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AB267640

Human Glypican 3 ELISA Kit

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Human Glypican 3 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Glypican 3 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

OCI5, GPC3, Glypican-3, GTR2-2, Intestinal protein OCI-5, MXR7

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Sandwich ELISA - Human Glypican 3 ELISA Kit (AB267640)
  • sELISA

Supplier Data

Sandwich ELISA - Human Glypican 3 ELISA Kit (AB267640)

Human Glypican 3 ELISA kit (ab267640) Standard curve.

Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture media, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.8 ng/mL

Range

0.8 - 600 ng/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Glypican 3 ELISA Kit is designed for the quantitative determination of Glypican 3 in cell culture supernatants, plasma and serum samples.

ΔNote: Human Glypican 3 concentration is low in normal serum/plasma and may not be detectable in this assay.

This assay employs an antibody specific for human Glypican 3 coated on a 96-well plate. Standards and samples are pipetted into the wells and Glypican 3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Glypican 3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Glypican 3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": "71 - 128 %", "average": "= 95" }, { "sample": "Plasma", "range": "69 - 85 %", "average": "= 75" }, { "sample": "Cell culture media", "range": "70 - 95 %", "average": "= 80" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab267640-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB267640-CMP06", "productcode":"" }, { "size":"1 x 200 µL", "name":"1200X HRP-Streptavidin Concentrate", "number":"AB267640-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB267640-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human Glypican 3 coated Microplate (12 x 8 wells)", "number":"AB267640-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB267640-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human Glypican 3 Detection Antibody", "number":"AB267640-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB267640-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Glypican 3 Standard (Lyophilized)", "number":"AB267640-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glypican 3 also known as GPC3 is a cell surface heparan sulfate proteoglycan with a core protein mass of approximately 70 kDa. This target is anchored to the external cell membrane through a glycosylphosphatidylinositol (GPI) anchor. Glypican 3 is expressed mainly in tissues such as the liver and kidneys and plays a role in modulating cell growth and development. In research studies it is often studied in various species including in the mouse and cynomolgus monkey models where it's referred to as mouse glypican 3 and cynomolgus glypican 3 respectively.
Biological function summary

Glypican 3 is involved in the modulation of signaling pathways that control cell proliferation and differentiation. It does not function as an isolated element; it has a role as part of a complex network that interacts with growth factors morphogens and other proteoglycans. Glypican 3 assists in the regulation of important biological events by influencing the accessibility and activity of signaling molecules on the cell surface. Its heparan sulfate chains interact with multiple proteins modifying their function and availability.

Pathways

Glypican 3 plays a significant part in the Wnt and Hedgehog signaling pathways. In the Wnt pathway it regulates the distribution and gradient formation of Wnt proteins which are essential for proper embryonic development. In the Hedgehog pathway glypican 3 modulates the distribution and activity of Hedgehog proteins important for tissue patterning and organogenesis. Other proteins closely related to glypican 3 in these pathways include those from the frizzled family in the Wnt pathway and Patched proteins in the Hedgehog pathway.

Glypican 3 has a strong connection to hepatocellular carcinoma (HCC) and Simpson-Golabi-Behmel syndrome (SGBS). In HCC increased expression of glypican 3 is often observed which associates it with tumor growth and proliferation. It functions alongside other oncogenic factors like alpha-fetoprotein (AFP) which is another marker for liver cancer. In SGBS mutations in the GPC3 gene result in defects in cellular signaling pathways leading to developmental abnormalities. This syndrome highlights the impact of glypican 3 on growth regulation and its interactions with insulin-like growth factors (IGFs).
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Product protocols

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Target data

Cell surface proteoglycan (PubMed : 14610063). Negatively regulates the hedgehog signaling pathway when attached via the GPI-anchor to the cell surface by competing with the hedgehog receptor PTC1 for binding to hedgehog proteins (By similarity). Binding to the hedgehog protein SHH triggers internalization of the complex by endocytosis and its subsequent lysosomal degradation (By similarity). Positively regulates the canonical Wnt signaling pathway by binding to the Wnt receptor Frizzled and stimulating the binding of the Frizzled receptor to Wnt ligands (PubMed : 16227623, PubMed : 24496449). Positively regulates the non-canonical Wnt signaling pathway (By similarity). Binds to CD81 which decreases the availability of free CD81 for binding to the transcriptional repressor HHEX, resulting in nuclear translocation of HHEX and transcriptional repression (By similarity). Inhibits the dipeptidyl peptidase activity of DPP4 (PubMed : 17549790). Plays a role in limb patterning and skeletal development by controlling the cellular response to BMP4 (By similarity). Modulates the effects of growth factors BMP2, BMP7 and FGF7 on renal branching morphogenesis (By similarity). Required for coronary vascular development (By similarity). Plays a role in regulating cell movements during gastrulation (By similarity).
See full target information GPC3
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websiteProtocolBooklet
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