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AB214031

Human Glycogen Synthase 1 ELISA Kit

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Human Glycogen Synthase 1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Glycogen Synthase 1 with a sensitivity of 14 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

GYS, GYS1, Glycogen synthase 1

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ELISA - Human Glycogen Synthase 1 ELISA Kit (AB214031)
  • ELISA

Supplier Data

ELISA - Human Glycogen Synthase 1 ELISA Kit (AB214031)
Sandwich ELISA - Human Glycogen Synthase 1 ELISA Kit (AB214031)
  • sELISA

Supplier Data

Sandwich ELISA - Human Glycogen Synthase 1 ELISA Kit (AB214031)

Example of Glycogen Synthase 1 standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Glycogen Synthase 1 ELISA Kit (AB214031)
  • sELISA

Supplier Data

Sandwich ELISA - Human Glycogen Synthase 1 ELISA Kit (AB214031)

Interpolated concentrations of native Glycogen Synthase 1 in mouse NIH3T3 extract and rat PC12 extract.

The concentrations of Glycogen Synthase 1 were measured in duplicate and interpolated from the Glycogen Synthase 1 standard curve and corrected for sample dilution and extract load. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Glycogen Synthase 1 concentration was determined to be 5.7 pg/μg NIH3T3 extract and 595 pg/μg PC12 extract

Sandwich ELISA - Human Glycogen Synthase 1 ELISA Kit (AB214031)
  • sELISA

Supplier Data

Sandwich ELISA - Human Glycogen Synthase 1 ELISA Kit (AB214031)

Interpolated concentrations of native Glycogen Synthase 1 in Human HELA, HEK293, and Liver Extract.

The concentrations of Glycogen Synthase 1 were measured in duplicate and interpolated from the Glycogen Synthase 1 standard curve and corrected for sample dilution and extract load. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Glycogen Synthase 1 concentration was determined to be 38 pg/μg HELA extract, 129 pg/μg HEK293 extract and 5.9 pg/μg Liver extract.

Sandwich ELISA - Human Glycogen Synthase 1 ELISA Kit (AB214031)
  • sELISA

Supplier Data

Sandwich ELISA - Human Glycogen Synthase 1 ELISA Kit (AB214031)

Example of Glycogen Synthase 1 standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture extracts, Tissue Extracts, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 14 pg/mL

Range

62.5 - 4000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Glycogen Synthase 1 ELISA Kit ab214031 is a rapid single-wash 90-min Sandwich ELISA to measure Human Glycogen Synthase 1 in cell culture extracts, plasma, serum, tissue extracts. This SimpleStep sensitivity is 14 pg/mL.

How the assay works

Human Glycogen Synthase 1 SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Glycogen Synthase 1 ELISA Kit ab214031 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Glycogen synthase 1/GYS1 antibody [EPR19261-142] - BSA and Azide free (Capture) ab242866
- Anti-Glycogen synthase 1/GYS1 antibody [EPR19261-36] - BSA and Azide free (Detector) ab242866

Glycogen Synthase 1 (also known as Glycogen [starch] synthase, muscle) is a glycosyltransferase enzyme involved in converting glucose to glycogen. Mutations in Glycogen Synthase 1 are associated with glycogen storage disease. Mouse and rat Glycogen Synthase 1 have 97% protein sequence identity to human Glycogen Synthase 1.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "HeLa Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.6" }, { "reproducibilityType": "Intra", "sample": "HeLa Extract", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.5" } ]
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Recovery

[ { "sample": "Serum", "range": "76 - 84 %", "average": "= 80" }, { "sample": "Tissue Extracts", "range": "105 - 112 %", "average": "= 108" }, { "sample": "Citrate plasma", "range": "85 - 92 %", "average": "= 89" }, { "sample": "EDTA Plasma", "range": "90 - 97 %", "average": "= 95" }, { "sample": "Heparin Plasma", "range": "77 - 83 %", "average": "= 81" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab214031-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB214031-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB214031-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB214031-CMP07", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB214031-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB214031-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB214031-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Glycogen Synthase 1 Capture Antibody", "number":"AB214031-CMP01", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB214031-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Glycogen Synthase 1 Lyophilized Recombinant Protein", "number":"AB214031-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Glycogen Synthase 1 Detector Antibody", "number":"AB214031-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB214031-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB214031-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glycogen synthase 1 (GYS1) also known as muscle glycogen synthase catalyzes the synthesis of glycogen from glucose. It adds glucose units from UDP-glucose to the non-reducing ends of glycogen chains facilitating glycogen elongation. The molecular mass of GYS1 is approximately 85 kDa. It is predominantly expressed in skeletal muscle tissue providing energy storage primarily in the form of glycogen. GYS1 is regulated through phosphorylation wherein it undergoes inactive to active conformational changes.
Biological function summary

This enzyme plays a critical role in the energy storage mechanism of muscle tissues. GYS1 functions as a part of the glycogen synthase complex interacting with various glycogen-associated proteins to modulate its activity. These interactions control the enzyme's affinity for its substrate and its susceptibility to regulatory signals like phosphorylation affecting glycogen synthesis efficiency. Through its activity GYS1 ensures a readily available reserve of glucose in muscle tissues for rapid energy production when required.

Pathways

Glycogen synthase 1 is a central component of the glycogen metabolism pathway. It acts alongside other enzymes such as glycogen phosphorylase which catalyzes glycogen breakdown. The balance between GYS1 and glycogen phosphorylase determines the rate of glycogen turnover within cells. GYS1 is further regulated by the insulin signaling pathway where insulin promotes glycogen synthesis by altering GYS1 activity alongside proteins like AKT influencing glucose homeostasis and energy management in tissues.

Dysregulation of GYS1 activity often associates with glycogen storage diseases notably Type 0 where deficiencies in glycogen storage lead to episodes of low blood sugar and reduced exercise capacity. Furthermore aberrations in GYS1 function contribute to insulin resistance and Type 2 diabetes conditions linked with impaired glucose metabolism. Altered GYS1 activity impacts proteins like glycogen phosphorylase affecting overall glycogen metabolism balance and contributing to metabolic disorders.
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Product protocols

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Target data

Glycogen synthase participates in the glycogen biosynthetic process along with glycogenin and glycogen branching enzyme. Extends the primer composed of a few glucose units formed by glycogenin by adding new glucose units to it. In this context, glycogen synthase transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan.
See full target information GYS1
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websiteProtocolBooklet
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