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AB313516

Human GIP ELISA Kit

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Human GIP ELISA Kit is a Sandwich ELISA for the measurement of Human GIP in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

CTRP1, UNQ310/PRO353, C1QTNF1, Complement C1q tumor necrosis factor-related protein 1, G protein-coupled receptor-interacting protein, GIP

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Sandwich ELISA - Human GIP ELISA Kit (AB313516)
  • sELISA

Supplier Data

Sandwich ELISA - Human GIP ELISA Kit (AB313516)

Example of standard curve using ab313516. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.61 ng/mL

Range

0.61 - 150 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB25973

Anti-GIP antibody

Immunocytochemistry/ Immunofluorescence - Anti-GIP antibody (AB25973)

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  • 1
Proteins & Peptides

AB151376

Recombinant Human GIP protein

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  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human GIP ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human GIP in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human GIP coated on a 96-well plate.
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Recovery

[ { "sample": "Cell culture media", "range": "73 - 81 %", "average": "= 79.13" }, { "sample": "Plasma", "range": "70 - 85 %", "average": "= 75.33" }, { "sample": "Serum", "range": "75 - 85 %", "average": "= 81.22" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab313516-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB313516-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human GIP Detection Antibody", "number":"AB313516-CMP09", "productcode":"" }, { "size":"1 x 200 µL", "name":"250X HRP-Streptavidin", "number":"AB313516-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human GIP Protein Standard", "number":"AB313516-CMP10", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB313516-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human GIP Antibody-coated ELISA Plate", "number":"AB313516-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB313516-CMP07", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB313516-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Glucose-dependent Insulinotropic Polypeptide (GIP) also known as gastric inhibitory polypeptide is a hormone with a molecular mass around 5 kDa. It is a 42-amino acid peptide secreted by the K cells in the duodenum and jejunum of the small intestine. This protein interacts with the GIP receptor which is found in various tissues including pancreatic beta cells adipose tissue and the central nervous system. Researchers utilize GIP ELISA for detecting and quantifying this peptide in research studies.
Biological function summary

The GIP plays a significant role in stimulating insulin secretion in response to glucose intake which does not function alone. It works as part of the enteroinsular axis influencing nutrient uptake and metabolism. GIP also regulates fat metabolism by acting on adipocytes therefore playing a part in energy homeostasis. The exploration of GIP activity often involves MSD assay development to better understand its biological implications.

Pathways

The glucose-stimulated insulin secretion pathway incorporates GIP to enhance the release of insulin from the pancreas. It interacts with other proteins such as insulin and glucagon-like peptide-1 (GLP-1) to exert its glucose-lowering effects. GIP's involvement in the incretin pathway supports the body's maintenance of energy balance and glucose homeostasis under normal physiological conditions.

Glucose homeostasis dysfunction links GIP to type 2 diabetes and obesity. Impairments in GIP secretion or receptor signaling are associated with these metabolic disorders. Studies suggest that dysregulation of GIP along with related proteins like insulin can contribute to disease progression. By targeting GIP researchers at places like Abcam are looking for potential therapies to tackle these health challenges.
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Product protocols

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Target data

See full target information C1QTNF1
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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