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AB223867

Human GFAP ELISA Kit

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(3 Publications)

Human GFAP ELISA Kit is a single-wash 90-min Simplestep used to quantify Human GFAP with a sensitivity of 47 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Glial fibrillary acidic protein, GFAP

3 Images
ELISA - Human GFAP ELISA Kit (AB223867)
  • ELISA

Supplier Data

ELISA - Human GFAP ELISA Kit (AB223867)
Sandwich ELISA - Human GFAP ELISA Kit (AB223867)
  • sELISA

Supplier Data

Sandwich ELISA - Human GFAP ELISA Kit (AB223867)

Example of human GFAP standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human GFAP ELISA Kit (AB223867)
  • sELISA

Supplier Data

Sandwich ELISA - Human GFAP ELISA Kit (AB223867)

Interpolated concentrations of native GFAP in Human brain extract based on a 100 ng/mL extract load.

The concentrations of GFAP were measured in duplicate and interpolated from the GFAP standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean GFAP concentration was determined to be 46.27 ng/mL per 100 ng/ml extract load.

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 47 pg/mL

Range

0.781 - 50 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human GFAP ELISA Kit ab223867 is a rapid single-wash 90-min Sandwich ELISA to measure Human GFAP in tissue extracts. This SimpleStep sensitivity is 47 pg/mL.

How the assay works

Human GFAP SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human GFAP ELISA Kit ab223867 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-GFAP antibody [EPR19996-132] - BSA and Azide free (Capture) ab242692
- Anti-GFAP antibody [EPR19996] - BSA and Azide free (Detector) ab242937

Glial fibrillary acidic protein (GFAP) is a class-III intermediate filament, cell-specific marker that during development of the central nervous system, distinguishes astrocytes from other glial cells. Protein expression is also found in numerous cell types including astrocytes, ependymal cells, osteocytes, keratinocytes and chondrocytes. GFAP has also been found to be expressed in rat glomeruli, peritubular fibroblasts, stellate cells of the pancreas and liver tissue as well as Leydig cells of the testis in both hamsters and humans.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Brain Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "11.8" }, { "reproducibilityType": "Intra", "sample": "Brain Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.3" } ]
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Recovery

[ { "sample": "Tissue Extracts", "range": "116 - 119 %", "average": "= 117" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab223867-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB223867-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB223867-CMP13", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CP2", "number":"AB223867-CMP14", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human GFAP Capture Antibody", "number":"AB223867-CMP09", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB223867-CMP12", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human GFAP Lyophilized Recombinant Protein", "number":"AB223867-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB223867-CMP05", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB223867-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human GFAP Detector Antibody", "number":"AB223867-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB223867-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB223867-CMP08", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB223867-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
See full target information GFAP
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:14236 PubMed38902461

2024

Electrical impedance tomography provides information of brain injury during total aortic arch replacement through its correlation with relative difference of neurological biomarkers.

Applications

Unspecified application

Species

Unspecified reactive species

Yitong Guo,Chen Yang,Wenjing Zhu,Rong Zhao,Kai Ren,Weixun Duan,Jincheng Liu,Jing Ma,Xiuming Chen,Benyuan Liu,Canhua Xu,Zhenxiao Jin,Xuetao Shi

BMC anesthesiology 21:182 PubMed34182951

2021

Effects of intraoperative lung-protective ventilation on clinical outcomes in patients with traumatic brain injury: a randomized controlled trial.

Applications

Unspecified application

Species

Unspecified reactive species

Lulu Jiang,Yujuan Wu,Yang Zhang,Dahao Lu,Keshi Yan,Ju Gao

Journal of neurosurgery :1-8 PubMed32707544

2020

Plasma extracellular vesicles as a source of biomarkers in traumatic brain injury.

Applications

Unspecified application

Species

Unspecified reactive species

Ross C Puffer,Luz M Cumba Garcia,Benjamin T Himes,Mi-Yeon Jung,Frederic B Meyer,David O Okonkwo,Ian F Parney
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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