Human Glutathione Peroxidase 3/GPx-3 ELISA Kit- Intracellular is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Glutathione Peroxidase 3/GPx-3 - Intracellular in Cell Lysate, Tissue Extracts samples.
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate, Tissue Extracts
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 31.25 - 2000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 9.436 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione.
Alternative names
GPXP, GPX3, Glutathione peroxidase 3, GPx-3, GSHPx-3, Extracellular glutathione peroxidase, Plasma glutathione peroxidase, GPx-P, GSHPx-P
What's included?
Human Glutathione Peroxidase 3/GPx-3 ELISA Kit- Intracellular is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Glutathione Peroxidase 3/GPx-3 - Intracellular in Cell Lysate, Tissue Extracts samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate, Tissue Extracts
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 31.25 - 2000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 9.436 pg/mL
- General recovery
- 103%
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Extract | n 8 | mean - | SD - | C.V. 3.8 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Extract | n 3 | mean - | SD - | C.V. 8 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell Lysate | Average % = 103 | Range 98 - 111 % |
Sample type Tissue Extracts | Average % = 104 | Range 101 - 107 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human Glutathione Peroxidase 3/GPx-3 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Glutathione Peroxidase 3/GPx-3 protein in Cell Lysate, Tissue extracts. Quantitate Human Glutathione Peroxidase 3/GPx-3 with 9.436 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
- Also available in a fully validated 384-well format.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Sandwich ELISA - Human Glutathione Peroxidase 3/GPx-3 ELISA Kit- Intracellular (ab323520)
Interpolated concentrations of human Glutathione Peroxidase 3 / GPx-3 in liver tissue extract 96-well vs. 384-well plates.
Interpolated concentration of native Glutathione Peroxidase 3 / GPx-3 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 50 ?g/mL liver tissue extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer SSW.
Sandwich ELISA - Human Glutathione Peroxidase 3/GPx-3 ELISA Kit- Intracellular (ab323520)
Example of human Glutathione Peroxidase 3 / GPx-3 standard curve in 1X Cell Extraction Buffer SSW in 96-well vs. 384-well plate.
Example of human Glutathione Peroxidase 3 / GPx-3 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Glutathione Peroxidase 3/GPx-3 ELISA Kit- Intracellular (ab323520)
Interpolated concentrations of human Glutathione Peroxidase 3 / GPx-3 in kidney and liver tissue extracts.
Interpolated concentration of native Glutathione Peroxidase 3 / GPx-3 was measured in duplicate at different sample concentrations. Undiluted samples are as follows: 2.5 ?g/mL kidney tissue extract and 50 ?g/mL liver tissue extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer SSW.
Sandwich ELISA - Human Glutathione Peroxidase 3/GPx-3 ELISA Kit- Intracellular (ab323520)
Example of human Glutathione Peroxidase 3 / GPx-3 standard curve in 1X Cell Extraction Buffer SSW.
Example of human Glutathione Peroxidase 3 / GPx-3 standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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