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AB314731

Human GDF 9 ELISA Kit

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Human GDF 9 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human GDF 9 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

Growth/differentiation factor 9, GDF-9, GDF9

1 Images
Sandwich ELISA - Human GDF 9 ELISA Kit (AB314731)
  • sELISA

Supplier Data

Sandwich ELISA - Human GDF 9 ELISA Kit (AB314731)

Example of standard curve using ab314731. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 2.2 ng/mL

Range

2.2 - 500 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

Product details

Human GDF 9 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human GDF 9 in serum (human GDF 9 concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma (Collect plasma using Citrate and Heparin as an anticoagulant. EDTA is not recommended as anticoagulants for use in this assay) and cell culture supernatants. This assay employs an antibody specific for human GDF 9 coated on a 96-well plate.

Recovery

[ { "sample": "Serum", "range": "112 - 140 %", "average": "= 126.7" }, { "sample": "Plasma", "range": "77 - 147 %", "average": "= 110" }, { "sample": "Cell culture media", "range": "124 - 133 %", "average": "= 128.5" } ]

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab314731-1x96Tests", "assetComponentDetails": [ { "size":"1 x 200 µL", "name":"100X HRP-Streptavidin", "number":"AB314731-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human GDF 9 Antibody-coated ELISA Plate", "number":"AB314731-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human GDF 9 Detection Antibody", "number":"AB314731-CMP10", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B (Item E)", "number":"AB314731-CMP06", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB314731-CMP07", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB314731-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB314731-CMP08", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A (Item D)", "number":"AB314731-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human GDF 9 Protein Standard", "number":"AB314731-CMP11", "productcode":"" } ] } } }

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Product protocols

Target data

Required for ovarian folliculogenesis. Promotes primordial follicle development. Stimulates granulosa cell proliferation. Promotes cell transition from G0/G1 to S and G2/M phases, through an increase of CCND1 and CCNE1 expression, and RB1 phosphorylation. It regulates STAR expression and cAMP-dependent progesterone release in granulosa and thecal cells. Attenuates the suppressive effects of activin A on STAR expression and progesterone production by increasing the expression of inhibin B. It suppresses FST and FSTL3 production in granulosa-lutein cells.
See full target information GDF9
websiteProtocolBooklet
en

Product promise

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