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Human GDNF Receptor alpha/GFRA1 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human GDNF Receptor alpha/GFRA1 in Human in Cell culture supernatant, Plasma, Serum samples.

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Images

Sandwich ELISA - Human GDNF Receptor alpha/GFRA1 ELISA Kit (AB309400), expandable thumbnail

Key facts

Detection method
Colorimetric
Sample types
Cell culture supernatant, Plasma, Serum
Assay type
Sandwich (quantitative)
Reactive species
Human
Range
0.35 - 80 ng/mL
Sensitivity
= 0.35 ng/mL

Reactivity data

Application
sELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Target data

Function

Coreceptor for GDNF, a neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake (PubMed:10829012, PubMed:31535977). GDNF-binding leads to autophosphorylation and activation of the RET receptor (PubMed:31535977).

Alternative names

GDNFRA, RETL1, TRNR1, GFRA1, GDNF family receptor alpha-1, GDNF receptor alpha-1, GDNFR-alpha-1, GFR-alpha-1, RET ligand 1, TGF-beta-related neurotrophic factor receptor 1

What's included?

1 x 96 Tests
Components
20X Wash Buffer
1 x 25 mL
5X Assay Diluent B (Item E)
1 x 15 mL
5X Assay Diluent D (Item K)
1 x 15 mL
700X HRP-Streptavidin
1 x 200 µL
Biotinylated Human GFR alpha-1/GDNF R Detection Antibody
2 x 1 Vial
Human GFR alpha-1/GDNF R Antibody-coated ELISA Plate
1 x 1 Unit
Lyophilized Human GFR alpha-1/GDNF R Protein Standard
2 x 1 Vial
Stop Solution; 0.2M Sulfuric Acid
1 x 8 mL
TMB One-Step Substrate Reagent
1 x 12 mL

Recommended products

Human GDNF Receptor alpha/GFRA1 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human GDNF Receptor alpha/GFRA1 in Human in Cell culture supernatant, Plasma, Serum samples.

Key facts

Detection method
Colorimetric
Sample types
Cell culture supernatant, Plasma, Serum
Assay type
Sandwich (quantitative)
Reactive species
Human
Range
0.35 - 80 ng/mL
Assay Platform
Pre-coated microplate (12 x 8 well strips)
Sensitivity
= 0.35 ng/mL

Recovery

Sample specific recovery

Sample type
Plasma
Average %
= 122.8
Range
106 - 143 %
Sample type
Serum
Average %
= 129.8
Range
104 - 140 %
Sample type
Cell culture media
Average %
= 93.9
Range
73 - 116 %

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

Human GDNF Receptor alpha/GFRA1 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human GDNF in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human GDNF coated on a 96-well plate.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Sandwich ELISA - Human GDNF Receptor alpha/GFRA1 ELISA Kit (ab309400), expandable thumbnail

    Sandwich ELISA - Human GDNF Receptor alpha/GFRA1 ELISA Kit (ab309400)

    Example of standard curve using ab309400. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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