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AB267609

Human GDF11 ELISA Kit

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Human GDF11 ELISA Kit is a Sandwich ELISA for the measurement of Human GDF11 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

BMP11, GDF11, Growth/differentiation factor 11, GDF-11, Bone morphogenetic protein 11, BMP-11

1 Images
Sandwich ELISA - Human GDF11 ELISA Kit (AB267609)
  • sELISA

Supplier Data

Sandwich ELISA - Human GDF11 ELISA Kit (AB267609)

This standard curve is for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture media, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.16 ng/mL

Range

0.164 - 40 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human GDF11 ELISA Kit is designed for the quantitative determination of GDF11 in cell culture supernatants, plasma and serum samples.

ΔNote: Human GDF11 concentration is low in normal serum/plasma, and may not be detectable in this assay.

This assay employs an antibody specific for human GDF11 coated on a 96-well plate. Standards and samples are pipetted into the wells and GDF11 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human GDF11 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of GDF11 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
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Recovery

[ { "sample": "Serum", "range": "111 - 122 %", "average": "= 115.7" }, { "sample": "Plasma", "range": "80 - 117 %", "average": "= 98.47" }, { "sample": "Cell culture media", "range": "83 - 97 %", "average": "= 90.79" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab267609-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB267609-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB267609-CMP05", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB267609-CMP04", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C", "number":"AB267609-CMP03", "productcode":"" }, { "size":"1 x 200 µL", "name":"700X HRP-Streptavidin Concentrate", "number":"AB267609-CMP07", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB267609-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human GDF11 Standard (Lyophilized)", "number":"AB267609-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human GDF11 coated Microplate (12 x 8 wells)", "number":"AB267609-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human GDF11 Detection Antibody", "number":"AB267609-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Secreted signal that acts globally to regulate anterior/posterior axial patterning during development. May play critical roles in patterning both mesodermal and neural tissues (By similarity). It is required for proper vertebral patterning and orofacial development (PubMed : 31215115). Signals through activin receptors type-2, ACVR2A and ACVR2B, and activin receptors type-1, ACVR1B, ACVR1C and TGFBR1 leading to the phosphorylation of SMAD2 and SMAD3 (PubMed : 28257634).
See full target information GDF11
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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