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AB263887

Human Ghrelin ELISA Kit (Ghrelin-28)

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Human Ghrelin ELISA Kit (Ghrelin-28) is a single-wash 90-min Simplestep used to quantify Human Ghrelin (Ghrelin-28) with a sensitivity of 1.048 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

MTLRP, UNQ524/PRO1066, GHRL, Appetite-regulating hormone, Growth hormone secretagogue, Growth hormone-releasing peptide, Motilin-related peptide, Protein M46

4 Images
Sandwich ELISA - Human Ghrelin ELISA Kit (Ghrelin-28) (AB263887)
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Supplier Data

Sandwich ELISA - Human Ghrelin ELISA Kit (Ghrelin-28) (AB263887)

Interpolated concentrations of native Ghrelin in human serum and plasma samples.

The concentrations of Ghrelin were measured in duplicates, interpolated from the Ghrelin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Ghrelin concentration was determined to be 25.69 pg/mL in neat serum and 31.66 pg/mL in neat plasma (citrate).

Sandwich ELISA - Human Ghrelin ELISA Kit (Ghrelin-28) (AB263887)
  • sELISA

Supplier Data

Sandwich ELISA - Human Ghrelin ELISA Kit (Ghrelin-28) (AB263887)

Example of human Ghrelin standard curve in Sample Diluent NS.

The Ghrelin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Ghrelin ELISA Kit (Ghrelin-28) (AB263887)
  • sELISA

Supplier Data

Sandwich ELISA - Human Ghrelin ELISA Kit (Ghrelin-28) (AB263887)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Ghrelin concentration of detectable samples was determined to be 13.42 pg/mL with a range of undetectable – 36.06 pg/mL in all samples.

Sandwich ELISA - Human Ghrelin ELISA Kit (Ghrelin-28) (AB263887)
  • sELISA

Supplier Data

Sandwich ELISA - Human Ghrelin ELISA Kit (Ghrelin-28) (AB263887)

Reactivity comparison of Ghrelin-28 (acylated peptide) and [Des-octanoyl]-Ghrelin-28.

[Des-octanoyl]-Ghrelin-28 is the non-acylated major circulating isoform of Ghrelin.

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Heparin Plasma, Citrate plasma, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 1.048 pg/mL

Range

1.95 - 125 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Ghrelin ELISA Kit (Ghrelin-28) (ab263887) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Ghrelin (Ghrelin-28) protein in cell culture media, cit plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Ghrelin (Ghrelin-28) with 1.048 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Our broad range of gold-standard reagents features highly specific recombinant antibodies and kits, validated across applications and species to ensure the precision and reproducibility your work requires. Drive your drug discovery efforts forward with our tailored solutions designed to meet the unique challenges of obesity research.

Ghrelin is a peptide hormone, it is a ligand of growth hormone secretagogue receptor type 1 (GHSR). It is involved in appetite stimulation and gastric acid secretion and induces adiposity. It is involved in growth regulation and induces the release of growth hormone from the pituitary. Ghrelin exists in two forms, Ghrelin-28 and one amino acid shorter Ghrelin-27. Both forms as well as Obestatin are generated by cleavage from common precursor protein, Appetite-Regulating Hormone coded by GHRL gene. Ghrelin-28 and Obestatin have non-overlapping amino acid sequences. O-octanoylation or O-decanoylation of Ghrelins is essential for Ghrelin activity.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.1" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.6" } ]
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Recovery

[ { "sample": "Serum", "range": "97 - 105 %", "average": "= 101" }, { "sample": "Cell culture media", "range": "96 - 99 %", "average": "= 97" }, { "sample": "Heparin Plasma", "range": "96 - 101 %", "average": "= 99" }, { "sample": "Citrate plasma", "range": "73 - 85 %", "average": "= 78" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ghrelin also known as the "hunger hormone" or GH secretagogue is a peptide hormone with a mass of 28 amino acids. Ghrelin is mainly expressed in the stomach particularly in the enteroendocrine cells but it can also be found in small amounts in the lungs pancreas and hypothalamus. The hormone increases food intake and promotes fat storage by signaling the brain to stimulate hunger. Its secretion occurs in response to fasting and is suppressed by food intake reflecting its role in energy balance.
Biological function summary

Ghrelin influences a variety of physiological processes beyond hunger regulation. It plays a role in modulating the release of growth hormone by acting on the growth hormone secretagogue receptor (GHS-R). Ghrelin also affects gastric motility and acid secretion due to its expression in the gastrointestinal tract. Though not part of a larger complex its interaction with GHS-R strongly influences its biological functions. Ghrelin may also impact insulin secretion and sensitivity linking it to glucose metabolism processes.

Pathways

Ghrelin interacts with several critical biological networks. Ghrelin is involved in the neuroendocrine pathway that connects the gut to the brain and contributes significantly to energy homeostasis. Within this pathway it influences proteins like leptin which exerts opposite effects by suppressing hunger. Additionally ghrelin functions in the hedonic control of eating which relates to the rewarding aspects of food intake controlled by pathways involving dopamine.

Ghrelin holds relevance to obesity and cachexia a form of extreme weight loss often seen in chronic illness. Ghrelin promotes adiposity by stimulating appetite which can contribute to obesity. Conversely in cachexia the decrease in ghrelin activity is thought to exacerbate weight loss. Its role in energy balance associates ghrelin with the protein leptin which counterbalances its effects and connections to metabolic disorders. Understanding ghrelin’s functions and pathways provides insight into therapeutic strategies for these conditions.
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Product protocols

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Target data

Ghrelin-27. Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR) (PubMed : 10604470). Induces the release of growth hormone from the pituitary (PubMed : 10604470). Has an appetite-stimulating effect, induces adiposity and stimulates gastric acid secretion. Involved in growth regulation.. Ghrelin-28. Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR) (PubMed : 10604470). Induces the release of growth hormone from the pituitary (PubMed : 10604470). Has an appetite-stimulating effect, induces adiposity and stimulates gastric acid secretion. Involved in growth regulation.. Obestatin. May be the ligand for GPR39. May have an appetite-reducing effect resulting in decreased food intake. May reduce gastric emptying activity and jejunal motility (By similarity).
See full target information GHRL
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

PloS one 17:e0271793 PubMed35862413

2022

A comprehensive approach to predicting weight gain and therapy response in psychopharmacologically treated major depressed patients: A cohort study protocol.

Applications

Unspecified application

Species

Unspecified reactive species

Maria S Simon,Barbara B Barton,Catherine Glocker,Richard Musil
View all publications
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websiteProtocolBooklet
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