AB315322

Human Glutathione Peroxidase 1 ELISA Kit

SimpleStep
What is this?

Be the first to review this product! Submit a review

(0 Publication)

Human Glutathione Peroxidase 1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Glutathione Peroxidase 1 with a sensitivity of 10 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Glutathione peroxidase 1, GPx-1, GSHPx-1, Cellular glutathione peroxidase, Phospholipid-hydroperoxide glutathione peroxidase GPX1, GPX1

3 Images

Sandwich ELISA - Human Glutathione Peroxidase 1 ELISA Kit (AB315322)

sELISA

Supplier Data

Sandwich ELISA - Human Glutathione Peroxidase 1 ELISA Kit (AB315322)

Example of human Glutathione Peroxidase 1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Glutathione Peroxidase 1 ELISA Kit (AB315322)

Standard curve comparison between the original Human Glutathione Peroxidase 1 SimpleStep ELISA (ab193767) and current Human Glutathione Peroxidase 1 SimpleStep ELISA (ab315322).

sELISA

Supplier Data

Sandwich ELISA - Human Glutathione Peroxidase 1 ELISA Kit (AB315322)

Interpolated concentration of native Glutathione Peroxidase 1 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : THP-1 cell extract 37.5 µg/mL and HeLa cell extract 25 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 10 pg/mL

Range

31.25 - 2000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

Product details

Human Glutathione Peroxidase 1 SimpleStep ELISA^® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Glutathione Peroxidase 1 protein in Cell Lysate. Quantitate Human Glutathione Peroxidase 1 with 10 pg/ml sensitivity.

SimpleStep ELISA^® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^® protocol summary in the image section for further details. Our SimpleStep ELISA^® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA^® microplate (ab315065) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA^® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style

left-column

Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 2.6" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.3" } ]

style

left-column

Recovery

[ { "sample": "Cell Lysate", "range": "111 - 119 %", "average": "= 116" } ]

style

left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab315322-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB315322-CMP09", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB315322-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB315322-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB315322-CMP08", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB315322-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Glutathione Peroxidase 1 Capture Antibody", "number":"AB315322-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Glutathione Peroxidase 1 Lyophilized Recombinant Protein", "number":"AB315322-CMP03", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB315322-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB315322-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Glutathione Peroxidase 1 Detector Antibody", "number":"AB315322-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB315322-CMP10", "productcode":"" } ] } } }

style

left-column

Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glutathione Peroxidase 1 (GPx1) also known as GSH peroxidase is a selenium-containing enzyme with a mass of approximately 22 kDa. It plays an important role in reducing hydrogen peroxide to water using glutathione as a substrate therefore protecting cells from oxidative damage. GPx1 is widely expressed in many tissues but is found in high concentrations in the liver and erythrocytes. Researchers use antibodies specific to GPx1 often obtained from specialized suppliers to study and quantify this enzyme in various biological samples.

Biological function summary

The enzyme guards cellular components by neutralizing free radicals. GPx1 is not part of a large complex but functions as a homotetramer. Its activity is essential for preserving the redox balance within the cell preventing cellular damage from reactive oxygen species (ROS). Enzyme-linked immunosorbent assays (ELISA) often measure GPx1 activity to assess oxidative stress levels in research and clinical settings.

Pathways

GPx1 is important in the glutathione metabolism and cellular antioxidant defense pathways. Within these pathways GPx1 works closely with proteins like glutathione reductase to maintain reduced glutathione levels. This interplay with other antioxidant proteins ensures cellular protection against oxidative damage. Studies focus on GPx1's role in these pathways as it underpins many critical cellular processes maintaining cell health.

Decreased GPx1 activity links to neurodegenerative diseases and cardiovascular disorders. In neurodegenerative diseases such as Alzheimer's the oxidative stress overwhelms the antioxidant defenses including GPx1. Similarly in cardiovascular disorders a diminished GPx1 activity correlates with increased oxidative stress contributing to disease progression. Researchers also investigate connections between GPx1 and other oxidative stress-related proteins like superoxide dismutase to fully understand GPx1's role in disease mechanisms.

style

left-column

Product protocols

style

left-column

Target data

Catalyzes the reduction of hydroperoxides in a glutathione-dependent manner thus regulating cellular redox homeostasis (PubMed : 11115402, PubMed : 36608588). Can reduce small soluble hydroperoxides such as H2O2, cumene hydroperoxide and tert-butyl hydroperoxide, as well as several fatty acid-derived hydroperoxides (PubMed : 11115402, PubMed : 36608588). In platelets catalyzes the reduction of 12-hydroperoxyeicosatetraenoic acid, the primary product of the arachidonate 12-lipoxygenase pathway (PubMed : 11115402).

See full target information Glutathione peroxidase 1

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human Glutathione Peroxidase 1 ELISA Kit

Sandwich ELISA - Human Glutathione Peroxidase 1 ELISA Kit (AB315322)

Sandwich ELISA - Human Glutathione Peroxidase 1 ELISA Kit (AB315322)

Sandwich ELISA - Human Glutathione Peroxidase 1 ELISA Kit (AB315322)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Assay Kits

Primary Antibodies

Proteins & Peptides

Primary Antibodies

Product promise