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AB309424

Human GLB1/Beta-galactosidase ELISA Kit

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Human GLB1/Beta-galactosidase ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human GLB1/Beta-galactosidase in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

ELNR1, GLB1, Beta-galactosidase, Acid beta-galactosidase, Elastin receptor 1, Lactase

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Sandwich ELISA - Human GLB1/Beta-galactosidase ELISA Kit (AB309424)
  • sELISA

Supplier Data

Sandwich ELISA - Human GLB1/Beta-galactosidase ELISA Kit (AB309424)

Example of standard curve using ab309424. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.45 ng/mL

Range

0.45 - 150 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human GLB1/Beta-galactosidase ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CGLB1/Beta-galactosidase in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human GLB1/Beta-galactosidase coated on a 96-well plate.
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Recovery

[ { "sample": "Serum", "range": "81 - 103 %", "average": "= 93.1" }, { "sample": "Plasma", "range": "96 - 108 %", "average": "= 102.5" }, { "sample": "Cell culture media", "range": "106 - 121 %", "average": "= 111.9" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab309424-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB309424-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB309424-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human GLB1/Beta-galactosidase Detection Antibody", "number":"AB309424-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human GLB1/Beta-galactosidase Antibody-coated ELISA Plate", "number":"AB309424-CMP08", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB309424-CMP06", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB309424-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human GLB1/Beta-galactosidase Protein Standard", "number":"AB309424-CMP10", "productcode":"" }, { "size":"1 x 200 µL", "name":"100X HRP-Streptavidin", "number":"AB309424-CMP11", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GLB1 also known as beta-galactosidase is an enzyme that catalyzes the hydrolysis of beta-galactosides into monosaccharides. The protein has a molecular mass of approximately 76 kDa. Researchers observe this enzyme expression in the lysosome where it functions to break down complex carbohydrates. Its activity depends on proper glycosylation and correct folding usually occurring within specific cellular compartments.
Biological function summary

GLB1 is integral to the degradation and recycling of glycoproteins and glycolipids in the cell. It forms part of the lysosomal enzyme complex responsible for processing macromolecules. Without its function the accumulation of substrates can occur affecting cellular metabolism. The enzyme ensures that metabolic waste is efficiently processed linking GLB1 to cellular homeostasis.

Pathways

Beta-galactosidase participates in the lysosomal storage and glycan metabolism pathways. It is important for the catabolism of glycoside forms of hormones and other biologically-active molecules. In these pathways GLB1 interacts with proteins such as hexosaminidase creating a network of lysosomal enzymes. These interactions enable coordinated degradation of glycoproteins maintaining balance within cellular systems.

GLB1 mutations associate with conditions like GM1 gangliosidosis and Morquio syndrome type B. These are lysosomal storage disorders resulting from the accumulation of unmetabolized substrates. In these diseases hexosaminidase and other lysosomal enzymes show relationships with GLB1 illuminating complex interactions within cellular pathology. Therapeutic research aims to correct or enhance enzyme function seeking to alleviate disorder symptoms.
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Product protocols

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Target data

Isoform 1. Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans.. Isoform 2. Has no beta-galactosidase catalytic activity, but plays functional roles in the formation of extracellular elastic fibers (elastogenesis) and in the development of connective tissue. Seems to be identical to the elastin-binding protein (EBP), a major component of the non-integrin cell surface receptor expressed on fibroblasts, smooth muscle cells, chondroblasts, leukocytes, and certain cancer cell types. In elastin producing cells, associates with tropoelastin intracellularly and functions as a recycling molecular chaperone which facilitates the secretions of tropoelastin and its assembly into elastic fibers.
See full target information GLB1
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websiteProtocolBooklet
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