AB234571

Human GITR ELISA Kit

Recombinant
SimpleStep
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Human GITR ELISA Kit is a single-wash 90-min Simplestep used to quantify Human GITR with a sensitivity of 14 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD357, AITR, GITR, UNQ319/PRO364, TNFRSF18, Tumor necrosis factor receptor superfamily member 18, Activation-inducible TNFR family receptor, Glucocorticoid-induced TNFR-related protein

4 Images

Sandwich ELISA - Human GITR ELISA Kit (AB234571)

sELISA

Supplier Data

Sandwich ELISA - Human GITR ELISA Kit (AB234571)

Interpolated concentrations of spiked GITR in human serum, plasma and cell culture supernatant samples.

The concentrations of GITR were measured in duplicates, interpolated from the GITR standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (EDTA) 25%, plasma (citrate) 25%, plasma (heparin) 25% and cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Human GITR ELISA Kit (AB234571)

Interpolated concentrations of native GITR in human MCF7 cell extract based on a 1,000 μg/mL extract load.

The concentrations of GITR were measured in duplicate and interpolated from the GITR standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean GITR concentration was determined to be 757 pg/mL in MCF7 cell extract.

sELISA

Supplier Data

Sandwich ELISA - Human GITR ELISA Kit (AB234571)

Example of human GITR standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human GITR ELISA Kit (AB234571)

Example of human GITR standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 14 pg/mL

Range

78 - 5000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human GITR ELISA Kit (ab234571) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of GITR protein in cell culture extracts, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human GITR with 14 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "MCF7 Lysate", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.4" }, { "reproducibilityType": "Intra", "sample": "MCF7 Lysate", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.2" } ]

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Recovery

[ { "sample": "Serum", "range": "90 - 98 %", "average": "= 94" }, { "sample": "EDTA Plasma", "range": "90 - 96 %", "average": "= 93" }, { "sample": "Cell culture extracts", "range": "87 - 96 %", "average": "= 92" }, { "sample": "Heparin Plasma", "range": "92 - 101 %", "average": "= 95" }, { "sample": "Citrate plasma", "range": "86 - 91 %", "average": "= 89" }, { "sample": "Cell culture media", "range": "103 - 107 %", "average": "= 105" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GITR also known as TNFRSF18 is a 241 amino acid protein with a mass of approximately 27 kDa. It belongs to the tumor necrosis factor receptor superfamily. GITR is highly expressed on activated T cells and regulatory T cells (Tregs). The gene encoding GITR is located on chromosome 1 in humans. High surface expression is also observed in immune tissues such as the thymus spleen and lymph nodes playing an important role in immune responses.

Biological function summary

GITR acts as a costimulatory molecule that enhances T cell activation and proliferation. It does not function as part of a complex but interacts directly with ligand GITRL triggering downstream signaling cascades. This interaction is key in the modulation of immune responses notably in enhancing the activity of effector T cells and regulating Treg functions. Activation of GITR can reduce Treg-mediated suppression leading to enhanced immune responses.

Pathways

The mechanistic role of GITR involves NF-kB and MAPK signaling pathways. Through these GITR impacts immune responses by stimulating the production of cytokines and promoting cell survival and proliferation. GITR signaling intersects with pathways involving proteins such as NF-kB further integrating with the immune system's regulation crescendo.

GITR appears to play a role in autoimmune conditions like rheumatoid arthritis and potential cancer immunotherapy. In autoimmune diseases GITR modulates immune activity influencing the balance between inhibition and activation of T cells. In oncology GITR targeting aims to enhance immune responses against tumors. Through these conditions GITR shows connections to proteins such as CTLA-4 which also contributes to immune regulatory processes.

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Product protocols

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Target data

Receptor for TNFSF18. Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Mediated NF-kappa-B activation via the TRAF2/NIK pathway.

See full target information TNFRSF18

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human GITR ELISA Kit

Sandwich ELISA - Human GITR ELISA Kit (AB234571)

Sandwich ELISA - Human GITR ELISA Kit (AB234571)

Sandwich ELISA - Human GITR ELISA Kit (AB234571)

Sandwich ELISA - Human GITR ELISA Kit (AB234571)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Assay Kits

Assay Kits

Assay Kits

Primary Antibodies

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise