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AB309444

Human GLi3 ELISA Kit

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Human GLi3 ELISA Kit is a Sandwich ELISA for the measurement of Human GLi3 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

Transcriptional activator GLI3, GLI3 form of 190 kDa, GLI3 full-length protein, GLI3-190, GLI3FL, GLI3

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Sandwich ELISA - Human GLi3 ELISA Kit (AB309444)
  • sELISA

Supplier Data

Sandwich ELISA - Human GLi3 ELISA Kit (AB309444)

Example of standard curve using ab309444. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 40 pg/mL

Range

40 - 10000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB307714

Anti-Gli3 antibody [EPR26837-58]

20ul selling size|RabMAb|Recombinant

Immunoprecipitation - Anti-Gli3 antibody [EPR26837-58] (AB307714)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human GLi3 ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human GLi3 in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human GLi3 coated on a 96-well plate.
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Recovery

[ { "sample": "Serum", "range": "68 - 100 %", "average": "= 81.93" }, { "sample": "Plasma", "range": "67 - 82 %", "average": "= 71.92" }, { "sample": "Cell culture supernatant", "range": "85 - 97 %", "average": "= 90.23" }, { "sample": "Cell culture media", "range": "85 - 97 %", "average": "= 90.23" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab309444-1x96Tests", "assetComponentDetails": [ { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB309444-CMP11", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human GLI-3 Protein Standard", "number":"AB309444-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human GLI-3 Antibody-coated ELISA Plate", "number":"AB309444-CMP08", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB309444-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human GLI-3 Detection Antibody", "number":"AB309444-CMP01", "productcode":"" }, { "size":"1 x 200 µL", "name":"150X HRP-Streptavidin", "number":"AB309444-CMP13", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B (Item E)", "number":"AB309444-CMP10", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent D (Item K)", "number":"AB309444-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB309444-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Gli3 also known as GLI Family Zinc Finger 3 is a transcription factor that plays a significant role in the Hedgehog signaling pathway. Gli3 functions as both an activator and a repressor depending on its post-translational modification state. It has a molecular mass of approximately 172 kDa. Mainly expressed in the limb buds central nervous system and other embryonic tissues Gli3 influences the development of various structures during embryogenesis.
Biological function summary

Gli3 is essential for proper organ and tissue development. It associates with other proteins including the Suppressor of Fused (SUFU) to form complexes that regulate target gene expression. Gli3 interacts with GLI1 and GLI2 all of which are part of the Gli family of transcription factors. These interactions modulate various developmental processes by controlling target gene transcription ensuring tissues develop correctly.

Pathways

Gli3 is an important player in the Hedgehog signaling pathway and also impacts a secondary pathway the Wnt signaling pathway. Gli3 acts in coordination with the Sonic Hedgehog (SHH) protein regulating developmental gene expression. SHH binds to the Patched receptor affecting Gli3's function as a transcriptional repressor or activator. By these pathways Gli3 ensures proper cellular communication and structural formation.

Mutations or dysregulation of Gli3 are connected to two major conditions: Pallister-Hall Syndrome and Greig Cephalopolysyndactyly Syndrome. Both conditions involve limb malformations and other developmental defects due to abnormal GLI3 function. Furthermore Gli3 dysregulation links to abnormal activity of SUFU and GLI2 contributing to the pathogenesis of these syndromes. Understanding these interactions can provide critical insights into therapeutic strategies for related disorders.
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Product protocols

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Target data

Has a dual function as a transcriptional activator and a repressor of the sonic hedgehog (Shh) pathway, and plays a role in limb development. The full-length GLI3 form (GLI3FL) after phosphorylation and nuclear translocation, acts as an activator (GLI3A) while GLI3R, its C-terminally truncated form, acts as a repressor. A proper balance between the GLI3 activator and the repressor GLI3R, rather than the repressor gradient itself or the activator/repressor ratio gradient, specifies limb digit number and identity. In concert with TRPS1, plays a role in regulating the size of the zone of distal chondrocytes, in restricting the zone of PTHLH expression in distal cells and in activating chondrocyte proliferation. Binds to the minimal GLI-consensus sequence 5'-GGGTGGTC-3'.
See full target information GLI3
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websiteProtocolBooklet
en
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Product promise

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