.2 - 500 ng/mL
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 2.2 ng/mL
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 126.7 | Range 112 - 140 % |
Sample type Plasma | Average % = 110 | Range 77 - 147 % |
Sample type Cell culture media | Average % = 128.5 | Range 124 - 133 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Human GDF 9 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human GDF 9 in serum (human GDF 9 concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma (Collect plasma using Citrate and Heparin as an anticoagulant. EDTA is not recommended as anticoagulants for use in this assay) and cell culture supernatants. This assay employs an antibody specific for human GDF 9 coated on a 96-well plate.
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1 product image
Sandwich ELISA - Human GDF 9 ELISA Kit (ab314731)
Example of standard curve using ab314731. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.
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Product protocols
- Visit the general protocols
- Visit troubleshooting
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