Human GLP1 (7-36) ELISA Kit ab184857 is a single-wash 90-min SimpleStep ELISA used to quantify human glucagon-like peptide-1 (GLP-1) (7-36) with a sensitivity of 25 pg/mL. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step.
- Colorimetric sandwich ELISA - 450nm readout; works on any plate standard plate reader
- Different formats for different needs: -384-well format available for high-throughput applications
- Broad sample compatibility – works with plasma, cell culture supernatants, and serum
- Cited in 10 publications
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Publications
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- International journal of molecular sciences 24:2023Isosinensetin Stimulates Glucagon-like Peptide-1 Secretion via Activation of hTAS2R50 and the G-Mediated Signaling Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSeung-Hyeon Lee,Hyun Min Ko,Wona Jee,Hyungsuk Kim,Won-Seok Chung,Hyeung-Jin JangPubMed 36835092
- Nature communications 13:63562022Functional and metabolic alterations of gut microbiota in children with new-onset type 1 diabetes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoxiao Yuan,Ruirui Wang,Bing Han,ChengJun Sun,Ruimin Chen,Haiyan Wei,Linqi Chen,Hongwei Du,Guimei Li,Yu Yang,Xiaojuan Chen,Lanwei Cui,Zhenran Xu,Junfen Fu,Jin Wu,Wei Gu,Zhihong Chen,Xin Fang,Hongxiu Yang,Zhe Su,Jing Wu,Qiuyue Li,Miaoying Zhang,Yufeng Zhou,Lei Zhang,Guang Ji,Feihong LuoPubMed 36289225
- Small (Weinheim an der Bergstrasse, Germany) 18:e22025662022Sunlight-Controllable Biopharmaceutical Production for Remote Emergency Supply of Directly Injectable Therapeutic Proteins.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBozhidar-Adrian Stefanov,Maysam Mansouri,Ghislaine Charpin-El Hamri,Martin FusseneggerPubMed 36084222
- ACS synthetic biology 10:3359-33682021Promoters with Consistent Expression throughout the Murine Gut.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJeremy Armetta,Michael Schantz-Klausen,Denis Shepelin,Ruben Vazquez-Uribe,Martin Iain Bahl,Martin Frederik Laursen,Tine Rask Licht,Morten O A SommerPubMed 34842418
- International journal of molecular sciences 22:2021NFAT5 Is Involved in GRP-Enhanced Secretion of GLP-1 by Sodium.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCaini Fan,Laureano D Asico,Van Anthony M Villar,Jessica Hunt,Santiago Cuevas,Ines Armando,Pedro A Jose,Prasad R KonkalmattPubMed 33921209
- Nutrients 12:2020The Metabolic Syndrome: Emerging Novel Insights Regarding the Relationship between the Homeostasis Model Assessment of Insulin Resistance and other Key Predictive Markers in Young Adults of Western Algeria.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMohammed Ilyes Belhayara et. al.PubMed 32164195
- Diabetes, metabolic syndrome and obesity : targets 12:2289-23022019Essential Role Of High Glucose-Induced Overexpression Of PKCβ And PKCδ In GLP-1 Resistance In Rodent Cardiomyocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXietian Pan et. al.PubMed 31807042
- Molecular medicine reports 18:2033-20422018Effects of isomaltulose on insulin resistance and metabolites in patients with non‑alcoholic fatty liver disease: A metabolomic analysis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTakumi Kawaguchi et. al.PubMed 29956790
- Therapeutics and clinical risk management 13:739-7502017Chitosan oligosaccharide improves the therapeutic efficacy of sitagliptin for the therapy of Chinese elderly patients with type 2 diabetes mellitus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLijie Zhao et. al.PubMed 28721055
- Molecular & cellular proteomics : MCP 13:3049-622014Progesterone receptor membrane component 1 is a functional part of the glucagon-like peptide-1 (GLP-1) receptor complex in pancreatic β cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMing Zhang et. al.PubMed 25044020
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 59.6 - 1000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 25 pg/mL
Abcam Recommends
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Glucagon. Plays a key role in glucose metabolism and homeostasis. Regulates blood glucose by increasing gluconeogenesis and decreasing glycolysis. A counterregulatory hormone of insulin, raises plasma glucose levels in response to insulin-induced hypoglycemia. Plays an important role in initiating and maintaining hyperglycemic conditions in diabetes. Glucagon-like peptide 1. Potent stimulator of glucose-dependent insulin release. Also stimulates insulin release in response to IL6 (PubMed:22037645). Plays important roles on gastric motility and the suppression of plasma glucagon levels. May be involved in the suppression of satiety and stimulation of glucose disposal in peripheral tissues, independent of the actions of insulin. Has growth-promoting activities on intestinal epithelium. May also regulate the hypothalamic pituitary axis (HPA) via effects on LH, TSH, CRH, oxytocin, and vasopressin secretion. Increases islet mass through stimulation of islet neogenesis and pancreatic beta cell proliferation. Inhibits beta cell apoptosis (Probable). Glucagon-like peptide 2. Stimulates intestinal growth and up-regulates villus height in the small intestine, concomitant with increased crypt cell proliferation and decreased enterocyte apoptosis. The gastrointestinal tract, from the stomach to the colon is the principal target for GLP-2 action. Plays a key role in nutrient homeostasis, enhancing nutrient assimilation through enhanced gastrointestinal function, as well as increasing nutrient disposal. Stimulates intestinal glucose transport and decreases mucosal permeability. Oxyntomodulin. Significantly reduces food intake. Inhibits gastric emptying in humans. Suppression of gastric emptying may lead to increased gastric distension, which may contribute to satiety by causing a sensation of fullness. Glicentin. May modulate gastric acid secretion and the gastro-pyloro-duodenal activity. May play an important role in intestinal mucosal growth in the early period of life.
