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AB270215

Human Gelsolin ELISA Kit

5

(1 Review)

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(6 Publications)

Human Gelsolin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Gelsolin with a sensitivity of 145.98 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations

View Alternative Names

Gelsolin, AGEL, Actin-depolymerizing factor, Brevin, ADF, GSN

3 Images
ELISA - Human Gelsolin ELISA Kit (AB270215)
  • ELISA

Supplier Data

ELISA - Human Gelsolin ELISA Kit (AB270215)
Sandwich ELISA - Human Gelsolin ELISA Kit (AB270215)
  • sELISA

Supplier Data

Sandwich ELISA - Human Gelsolin ELISA Kit (AB270215)

Interpolated concentrations of native Gelsolin in human serum and plasma samples.

The concentrations of Gelsolin were measured in duplicates, interpolated from the Gelsolin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 1000, plasma (citrate) 1 : 1000, plasma (EDTA) 1 : 1000, and plasma (Heparin) 1 : 2000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Gelsolin concentration was determined to be 12.54 μg/mL in serum, 18.80 μg/mL in plasma (citrate), 8.41 μg/mL in plasma (EDTA), and 48.98 μg/mL in plasma (Heparin).

Sandwich ELISA - Human Gelsolin ELISA Kit (AB270215)
  • sELISA

Supplier Data

Sandwich ELISA - Human Gelsolin ELISA Kit (AB270215)

Example of human Gelsolin standard curve in Sample Diluent NS.

The Gelsolin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 145.98 pg/mL

Range

546.88 - 35000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Gelsolin ELISA Kit ab270215 is a rapid single-wash 90-min Sandwich ELISA to measure Human Gelsolin in citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 145.98 pg/mL.

How the assay works

Human Gelsolin SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Gelsolin ELISA Kit ab270215 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Gelsolin antibody [EPR22482-78] - BSA and Azide free (Capture) ab272297
- Anti-Gelsolin antibody [EPR22482-236] - BSA and Azide free (Detector) ab272297

Gelsolin (GSN) is an 82-kD protein with six homologous subdomains, referred to as S1-S6. Each subdomain is composed of a five-stranded ?-sheet, flanked by two ?-helices, one positioned perpendicular with respect to the strands and one positioned parallel. The ?-sheets of the three N-terminal subdomains (S1-S3) join to form an extended ?-sheet, as do the ?-sheets of the C-terminal subdomains (S4-S6). It is regulated by calcium and modulates actin by binding to the plus (or barbed) ends of actin filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Gelsolin plays a role in ciliogenesis. Human Gelsolin shares 99%, 93%, 93%, and 96% sequence homology with monkey, mouse, rat, and bovine, respectively.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.2" } ]
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Recovery

[ { "sample": "Serum", "range": "94 - 114 %", "average": "= 104" }, { "sample": "EDTA Plasma", "range": "92 - 97 %", "average": "= 94" }, { "sample": "Heparin Plasma", "range": "87 - 92 %", "average": "= 90" }, { "sample": "Citrate plasma", "range": "81 - 94 %", "average": "= 90" } ]
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What's included?

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab270215-10x96Tests", "assetComponentDetails": [ { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB270215-CMP06" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB270215-CMP10" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB270215-CMP07" }, { "size":"2 x 250 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB270215-CMP04" }, { "size":"10 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB270215-CMP03" }, { "size":"2 x 10 Vial", "name":"Human Gelsolin Lyophilized Recombinant Protein", "number":"AB270215-CMP09" }, { "size":"1 x 6000 µL", "name":"10X Human Gelsolin Capture Antibody", "number":"AB270215-CMP01" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB270215-CMP05" }, { "size":"1 x 6000 µL", "name":"10X Human Gelsolin Detector Antibody", "number":"AB270215-CMP02" }, { "size":"10 x 1 Unit", "name":"Plate Seals", "number":"AB270215-CMP08" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab270215-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB270215-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB270215-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB270215-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB270215-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB270215-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Gelsolin Lyophilized Recombinant Protein", "number":"AB270215-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Gelsolin Capture Antibody", "number":"AB270215-CMP01", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB270215-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Gelsolin Detector Antibody", "number":"AB270215-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB270215-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Gelsolin is a multifunctional actin-binding protein that exists in two main isoforms: cytoplasmic and plasma. It has a molecular mass of approximately 82 kDa. Gelsolin is expressed in a wide range of tissues including muscle epithelial and nerve cells. The protein's main mechanical function involves severing actin filaments which helps to regulate actin polymerization and depolymerization. Researchers sometimes refer to gelsolin as 'GSN'. The presence of the protein helps maintain the dynamic organization of the cytoskeleton by interacting with actin filaments.
Biological function summary

This protein acts in cell movement apoptosis and signal transduction. Gelsolin contributes to the remodeling of the actin cytoskeleton by promoting the disassembly of actin filaments allowing cells to change shape and move. Although it operates independently gelsolin’s activity can be influenced by calcium ions and phosphoinositides leading to its participation in various cellular activities. Gelsolin plays a role in platelet activation and may regulate cellular responses through association with the cytoskeletal complex.

