AB270217

Human Glypican 1 ELISA Kit (GPC1)

Recombinant
SimpleStep
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(1 Publication)

Human Glypican 1 ELISA Kit (GPC1) is a single-wash 90-min Simplestep used to quantify Human Glypican 1 (GPC1) with a sensitivity of 16.16 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Glypican-1, GPC1

5 Images

Sandwich ELISA - Human Glypican 1 ELISA Kit (GPC1) (AB270217)

sELISA

Supplier Data

Sandwich ELISA - Human Glypican 1 ELISA Kit (GPC1) (AB270217)

Interpolated concentrations of native Glypican 1 in human serum and plasma samples.

The concentrations of Glypican 1 were measured in duplicates, interpolated from the Glypican 1 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 25%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Glypican 1 concentration was determined to be 11.98 ng/mL in serum, 9.90 ng/mL in plasma (citrate) and 10.44 ng/mL in plasma (EDTA), and 9.93 ng/mL in plasma (Heparin).

sELISA

Supplier Data

Sandwich ELISA - Human Glypican 1 ELISA Kit (GPC1) (AB270217)

Interpolated concentrations of native Glypican 1 in human MCF7 extract based on a 500 μg/mL and placenta extract based on a 1,000 μg/mL extract load.

The concentrations of Glypican 1 were measured in duplicate and interpolated from the Glypican 1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Glypican 1 concentration was determined to be 4.48 ng/mL in MCF7 extract and 1.08 ng/mL in placenta.

sELISA

Supplier Data

Sandwich ELISA - Human Glypican 1 ELISA Kit (GPC1) (AB270217)

Interpolated concentrations of native Glypican 1 in human cell culture supernatant, and urine samples.

The concentrations of Glypican 1 were measured in duplicates, interpolated from the Glypican 1 standard curves and corrected for sample dilution. Undiluted samples are as follows : MCF7 supernatant 25%, HepG2 supernatant 50%, and urine 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Glypican 1 concentration was determined to be 10.14 ng/mL in MCF7 supernatant, 4.5 ng/mL in HepG2 supernatant and 7.87 ng/mL in urine.

sELISA

Supplier Data

Sandwich ELISA - Human Glypican 1 ELISA Kit (GPC1) (AB270217)

25% serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Glypican 1 concentration was determined to be 12.53 ng/mL with a range of 9.90 – 17.46 ng/mL.

sELISA

Supplier Data

Sandwich ELISA - Human Glypican 1 ELISA Kit (GPC1) (AB270217)

Example of human Glypican 1 standard curve in Sample Diluent NS.

The Glypican 1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Urine, Cell culture extracts, Tissue Extracts, Cell culture supernatant, Serum, Cell Lysate, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 16.16 pg/mL

Range

78.13 - 5000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Glypican 1 ELISA Kit (GPC1) (ab270217) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Glypican 1 (GPC1) protein in cell lysate, edta plasma, serum, urine, cell culture extracts, tissue extracts, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Glypican 1 (GPC1) with 16.16 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Human Glypican 1 (GPC1) is synthesized as a 558 amino acid (aa) precursor that contains a 23 aa signal sequence, a 507 aa mature segment, and a 28 aa C-terminal prosegment. The standard protein in this product is the mature form. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. GPC1 may play a role in the control of cell division and growth regulation. Human GPC1 shares 89%, 89%, and 87% sequence homology with mouse, rat, and bovine, respectively.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.2" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.8" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "110 - 117 %", "average": "= 114" }, { "sample": "Serum", "range": "106 - 117 %", "average": "= 112" }, { "sample": "EDTA Plasma", "range": "90 - 119 %", "average": "= 106" }, { "sample": "Cell culture extracts", "range": "83 - 100 %", "average": "= 87" }, { "sample": "Tissue Extracts", "range": "87 - 111 %", "average": "= 97" }, { "sample": "Citrate plasma", "range": "81 - 97 %", "average": "= 87" }, { "sample": "Heparin Plasma", "range": "96 - 101 %", "average": "= 99" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glypican 1 (GPC1) also known as glypican-1 is a heparan sulfate proteoglycan anchored to the cell membrane via a glycosylphosphatidylinositol (GPI) anchor. This protein belongs to the glypican family and has an approximate mass of 60 to 70 kDa. GPC1 is found mainly on the surface of cells in the central nervous system as well as in muscle tissues and certain epithelial cells. Its structure facilitates interaction with cell surface receptors and extracellular matrix components playing an important role in cellular communication and signaling processes.

Biological function summary

GPC1 influences the regulation of growth factors and morphogens and it has a significant role in the cell division and growth processes. It is not recognized as part of any larger protein complex but interacts with diverse ligand molecules. Researchers identify GPC1 as an important facilitator of fibroblast growth factors (FGF) and sonic hedgehog (Shh) signaling. Its interaction with molecular partners helps to regulate developmental signals important for proper cellular function and organ development.

Pathways

The contributions of GPC1 are observed in the hedgehog signaling pathway and fibroblast growth factor signaling. GPC1's interaction within the hedgehog pathway is critical for organizing cellular growth and differentiation while its involvement in the FGF pathway supports angiogenesis and wound healing. GPC1 coordinates with other glypican family members and related proteins like FGFR1 to mediate these signaling processes effectively.

Researchers have linked GPC1 with cancer progression and neurodegenerative diseases. Abnormal GPC1 expression is often noted in pancreatic cancer influencing tumor growth and metastasis through its modulation of growth factor signaling. Moreover its role in neurodegenerative conditions such as Alzheimer's disease establishes connections with amyloid-beta peptides and tau proteins contributing to disease pathogenesis. These associations make GPC1 an intriguing target for therapeutic intervention and a focus for ongoing medical research.

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Product protocols

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Target data

Cell surface proteoglycan that bears heparan sulfate. Binds, via the heparan sulfate side chains, alpha-4 (V) collagen and participates in Schwann cell myelination (By similarity). May act as a catalyst in increasing the rate of conversion of prion protein PRPN(C) to PRNP(Sc) via associating (via the heparan sulfate side chains) with both forms of PRPN, targeting them to lipid rafts and facilitating their interaction. Required for proper skeletal muscle differentiation by sequestering FGF2 in lipid rafts preventing its binding to receptors (FGFRs) and inhibiting the FGF-mediated signaling.

See full target information GPC1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of applied physiology (Bethesda, Md. : 1985) 137:1592-1603 PubMed39480270

2024

Impact of dietary supplementation of glycocalyx precursors on vascular function in type 2 diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

James A Smith,Francisco I Ramirez-Perez,Katherine Burr,Juan D Gonzalez-Vallejo,Mariana Morales-Quinones,Neil J McMillan,Larissa Ferreira-Santos,Neekun Sharma,Christopher A Foote,Luis A Martinez-Lemus,Jaume Padilla,Camila Manrique-Acevedo

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human Glypican 1 ELISA Kit (GPC1)

Sandwich ELISA - Human Glypican 1 ELISA Kit (GPC1) (AB270217)

Sandwich ELISA - Human Glypican 1 ELISA Kit (GPC1) (AB270217)

Sandwich ELISA - Human Glypican 1 ELISA Kit (GPC1) (AB270217)

Sandwich ELISA - Human Glypican 1 ELISA Kit (GPC1) (AB270217)

Sandwich ELISA - Human Glypican 1 ELISA Kit (GPC1) (AB270217)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Primary Antibodies

Assay Kits

Proteins & Peptides

Assay Kits

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise