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AB288175

Human GFAP ELISA Kit

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Human GFAP ELISA Kit is a single-wash 90-min Simplestep used to quantify Human GFAP with a sensitivity of 0.068 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Validated on a number of sample types including cerebrospinal fluid (CSF)
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Glial fibrillary acidic protein, GFAP

3 Images
ELISA - Human GFAP ELISA Kit (AB288175)
  • ELISA

Supplier Data

ELISA - Human GFAP ELISA Kit (AB288175)
Sandwich ELISA - Human GFAP ELISA Kit (AB288175)
  • sELISA

Supplier Data

Sandwich ELISA - Human GFAP ELISA Kit (AB288175)

Interpolated concentrations of human GFAP in human cerebrospinal fluid. Interpolated concentration of native GFAP was measured in duplicate at different sample concentrations. Undiluted samples are 100% cerebrospinal fluid. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human GFAP ELISA Kit (AB288175)
  • sELISA

Supplier Data

Sandwich ELISA - Human GFAP ELISA Kit (AB288175)

Example of human GFAP standard curve in Sample Diluent NS. Example of human GFAP standard curve. Background-subtracted data values (mean +/- SD)

Key facts

Detection method

Colorimetric

Sample types

Cerebral Spinal Fluid, Cell culture media

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.068 ng/mL

Range

0.625 - 40 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Product details

Human GFAP ELISA Kit ab288175 is a rapid single-wash 90-min Sandwich ELISA to measure Human GFAP in cell culture media, CSF. This SimpleStep sensitivity is 0.068 ng/mL.

How the assay works

Human GFAP SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human GFAP ELISA Kit ab288175 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-GFAP antibody [EPR19996-132] - BSA and Azide free (Capture) ab242937
- Anti-GFAP antibody [EPR19996] - BSA and Azide free (Detector) ab242937
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Precision

[ { "reproducibilityType": "Inter", "sample": "Cerebral Spinal Fluid", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "12.4" }, { "reproducibilityType": "Intra", "sample": "Cerebral Spinal Fluid", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "11.1" } ]
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Recovery

[ { "sample": "Cerebral Spinal Fluid", "range": "108 - 111 %", "average": "= 109" }, { "sample": "Cell culture media", "range": "100 - 104 %", "average": "= 102" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab288175-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB288175-CMP04", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CP2", "number":"AB288175-CMP12", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB288175-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human GFAP Capture Antibody", "number":"AB288175-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB288175-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human GFAP Lyophilized Recombinant Protein", "number":"AB288175-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB288175-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB288175-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB288175-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human GFAP Detector Antibody", "number":"AB288175-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
See full target information GFAP
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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websiteProtocolBooklet
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com