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Human GFAP ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human GFAP in Cerebral Spinal Fluid, Cell culture media samples.

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Images

Sandwich ELISA - Human GFAP ELISA Kit (AB288175), expandable thumbnail
  • Sandwich ELISA - Human GFAP ELISA Kit (AB288175), expandable thumbnail

Key facts

Detection method

Colorimetric

Sample types

Cerebral Spinal Fluid, Cell culture media

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

0.625 - 40 ng/mL

Assay time

1h 30m

Sensitivity

= 0.068 ng/mL

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Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info
-
Notes

-

Target data

Function

GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.

Alternative names

What's included?

1 x 96 Tests
Components
10X Human GFAP Capture Antibody
1 x 600 µL
10X Human GFAP Detector Antibody
1 x 600 µL
10X Wash Buffer PT (ab206977)
1 x 20 mL
Antibody Diluent CP2
1 x 6 mL
Human GFAP Lyophilized Recombinant Protein
2 x 1 Vial
Plate Seals
1 x 1 Unit
Sample Diluent NS (ab193972)
1 x 12 mL
SimpleStep Pre-Coated 96-Well Microplate (ab206978)
1 x 1 Unit
Stop Solution
1 x 12 mL
TMB Development Solution
1 x 12 mL

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Human GFAP ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human GFAP in Cerebral Spinal Fluid, Cell culture media samples.

Key facts

Detection method

Colorimetric

Sample types

Cerebral Spinal Fluid, Cell culture media

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

0.625 - 40 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Sensitivity

= 0.068 ng/mL

Precision

Intra assay

Sample

Cerebral Spinal Fluid

n
8
mean
-
SD
-
C.V.

11.1

Inter assay

Sample

Cerebral Spinal Fluid

n
3
mean
-
SD
-
C.V.

12.4

Recovery

Sample specific recovery

Sample type

Cerebral Spinal Fluid

Average %

= 109

Range

108 - 111 %

Sample type

Cell culture media

Average %

= 102

Range

100 - 104 %

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Human GFAP ELISA Kit (ab288175) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human GFAP protein in cell culture media and cerebrospinal fluid samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human GFAP with 0.068 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Browse our curated portfolio of extensively validated antibodies and assays, with antibodies to over 90% of key targets in major glioblastoma pathways– including EGF, VEGF, MAPK, Wnt, NFKB, and PI3K/AkT/MTOR – all in one place.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Sandwich ELISA - Human GFAP ELISA Kit (ab288175), expandable thumbnail

    Sandwich ELISA - Human GFAP ELISA Kit (ab288175)

    Interpolated concentrations of human GFAP in human cerebrospinal fluid. Interpolated concentration of native GFAP was measured in duplicate at different sample concentrations. Undiluted samples are 100% cerebrospinal fluid. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

  • Sandwich ELISA - Human GFAP ELISA Kit (ab288175), expandable thumbnail

    Sandwich ELISA - Human GFAP ELISA Kit (ab288175)

    Example of human GFAP standard curve in Sample Diluent NS. Example of human GFAP standard curve. Background-subtracted data values (mean +/- SD)

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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