Alternative names
Pro-glucagon, GCG
What's included?
Recommended products
Human GLP1 (7-36) ELISA Kit ab184857 is a single-wash 90-min SimpleStep ELISA used to quantify human glucagon-like peptide-1 (GLP-1) (7-36) with a sensitivity of 25 pg/mL. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step.
- Colorimetric sandwich ELISA - 450nm readout; works on any plate standard plate reader
- Different formats for different needs: -384-well format available for high-throughput applications
- Broad sample compatibility – works with plasma, cell culture supernatants, and serum
- Cited in 10 publications
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 59.6 - 1000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Microplate
- Sensitivity
- = 25 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Human Serum | n 8 | mean - | SD - | C.V. 7 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Human Serum | n 3 | mean - | SD - | C.V. 9 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 109 | Range 105 - 112 % |
Sample type Heparin Plasma | Average % = 110 | Range 104 - 112 % |
Sample type Citrate plasma | Average % = 115 | Range 107 - 123 % |
Sample type Cell culture media | Average % = 99 | Range 93 - 102 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human GLP1 (7-36) ELISA Kit ab184857 is a rapid single-wash 90-min ELISA kit to measure Human GLP1 (7-36) in cell culture supernatant, serum and plasma. This SimpleStep ELISA® sensitivity is 25 pg/mL.
How the assay works
Human GLP1 (7-36) SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
GLP-1(9-36) recombinant protein was diluted at 10ng/mL and assayed for cross reactivity. Signal was found at 7% from GLP-1 (7-36) protein assayed at the same concentration.
Human GLP1 (7-36) ELISA Kit ab184857 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm
How other researchers are using Human GLP1 (7-36) ELISA Kit ab184857
Human GLP1 (7-36) ELISA Kit ab184857 has been used to study isosinestin modulation of human glucagon like peptide 1 secretion as a therapy for diabetes mellitus(1) and gut microbial profiles and metabolic traits in type 1 diabetes(2).
References:
(1)Hyeung-Jin Jang et al. 2023, PMID: 36835092,(2)Feihong Luo et al. 2022, PMID: 36289225
Save your precious samples
Our 384-well Human GLP1 (7-36) ELISA Kit only requires a maxmum of 12.5µL
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation
- Human GLP1 (7-36) Antibody Pair - BSA and Azide free Human GLP1 (7-36) Antibody Pair - BSA and Azide free ab243974
- Anti-GLP1 (7-36) antibody [EPR4042-1] - BSA and Azide free (Capture) Anti-GLP1 (7-36) antibody [EPR4042-1] - BSA and Azide free (Capture) ab244627
- Anti-GLP1 (7-36) antibody [EPR4042-407] - BSA and Azide free (Detector) Anti-GLP1 (7-36) antibody [EPR4042-407] - BSA and Azide free (Detector) ab244887
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6 product images
Sandwich ELISA - Human GLP1 (7-36) ELISA Kit (ab184857)
Sample Data.
Ten individual healthy donors were evaluated for the presence of GLP-1 in serum using this assay. Serum of each donor was diluted 1/10 using sample diluent NS. The mean levels of GLP-1, after adjusting for dilution factor, were found at 433.5 pg/mL with a standard deviation of 277 pg/mL.
Sandwich ELISA - Human GLP1 (7-36) ELISA Kit (ab184857)
Example of GLP-1 standard curve.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human GLP1 (7-36) ELISA Kit (ab184857)
Example of GLP-1 standard curve. The GLP-1 standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human GLP1 (7-36) ELISA Kit (ab184857)
Ten individual healthy donors were evaluated for the presence of GLP-1 in serum using this assay.
Serum of each donor was diluted 1:10 using sample diluent NS. The mean levels of GLP-1, after adjusting for dilution factor, were found at 433.5 pg/mL with a standard deviation of 277 pg/mL.
Sandwich ELISA - Human GLP1 (7-36) ELISA Kit (ab184857)
Recombinant GLP-1 (7-36) was spiked into the following biological samples and diluted in a 2-fold dilution series in Sample Diluent NS.
Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.
Sandwich ELISA - Human GLP1 (7-36) ELISA Kit (ab184857)
Example of human GLP1 (7-36) standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
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