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AB100525

Human GDNF ELISA Kit (Glial Derived Neurotrophic Factor)

4

(1 Review)

|

(9 Publications)

Human GDNF ELISA Kit (Glial Derived Neurotrophic Factor) is a sandwich ELISA designed to quantify Human GDNF (Glial Derived Neurotrophic Factor) with a sensitivity of 4 pg/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 2.74 - 2000 pg/mL

View Alternative Names

Glial cell line-derived neurotrophic factor, hGDNF, Astrocyte-derived trophic factor, ATF, GDNF

3 Images
Sandwich ELISA - Human GDNF ELISA Kit (Glial Derived Neurotrophic Factor) (AB100525)
  • sELISA

Unknown

Sandwich ELISA - Human GDNF ELISA Kit (Glial Derived Neurotrophic Factor) (AB100525)

Representative standard curve using ab100525

Sandwich ELISA - Human GDNF ELISA Kit (Glial Derived Neurotrophic Factor) (AB100525)
  • sELISA

Unknown

Sandwich ELISA - Human GDNF ELISA Kit (Glial Derived Neurotrophic Factor) (AB100525)

Representative standard curve using ab100525

Sandwich ELISA - Human GDNF ELISA Kit (Glial Derived Neurotrophic Factor) (AB100525)
  • sELISA

Lab

Sandwich ELISA - Human GDNF ELISA Kit (Glial Derived Neurotrophic Factor) (AB100525)

HuGDNF measured in biological fluids showing quantity (pg) per mL of tested sample

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 4 pg/mL

Range

2.74 - 2000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human GDNF ELISA Kit (Glial Derived Neurotrophic Factor) ab100525 is a sandwich ELISA to measure Human GDNF (Glial Derived Neurotrophic Factor) in plasma, cell culture supernatant with a sensitivity of 4 pg/ml.

How the assay works

This assay employs an antibody specific for Human GDNF coated on a 96-well plate. Standards and samples are pipetted into the wells and GDNF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human GDNF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of GDNF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Human GDNF ELISA Kit (Glial Derived Neurotrophic Factor) ab100525 protocol summary

1. Add standard or sample to each well used. Incubate at room temperature
2. Wash and add prepared biotinylated antibody to each well. Incubate at room temperature.
3. Wash and add prepared streptavidin solution. Incubate at room temperature.
4. Add TMB Development Solution to each well. Incubate at room temperature.
5. Add Stop Solution to each well. Read at 450nm immediately.

Optimization may be required with urine samples

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Recovery

[ { "sample": "Cell culture supernatant", "range": "85 - 140 %", "average": "= 119.8" }, { "sample": "Plasma", "range": "86 - 113 %", "average": "= 99.25" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100525-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100525-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100525-CMP05", "productcode":"" }, { "size":"1 x 200 µL", "name":"400X HRP-Streptavidin Concentrate", "number":"AB100525-CMP02", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100525-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"GDNF Microplate (12 x 8 wells)", "number":"AB100525-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human GDNF Standard (lyophilized)", "number":"AB100525-CMP08", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100525-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human GDNF", "number":"AB100525-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100525-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake (PubMed : 8493557). Acts by binding to its coreceptor, GFRA1, leading to autophosphorylation and activation of the RET receptor (PubMed : 10829012, PubMed : 25242331, PubMed : 31535977). Involved in the development of the neural crest (PubMed : 15242795).
See full target information GDNF
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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Future science OA 11:2471723 PubMed40035430

2025

Biodistribution and persistence of human umbilical cord-derived mesenchymal stem cells in NCG mice: a comparative study.

Applications

Unspecified application

Species

Unspecified reactive species

Guangyang Liu,Herui Wang,Xin Li,Yi Mi,Chenliang Zhang,Yaoyao Chen,Li Miao,Haomiao Long,Jun He,Qinggang Ge,Yongjun Liu

Journal of personalized medicine 11: PubMed34575696

2021

Prediction of Sarcopenia Using Multiple Biomarkers of Neuromuscular Junction Degeneration in Chronic Obstructive Pulmonary Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Asima Karim,Tahir Muhammad,Rizwan Qaisar

Cell transplantation 29:963689720960185 PubMed33028107

2020

Transplantation of Adipose-Derived Stem Cells Alleviates Striatal Degeneration in a Transgenic Mouse Model for Multiple System Atrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Christine Chang,Jen-Wei Liu,Bo Cheng Chen,Zhe Sheng Jiang,Chi Tang Tu,Che Hung Su,Hsin Han Yang,Zong Qi Liu,Yu Chen Deng,Chih Yu Chen,Sheng-Tzung Tsai,Shinn Zong Lin,Tzyy-Wen Chiou

Cell transplantation 29:963689720908512 PubMed32292068

2020

GDNF Promotes Survival and Therapeutic Efficacy of Human Adipose-Derived Mesenchymal Stem Cells in a Mouse Model of Parkinson's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Shoujia Sun,Quan Zhang,Man Li,Pan Gao,Kuan Huang,Rajluxmee Beejadhursing,Wei Jiang,Ting Lei,Mingxin Zhu,Kai Shu

Endocrine practice : official journal of the Ameri 25:951-965 PubMed31170370

2019

THE ASSOCIATION OF DECREASED SERUM GDNF LEVEL WITH HYPERGLYCEMIA AND DEPRESSION IN TYPE 2 DIABETES MELLITUS.

Applications

Unspecified application

Species

Unspecified reactive species

Yanxiaoxiao Yang,Bo Xie,Changping Ju,Hui Jin,Xiuli Ye,Li Yao,Min Jia,Zilin Sun,Yonggui Yuan

Journal of sport and health science 6:447-453 PubMed30356625

2018

The effects of aerobic exercise training on oxidant-antioxidant balance, neurotrophic factor levels, and blood-brain barrier function in obese and non-obese men.

Applications

Unspecified application

Species

Unspecified reactive species

Hee-Tae Roh,Wi-Young So

PloS one 13:e0194023 PubMed29608602

2018

ER-positive breast cancer cells are poised for RET-mediated endocrine resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Sachi Horibata,Edward J Rice,Chinatsu Mukai,Brooke A Marks,Kelly Sams,Hui Zheng,Lynne J Anguish,Scott A Coonrod,Charles G Danko

Scientific reports 7:8674 PubMed28819313

2017

The protein and mRNA expression levels of glial cell line-derived neurotrophic factor in post stroke depression and major depressive disorder.

Applications

Unspecified application

Species

Unspecified reactive species

Yanran Zhang,Haitang Jiang,Yingying Yue,Yingying Yin,Yuqun Zhang,Jinfeng Liang,Shenghua Li,Jun Wang,Jianxin Lu,Deqin Geng,Aiqin Wu,Yonggui Yuan

Tissue engineering. Part A 20:3089-100 PubMed24837134

2014

Stem cells of the apical papilla regulate trigeminal neurite outgrowth and targeting through a BDNF-dependent mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Jose Flavio A de Almeida,Paul Chen,Michael A Henry,Anibal Diogenes
View all publications
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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