Human Ghrelin ELISA Kit (Ghrelin-28) is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Ghrelin (Ghrelin-28) in Cell culture media, Heparin Plasma, Citrate plasma, Serum samples.
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- PloS one 17:e02717932022A comprehensive approach to predicting weight gain and therapy response in psychopharmacologically treated major depressed patients: A cohort study protocol.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaria S Simon,Barbara B Barton,Catherine Glocker,Richard MusilPubMed 35862413
Key facts
- Detection method
Colorimetric
- Sample types
Cell culture media, Heparin Plasma, Citrate plasma, Serum
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
1.95 - 125 pg/mL
- Assay time
1h 30m
- Sensitivity
= 1.048 pg/mL
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Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR). Induces the release of growth hormone from the pituitary. Has an appetite-stimulating effect, induces adiposity and stimulates gastric acid secretion. Involved in growth regulation.Obestatin may be the ligand for GPR39. May have an appetite-reducing effect resulting in decreased food intake. May reduce gastric emptying activity and jejunal motility (By similarity).
Alternative names
Appetite-regulating hormone, Growth hormone secretagogue, Growth hormone-releasing peptide, Motilin-related peptide, Protein M46, UNQ524/PRO1066, GHRL, MTLRP
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Recommended products
Human Ghrelin ELISA Kit (Ghrelin-28) is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Ghrelin (Ghrelin-28) in Cell culture media, Heparin Plasma, Citrate plasma, Serum samples.
Key facts
- Detection method
Colorimetric
- Sample types
Cell culture media, Heparin Plasma, Citrate plasma, Serum
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
1.95 - 125 pg/mL
- Assay time
1h 30m
- Assay Platform
Pre-coated microplate (12 x 8 well strips)
- Sensitivity
= 1.048 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 5.6 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 6.1 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 101 | Range 97 - 105 % |
Sample type Cell culture media | Average % = 97 | Range 96 - 99 % |
Sample type Heparin Plasma | Average % = 99 | Range 96 - 101 % |
Sample type Citrate plasma | Average % = 78 | Range 73 - 85 % |
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Human Ghrelin ELISA Kit (Ghrelin-28) (ab263887) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Ghrelin (Ghrelin-28) protein in cell culture media, cit plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Ghrelin (Ghrelin-28) with 1.048 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Ghrelin is a peptide hormone, it is a ligand of growth hormone secretagogue receptor type 1 (GHSR). It is involved in appetite stimulation and gastric acid secretion and induces adiposity. It is involved in growth regulation and induces the release of growth hormone from the pituitary. Ghrelin exists in two forms, Ghrelin-28 and one amino acid shorter Ghrelin-27. Both forms as well as Obestatin are generated by cleavage from common precursor protein, Appetite-Regulating Hormone coded by GHRL gene. Ghrelin-28 and Obestatin have non-overlapping amino acid sequences. O-octanoylation or O-decanoylation of Ghrelins is essential for Ghrelin activity.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Ghrelin also known as the "hunger hormone" or GH secretagogue is a peptide hormone with a mass of 28 amino acids. Ghrelin is mainly expressed in the stomach particularly in the enteroendocrine cells but it can also be found in small amounts in the lungs pancreas and hypothalamus. The hormone increases food intake and promotes fat storage by signaling the brain to stimulate hunger. Its secretion occurs in response to fasting and is suppressed by food intake reflecting its role in energy balance.
- Biological function summary
Ghrelin influences a variety of physiological processes beyond hunger regulation. It plays a role in modulating the release of growth hormone by acting on the growth hormone secretagogue receptor (GHS-R). Ghrelin also affects gastric motility and acid secretion due to its expression in the gastrointestinal tract. Though not part of a larger complex its interaction with GHS-R strongly influences its biological functions. Ghrelin may also impact insulin secretion and sensitivity linking it to glucose metabolism processes.
- Pathways
Ghrelin interacts with several critical biological networks. Ghrelin is involved in the neuroendocrine pathway that connects the gut to the brain and contributes significantly to energy homeostasis. Within this pathway it influences proteins like leptin which exerts opposite effects by suppressing hunger. Additionally ghrelin functions in the hedonic control of eating which relates to the rewarding aspects of food intake controlled by pathways involving dopamine.
- Associated diseases and disorders
Ghrelin holds relevance to obesity and cachexia a form of extreme weight loss often seen in chronic illness. Ghrelin promotes adiposity by stimulating appetite which can contribute to obesity. Conversely in cachexia the decrease in ghrelin activity is thought to exacerbate weight loss. Its role in energy balance associates ghrelin with the protein leptin which counterbalances its effects and connections to metabolic disorders. Understanding ghrelin’s functions and pathways provides insight into therapeutic strategies for these conditions.
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4 product images
Sandwich ELISA - Human Ghrelin ELISA Kit (Ghrelin-28) (ab263887)
Example of human Ghrelin standard curve in Sample Diluent NS.
The Ghrelin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Ghrelin ELISA Kit (Ghrelin-28) (ab263887)
Interpolated concentrations of native Ghrelin in human serum and plasma samples.
The concentrations of Ghrelin were measured in duplicates, interpolated from the Ghrelin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Ghrelin concentration was determined to be 25.69 pg/mL in neat serum and 31.66 pg/mL in neat plasma (citrate).
Sandwich ELISA - Human Ghrelin ELISA Kit (Ghrelin-28) (ab263887)
Serum from ten individual healthy human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Ghrelin concentration of detectable samples was determined to be 13.42 pg/mL with a range of undetectable – 36.06 pg/mL in all samples.
Sandwich ELISA - Human Ghrelin ELISA Kit (Ghrelin-28) (ab263887)
Reactivity comparison of Ghrelin-28 (acylated peptide) and [Des-octanoyl]-Ghrelin-28.
[Des-octanoyl]-Ghrelin-28 is the non-acylated major circulating isoform of Ghrelin.
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