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AB283966

Human Glutathione Synthetase ELISA Kit

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Human Glutathione Synthetase ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Glutathione Synthetase in Human in Biofluids samples.

View Alternative Names

Glutathione synthetase, GSH synthetase, GSH-S, Glutathione synthase, GSS

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Sandwich ELISA - Human Glutathione Synthetase ELISA Kit (AB283966)
  • sELISA

Supplier Data

Sandwich ELISA - Human Glutathione Synthetase ELISA Kit (AB283966)

Example of Human Glutathione Synthetase standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cerebral Spinal Fluid, Saliva, Urine, Plasma, Milk, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 74 pg/mL

Range

0.125 - 8 ng/mL

Assay time

4h

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Glutathione Synthetase ELISA Kit (ab283966) is designed for the detection of Glutathione Synthetase (GSS) in human plasma, serum, urine, saliva, milk, CSF, cell lysate and tissue samples.

This assay employs a quantitative sandwich enzyme immunoassay technique that measures human GSS in approximately 4 hours. A polyclonal antibody specific for human GSS has been pre-coated onto a 96-well microplate with removable strips. GSS in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human GSS, which is recognized by a streptavidin-peroxidase (SP) conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 60, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.5" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 60, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glutathione Synthetase (GSS) sometimes called GSS line is an enzyme that plays a central role in cellular function by catalyzing the ATP-dependent conversion of gamma-glutamylcysteine and glycine into glutathione. This enzyme has a molecular mass of about 70 kDa and exhibits high expression in the liver and red blood cells. It maintains the necessary cellular levels of glutathione a major antioxidant that protects cells from oxidative stress.
Biological function summary

GSS is responsible for the final step in glutathione biosynthesis. Acting independently GSS operates without forming a larger enzyme complex. Its enzymatic activity ensures adequate production of glutathione which regulates cellular redox homeostasis and detoxification processes. The availability of glutathione is critical for cellular respiration and immunity molecules essential for a well-functioning organism.

Pathways

The activity of GSS integrates into the gamma-glutamyl cycle and the detoxification pathway. It partners indirectly with enzymes like gamma-glutamylcysteine synthetase which precedes GSS in the glutathione biosynthesis pathway. This relationship positions GSS as an important element in sustaining normal cellular detoxification processes ensuring the neutralization of reactive oxygen species and maintaining the balance of free radicals.

Defects in GSS activity correlate with glutathione synthetase deficiency an autosomal recessive disorder that results in increased oxidative damage and metabolic acidosis. Symptoms range from hemolytic anemia to neurological complications due to impairments in oxidative stress management. The disorder links to proteins involved in antioxidant processes highlighting the importance of GSS in maintaining proper cellular function and disease prevention.
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Product protocols

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Target data

Catalyzes the production of glutathione from gamma-glutamylcysteine and glycine in an ATP-dependent manner (PubMed : 7646467, PubMed : 9215686). Glutathione (gamma-glutamylcysteinylglycine, GSH) is the most abundant intracellular thiol in living aerobic cells and is required for numerous processes including the protection of cells against oxidative damage, amino acid transport, the detoxification of foreign compounds, the maintenance of protein sulfhydryl groups in a reduced state and acts as a cofactor for a number of enzymes (PubMed : 10369661). Participates in ophthalmate biosynthesis in hepatocytes (By similarity).
See full target information Glutathione synthetase
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