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AB309400

Human GDNF Receptor alpha/GFRA1 ELISA Kit

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Human GDNF Receptor alpha/GFRA1 ELISA Kit is a Sandwich ELISA for the measurement of Human GDNF Receptor alpha/GFRA1 in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

GDNFRA, RETL1, TRNR1, GFRA1, GDNF family receptor alpha-1, GDNF receptor alpha-1, GDNFR-alpha-1, GFR-alpha-1, RET ligand 1, TGF-beta-related neurotrophic factor receptor 1

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Sandwich ELISA - Human GDNF Receptor alpha/GFRA1 ELISA Kit (AB309400)
  • sELISA

Supplier Data

Sandwich ELISA - Human GDNF Receptor alpha/GFRA1 ELISA Kit (AB309400)

Example of standard curve using ab309400. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.35 ng/mL

Range

0.35 - 80 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB8026

Anti-GDNF Receptor alpha 1/GFRA1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDNF Receptor alpha 1/GFRA1 antibody (AB8026)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human GDNF Receptor alpha/GFRA1 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human GDNF in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human GDNF coated on a 96-well plate.
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Recovery

[ { "sample": "Plasma", "range": "106 - 143 %", "average": "= 122.8" }, { "sample": "Serum", "range": "104 - 140 %", "average": "= 129.8" }, { "sample": "Cell culture media", "range": "73 - 116 %", "average": "= 93.9" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab309400-1x96Tests", "assetComponentDetails": [ { "size":"2 x 1 Vial", "name":"Lyophilized Human GFR alpha-1/GDNF R Protein Standard", "number":"AB309400-CMP11", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent D (Item K)", "number":"AB309400-CMP06", "productcode":"" }, { "size":"1 x 200 µL", "name":"700X HRP-Streptavidin", "number":"AB309400-CMP12", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B (Item E)", "number":"AB309400-CMP05", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB309400-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB309400-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human GFR alpha-1/GDNF R Antibody-coated ELISA Plate", "number":"AB309400-CMP09", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB309400-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human GFR alpha-1/GDNF R Detection Antibody", "number":"AB309400-CMP10", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Coreceptor for GDNF, a neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake (PubMed : 10829012, PubMed : 31535977). GDNF-binding leads to autophosphorylation and activation of the RET receptor (PubMed : 31535977).
See full target information GFRA1
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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