AB260060

Human GHR ELISA Kit (Growth Hormone Receptor)

Recombinant
SimpleStep
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Human GHR ELISA Kit (Growth Hormone Receptor) is a single-wash 90-min Simplestep used to quantify Human GHR (Growth Hormone Receptor) with a sensitivity of 69.68 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Growth hormone receptor, GH receptor, Somatotropin receptor, GHR

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Sandwich ELISA - Human GHR ELISA Kit (Growth Hormone Receptor) (AB260060)

sELISA

Supplier Data

Sandwich ELISA - Human GHR ELISA Kit (Growth Hormone Receptor) (AB260060)

Interpolated concentrations of native GHR in human serum and plasma samples.

The concentrations of GHR were measured in duplicates, interpolated from the GHR standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 25%, plasma (citrate) 25%, and plasma (EDTA) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean GHR concentration was determined to be 31.76 ng/mL in serum, 31.76 ng/mL in plasma (citrate), and 21.28 ng/mL in plasma (EDTA).

sELISA

Supplier Data

Sandwich ELISA - Human GHR ELISA Kit (Growth Hormone Receptor) (AB260060)

Example of human GHR standard curve in Sample Diluent NS.

The GHR standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 69.68 pg/mL

Range

156.25 - 10000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human GHR ELISA Kit (Growth Hormone Receptor) (ab260060) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of GHR (Growth Hormone Receptor) protein in cit plasma, edta plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human GHR (Growth Hormone Receptor) with 69.68 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.7" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.8" } ]

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Recovery

[ { "sample": "Serum", "range": "97 - 112 %", "average": "= 106" }, { "sample": "EDTA Plasma", "range": "107 - 121 %", "average": "= 115" }, { "sample": "Citrate plasma", "range": "100 - 103 %", "average": "= 101" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Growth Hormone Receptor (GHR) also known as somatotropin receptor is a vital protein that mediates the effects of growth hormone (GH). GHR is a transmembrane receptor with a mass of approximately 70 kDa and is expressed in many tissues including liver muscle and adipose tissue. It plays an important role in growth regulation by binding to growth hormone which triggers signal transduction pathways involved in cellular proliferation and growth.

Biological function summary

The growth hormone receptor facilitates the hormone's ability to promote growth and cell differentiation. The receptor when activated stimulates the JAK2/STAT5 signaling pathway a part of a larger GHR complex. This complex leads to gene transcription that stimulates growth and metabolism. The activation of GHR has significant effects on the cellular functions related to protein synthesis lipid metabolism and glucose metabolism.

Pathways

The growth hormone receptor is an important component of the JAK-STAT signaling pathway and the mTOR signaling pathway. In these pathways GHR interacts with other proteins such as Janus kinase 2 (JAK2) and signal transducer and activator of transcription 5 (STAT5). These interactions play a critical role in translating the binding of growth hormone into cellular responses that lead to growth and metabolic regulation.

Disruptions or mutations in the growth hormone receptor can lead to conditions such as Laron syndrome and growth hormone insensitivity. These conditions often display symptoms of dwarfism and metabolic aberrations due to impaired signaling. GHR malfunction relates closely to growth hormone (GH) and anti-growth hormone treatments like somatropin used for managing growth deficiencies. The connection of GHR with related pathways and proteins like the IGF-1 (Insulin-like Growth Factor 1) underlines its role in metabolic diseases.

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Product protocols

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Target data

Receptor for pituitary gland growth hormone (GH1) involved in regulating postnatal body growth (PubMed : 1549776, PubMed : 2825030, PubMed : 8943276). On ligand binding, couples to the JAK2/STAT5 pathway (PubMed : 1549776, PubMed : 15690087, PubMed : 2825030, PubMed : 8943276).. Growth hormone-binding protein. The soluble form (GHBP) acts as a reservoir of growth hormone in plasma and may be a modulator/inhibitor of GH signaling.. Isoform 2. Up-regulates the production of the soluble Growth hormone-binding protein form (GHBP) and acts as a negative inhibitor of growth hormone signaling.

See full target information GHR

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

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Associated Products

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Alternative Version

Assay Kits

AB267684

Human GHR (Growth Hormone Receptor) Antibody Pair - BSA and Azide free

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human-ghr-growth-hormone-receptor-antibody-pair-bsa-and-azide-free-ab267684

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