Human GLi3 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human GLi3 in Human in Serum, Plasma, Cell culture supernatant samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Plasma, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 40 - 10000 pg/mL
- Sensitivity
- = 40 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Has a dual function as a transcriptional activator and a repressor of the sonic hedgehog (Shh) pathway, and plays a role in limb development. The full-length GLI3 form (GLI3FL) after phosphorylation and nuclear translocation, acts as an activator (GLI3A) while GLI3R, its C-terminally truncated form, acts as a repressor. A proper balance between the GLI3 activator and the repressor GLI3R, rather than the repressor gradient itself or the activator/repressor ratio gradient, specifies limb digit number and identity. In concert with TRPS1, plays a role in regulating the size of the zone of distal chondrocytes, in restricting the zone of PTHLH expression in distal cells and in activating chondrocyte proliferation. Binds to the minimal GLI-consensus sequence 5'-GGGTGGTC-3'.
Alternative names
Transcriptional activator GLI3, GLI3 form of 190 kDa, GLI3 full-length protein, GLI3-190, GLI3FL, GLI3
What's included?
Recommended products
Human GLi3 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human GLi3 in Human in Serum, Plasma, Cell culture supernatant samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Plasma, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 40 - 10000 pg/mL
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 40 pg/mL
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 81.93 | Range 68 - 100 % |
Sample type Plasma | Average % = 71.92 | Range 67 - 82 % |
Sample type Cell culture supernatant | Average % = 90.23 | Range 85 - 97 % |
Sample type Cell culture media | Average % = 90.23 | Range 85 - 97 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Human GLi3 ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human GLi3 in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human GLi3 coated on a 96-well plate.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Gli3 also known as GLI Family Zinc Finger 3 is a transcription factor that plays a significant role in the Hedgehog signaling pathway. Gli3 functions as both an activator and a repressor depending on its post-translational modification state. It has a molecular mass of approximately 172 kDa. Mainly expressed in the limb buds central nervous system and other embryonic tissues Gli3 influences the development of various structures during embryogenesis.
- Biological function summary
Gli3 is essential for proper organ and tissue development. It associates with other proteins including the Suppressor of Fused (SUFU) to form complexes that regulate target gene expression. Gli3 interacts with GLI1 and GLI2 all of which are part of the Gli family of transcription factors. These interactions modulate various developmental processes by controlling target gene transcription ensuring tissues develop correctly.
- Pathways
Gli3 is an important player in the Hedgehog signaling pathway and also impacts a secondary pathway the Wnt signaling pathway. Gli3 acts in coordination with the Sonic Hedgehog (SHH) protein regulating developmental gene expression. SHH binds to the Patched receptor affecting Gli3's function as a transcriptional repressor or activator. By these pathways Gli3 ensures proper cellular communication and structural formation.
- Associated diseases and disorders
Mutations or dysregulation of Gli3 are connected to two major conditions: Pallister-Hall Syndrome and Greig Cephalopolysyndactyly Syndrome. Both conditions involve limb malformations and other developmental defects due to abnormal GLI3 function. Furthermore Gli3 dysregulation links to abnormal activity of SUFU and GLI2 contributing to the pathogenesis of these syndromes. Understanding these interactions can provide critical insights into therapeutic strategies for related disorders.
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1 product image
Sandwich ELISA - Human GLi3 ELISA Kit (ab309444)
Example of standard curve using ab309444. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.
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Product protocols
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