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AB325364

Human GLP1 (7-36) ELISA Kit, Chemiluminescent

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Human GLP1 (7-36) ELISA Kit, Chemiluminescent is a 55-min single-wash Simplestep IgniteTM ELISA for the rapid quantification of Human GLP1 (7-36) with higher sensitivity and broader measuring range.

- Single 30 min incubation and single -wash step for results in less than an hour
- Chemiluminescent detection allows a broad dynamic range :12.3 - 1000 pg/mL with a sensitivity of 8.6 pg/mL
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Patent Pending

View Alternative Names

Pro-glucagon, GCG

4 Images
Sandwich ELISA - Human GLP1 (7-36) ELISA Kit, Chemiluminescent (AB325364)
  • sELISA

Lab

Sandwich ELISA - Human GLP1 (7-36) ELISA Kit, Chemiluminescent (AB325364)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Human GLP1 (7-36) ELISA Kit, Chemiluminescent (AB325364)
  • sELISA

Lab

Sandwich ELISA - Human GLP1 (7-36) ELISA Kit, Chemiluminescent (AB325364)

Dynamic range comparison between the Chemiluminescent Human GLP1 (7-36) SimpleStep ELISA and colorimetric Human GLP1 (7-36) SimpleStep ELISA (ab184857).

Sandwich ELISA - Human GLP1 (7-36) ELISA Kit, Chemiluminescent (AB325364)
  • sELISA

Lab

Sandwich ELISA - Human GLP1 (7-36) ELISA Kit, Chemiluminescent (AB325364)

Standard curve comparison between the Chemiluminescent Human GLP1 (7-36) SimpleStep ELISA and colorimetric Human GLP1 (7-36) SimpleStep ELISA (ab184857) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human GLP1 (7-36) ELISA Kit, Chemiluminescent (AB325364)
  • sELISA

Lab

Sandwich ELISA - Human GLP1 (7-36) ELISA Kit, Chemiluminescent (AB325364)

Ten individual healthy donors were evaluated for the presence of GLP-1 in serum using this assay. Serum of each donor was diluted 1 : 10 using Sample Diluent NS. The mean levels of GLP-1, after adjusting for dilution factor, were found at 433.5 pg/mL with a standard deviation of 277 pg/mL.

Key facts

Detection method

Luminescent

Sample types

Serum, Citrate plasma, Heparin Plasma, Cell culture media

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 8.6 pg/mL

Range

12.3 - 1000 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human GLP1 (7-36) ELISA Kit, Chemiluminescent (ab325364) is a rapid single-wash sandwich 55-min ELISA to measure Human GLP1 (7-36) in Serum, Citrate Plasma, Heparin Plasma, Cell Culture Media with a sensitivity of 8.6 pg/ml.

How the assay works

Human GLP1 (7-36) ELISA Kit, Chemiluminescent (ab325364) SimpleStep ELISA®  employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time.  By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colormetric ELISAs.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply

Please refer to our protocol booklet for more details.

Human GLP1 (7-36) (ab325364) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature

2. Wash

3. Add prepared ChemiHRP development solution - incubate for 2 min

4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Human GLP1 (7-36) Antibody Pair - BSA and Azide free ab243974

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9" } ]
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Recovery

[ { "sample": "Serum", "range": "105 - 112 %", "average": "= 109" }, { "sample": "Citrate plasma", "range": "107 - 123 %", "average": "= 115" }, { "sample": "Heparin Plasma", "range": "104 - 112 %", "average": "= 110" }, { "sample": "Cell culture media", "range": "93 - 102 %", "average": "= 99" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Glucagon. Plays a key role in glucose metabolism and homeostasis. Regulates blood glucose by increasing gluconeogenesis and decreasing glycolysis. A counterregulatory hormone of insulin, raises plasma glucose levels in response to insulin-induced hypoglycemia. Plays an important role in initiating and maintaining hyperglycemic conditions in diabetes.. Glucagon-like peptide 1. Potent stimulator of glucose-dependent insulin release. Also stimulates insulin release in response to IL6 (PubMed : 22037645). Plays important roles on gastric motility and the suppression of plasma glucagon levels. May be involved in the suppression of satiety and stimulation of glucose disposal in peripheral tissues, independent of the actions of insulin. Has growth-promoting activities on intestinal epithelium. May also regulate the hypothalamic pituitary axis (HPA) via effects on LH, TSH, CRH, oxytocin, and vasopressin secretion. Increases islet mass through stimulation of islet neogenesis and pancreatic beta cell proliferation. Inhibits beta cell apoptosis (Probable).. Glucagon-like peptide 2. Stimulates intestinal growth and up-regulates villus height in the small intestine, concomitant with increased crypt cell proliferation and decreased enterocyte apoptosis. The gastrointestinal tract, from the stomach to the colon is the principal target for GLP-2 action. Plays a key role in nutrient homeostasis, enhancing nutrient assimilation through enhanced gastrointestinal function, as well as increasing nutrient disposal. Stimulates intestinal glucose transport and decreases mucosal permeability.. Oxyntomodulin. Significantly reduces food intake. Inhibits gastric emptying in humans. Suppression of gastric emptying may lead to increased gastric distension, which may contribute to satiety by causing a sensation of fullness.. Glicentin. May modulate gastric acid secretion and the gastro-pyloro-duodenal activity. May play an important role in intestinal mucosal growth in the early period of life.
See full target information GCG
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websiteProtocolBooklet
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Product promise

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