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AB288170

Human GLP2 ELISA Kit

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Human GLP2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human GLP2 with a sensitivity of 3.01 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
3 Images
Sandwich ELISA - Human GLP2 ELISA Kit (AB288170)
  • sELISA

Lab

Sandwich ELISA - Human GLP2 ELISA Kit (AB288170)

Interpolated concentration of native GLP2 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : serum 50%, plasma (citrate) 25%, plasma (EDTA) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human GLP2 ELISA Kit (AB288170)
  • sELISA

Lab

Sandwich ELISA - Human GLP2 ELISA Kit (AB288170)

Serum of eight individual healthy species male donors was measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean GLP2 concentration was determined to be 512 pg/mL with a range of 38 – 919 pg/mL.

Sandwich ELISA - Human GLP2 ELISA Kit (AB288170)
  • sELISA

Lab

Sandwich ELISA - Human GLP2 ELISA Kit (AB288170)

Example of Human GLP2 standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma, Heparin Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 3.01 pg/mL

Range

7.813 - 500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human GLP2 ELISA Kit (ab288170) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human GLP2 protein in serum, EDTA plasma, citrate plasma, heparin plasma and cell culture supernatant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human GLP2 with 3.01 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "0.9" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.05" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "102 - 111 %", "average": "= 107" }, { "sample": "Serum", "range": "89 - 93 %", "average": "= 91" }, { "sample": "EDTA Plasma", "range": "95 - 108 %", "average": "= 100" }, { "sample": "Citrate plasma", "range": "96 - 106 %", "average": "= 103" }, { "sample": "Heparin Plasma", "range": "103 - 113 %", "average": "= 108" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab288170-1x96Tests", "assetComponentDetails": [ { "size":"1 x 600 µL", "name":"Human GLP2 Lyophilized Recombinant Protein", "number":"AB288170-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human GLP2 Capture Antibody", "number":"AB288170-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"10X Human GLP2 Detector Antibody", "number":"AB288170-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB288170-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB288170-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB288170-CMP07", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB288170-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB288170-CMP06", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB288170-CMP11", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB288170-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glucagon-like peptide 2 (GLP2) also known as glucagon-like peptide-2 and GLP-2 is a peptide that is derived from the proglucagon gene and weighs approximately 4.2 kDa. It is expressed mostly in the intestinal mucosa particularly in the L-cells of the ileum and colon. GLP2 is a 33-amino-acid peptide hormone that is secreted in response to nutrient intake. This peptide is involved in various bodily functions acting mainly in the gastrointestinal tract.
Biological function summary

GLP2 functions to enhance intestinal growth and nutrient absorption. It promotes mucosal epithelial growth increases villus height and reduces gut permeability. These actions make GLP2 critical for maintaining the structural and functional integrity of the gastrointestinal tract. It operates independently and is not part of a larger protein complex but works alongside similar hormones for gut homeostasis.

Pathways

GLP2 is involved in the enteroendocrine signaling pathway. This includes the modulation of the GLP2 receptor which impacts intestinal growth and function. The peptide also shows interplay with proteins like glucagon-like peptide 1 (GLP1) as both affect energy balance and glucose homeostasis although through different mechanisms. GLP2's primary role in enhancing nutrient absorption complements GLP1's efforts in glucose regulation.

GLP2's link to short bowel syndrome and inflammatory bowel disease highlights its therapeutic potential. Its ability to improve intestinal adaptation and reduce inflammation positions GLP2 as a candidate for treating these conditions. Additionally a connection exists with glucagon-like peptide 1 (GLP1) due to their common origin and overlapping pathways although each has distinct functions and disease relevance.
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Product protocols

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Target data

See full target information GLP2
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websiteProtocolBooklet
en
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