Human GHR ELISA Kit (Growth Hormone Receptor)

Product details

Human GHR ELISA Kit (Growth Hormone Receptor) (ab260060) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of GHR (Growth Hormone Receptor) protein in cit plasma, edta plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human GHR (Growth Hormone Receptor) with 69.68 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Human Growth Hormone Receptor (GHR) is a receptor protein that binds pituitary gland growth hormone involved in regulating postnatal body growth. Human GHR cDNA encodes a 638 amino acid (aa) residue type I transmembrane protein with an 18 aa putative signal peptide, a 246 aa extracellular domain, a 24 aa transmembrane domain and a 350 aa cytoplasmic domain. At least two alternatively spliced isoforms of human GHR, lacking the sequence encoded by exon 3, or lacking most of the cytoplasmic domain, also exist. Soluble GH-binding proteins corresponding to extracellular domain of the transmembrane proteins can be generated from the membrane proteins. Ligation of GHR by GH results in receptor dimerization and activation of the JAK/STAT signaling cascade. The soluble GHBP has been shown to interfere with GH signaling by competing with the transmembrane receptor of GH. Alternatively, the GHBP has also been shown to enhance GH action by slowing GH clearance. Human GHR shares 66%, 68%, 67%, and 94% sequence homology with mouse, rat, cow, and monkey respectively.