Pathways

Gelsolin participates in the regulation of the actin cytoskeleton and apoptotic pathways. Within these pathways gelsolin interacts with proteins like cofilin to modulate actin filament dynamics. In apoptotic pathways it collaborates by altering the cytoskeletal architecture impacting cell death mechanisms. Gelsolin modifications in these pathways have implications for cellular processes like cell migration and apoptosis bringing it into relation with proteins such as caspases.

Gelsolin is linked to conditions such as gelsolin amyloidosis and cancer metastasis. Gelsolin amyloidosis results from mutations in the gelsolin gene which lead to the formation of amyloid fibrils causing dysfunction in nerves eyes and skin. In cancer altered gelsolin expression can influence tumor progression and metastasis through its role in cell motility. Related proteins such as actin and caspases could also play a part in these conditions either through direct interaction or through pathways in which gelsolin functions.
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Product protocols

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Target data

Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed (PubMed : 19666512). Plays a role in ciliogenesis (PubMed : 20393563).
See full target information GSN
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

JCI insight 9: PubMed39352744

2024

Interpretable machine learning uncovers epithelial transcriptional rewiring and a role for Gelsolin in COPD.

Applications

Unspecified application

Species

Unspecified reactive species

Justin Sui,Hanxi Xiao,Ugonna Mbaekwe,Nai-Chun Ting,Kaley Murday,Qianjiang Hu,Alyssa D Gregory,Theodore S Kapellos,Ali Öender Yildirim,Melanie Königshoff,Yingze Zhang,Frank Sciurba,Jishnu Das,Corrine R Kliment

Hepatology communications 8: PubMed38829196

2024

Proteomic analysis of serum extracellular vesicles reveals Fibulin-3 as a new marker predicting liver-related events in MASLD.

Applications

Unspecified application

Species

Unspecified reactive species

Sadatsugu Sakane,Hayato Hikita,Kumiko Shirai,Tatsuya Sakamoto,Ryohei Narumi,Jun Adachi,Naruyasu Kakita,Yukinori Yamada,Hidenori Toyoda,Hirokazu Takahashi,Goki Suda,Machiko Kai,Yuki Tahata,Ryotaro Sakamori,Shusuke Kumazaki,Kenji Fukumoto,Yuta Myojin,Kazuhiro Murai,Takahiro Kodama,Tomohide Tatsumi,Takeshi Tomonaga,Naoya Sakamoto,Eiichi Morii,Tetsuo Takehara

iScience 27:108930 PubMed38333700

2024

Serum proteome profiling reveals heparanase as a candidate biomarker for chronic thromboembolic pulmonary hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Yunxia Zhang,Meng Zhang,Hongwei Yang,Haobo Li,Shuangshuang Ma,Linfeng Xi,Yishan Li,Xincheng Li,Zhihui Fu,Zhu Zhang,Shuai Zhang,Qian Gao,Qiang Huang,Jun Wan,Wanmu Xie,Jifeng Li,Peiran Yang,Zhenguo Zhai

Frontiers in genetics 14:1093163 PubMed37035750

2023

Gelsolin: A comprehensive pan-cancer analysis of potential prognosis, diagnostic, and immune biomarkers.

Applications

Unspecified application

Species

Unspecified reactive species

Yiyang Wang,Xiaojuan Bi,Zhiwen Luo,Haiyan Wang,Dilimulati Ismtula,Chenming Guo

Journal of neurology, neurosurgery, and psychiatry 94:436-447 PubMed36650038

2023

gene frameshift mutations in Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Yaling Jiang,Meidan Wan,XueWen Xiao,Zhuojie Lin,Xixi Liu,Yafang Zhou,Xinxin Liao,Jingyi Lin,Hui Zhou,Lu Zhou,Ling Weng,Junling Wang,Jifeng Guo,Hong Jiang,Zhuohua Zhang,Kun Xia,Jiada Li,Beisha Tang,Bin Jiao,Lu Shen

International journal of molecular sciences 22: PubMed33920336

2021

Exosomal Aβ-Binding Proteins Identified by "In Silico" Analysis Represent Putative Blood-Derived Biomarker Candidates for Alzheimer´s Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Tânia Soares Martins,Rui Marçalo,Maria Ferreira,Margarida Vaz,Raquel M Silva,Ilka Martins Rosa,Jonathan Vogelgsang,Jens Wiltfang,Odete A B da Cruz E Silva,Ana Gabriela Henriques
View all publications
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