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AB313967

Human Glypican 5 ELISA Kit

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Human Glypican 5 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Glypican 5 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

Glypican-5, GPC5

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Sandwich ELISA - Human Glypican 5 ELISA Kit (AB313967)
  • sELISA

Supplier Data

Sandwich ELISA - Human Glypican 5 ELISA Kit (AB313967)

Example of standard curve using ab313967. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 1.3 ng/mL

Range

1.3 - 300 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Glypican 5 ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Glypican 5 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human Glypican 5 coated on a 96-well plate.
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Recovery

[ { "sample": "Serum", "range": "98 - 110 %", "average": "= 103.6" }, { "sample": "Plasma", "range": "93 - 124 %", "average": "= 109" }, { "sample": "Cell culture media", "range": "114 - 135 %", "average": "= 120.4" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab313967-1x96Tests", "assetComponentDetails": [ { "size":"1 x 200 µL", "name":"500X HRP-Streptavidin", "number":"AB313967-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human Glypican 5 Antibody-coated ELISA Plate", "number":"AB313967-CMP09", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB313967-CMP07", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent D (Item K)", "number":"AB313967-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human Glypican 5 Detection Antibody", "number":"AB313967-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB313967-CMP08", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB313967-CMP04", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B (Item E)", "number":"AB313967-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human Glypican 5 Protein Standard", "number":"AB313967-CMP11", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glypican 5 (GPC5) is a cell surface heparan sulfate proteoglycan that is part of the glypican family. This protein weighs about 125 kDa. It is found mainly in the central nervous system and lung tissues. Glypicans including GPC5 are bound to the cell membrane via a glycosylphosphatidylinositol (GPI) anchor playing roles in cellular signaling at the cell surface.
Biological function summary

Glypican 5 modulates cell growth and differentiation by interacting with growth factors and morphogens acting as a coreceptor. It collaborates with heparan sulfate chains enabling the binding of signaling molecules at the cell surface. GPC5 does not work alone; it forms complexes with extracellular matrix components to influence cellular behavior. Its role extends to effect changes in the cellular microenvironment impacting development and tissue homeostasis.

Pathways

Glypican 5 serves as a part of two important pathways: the Wnt and Hedgehog signaling pathways. These pathways are critical for embryonic development and tissue patterning. GPC5 interacts with proteins like Frizzled and Patched which are receptors within these pathways. The interaction enhances signaling efficiency and contributes to the precise regulation of developmental processes.

Glypican 5 has links to cancers and congenital malformations. Abnormal expression of GPC5 has associations with lung cancer where it affects tumor growth and metastasis. Additionally GPC5 mutations or altered expression can lead to developmental disorders such as Simpson-Golabi-Behmel syndrome. In these conditions GPC5 interacts with proteins like Sonic Hedgehog complicating the disease mechanisms and influencing treatment strategies.
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Product protocols

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Target data

Cell surface proteoglycan that bears heparan sulfate.
See full target information Glypican-5
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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