Human GM-CSF ELISA Kit (CSF2) is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human GM-CSF (CSF2) in Cell culture media, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.
Colorimetric
Cell culture media, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
Sandwich (quantitative)
Human
15.625 - 1000 pg/mL
1h 30m
= 3.7 pg/mL
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Select an associated product type
Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
GMCSF, GMCSF, CSF2, Granulocyte-macrophage colony-stimulating factor, GM-CSF, Colony-stimulating factor, Molgramostin, Sargramostim, CSF
Human GM-CSF ELISA Kit (CSF2) is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human GM-CSF (CSF2) in Cell culture media, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.
Colorimetric
Cell culture media, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
Sandwich (quantitative)
Human
15.625 - 1000 pg/mL
1h 30m
Microplate
= 3.7 pg/mL
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Supernatant | n 8 | mean - | SD - | C.V. 1.4 |
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Supernatant | n 3 | mean - | SD - | C.V. 1.8 |
Sample type | Average % | Range |
---|---|---|
Sample type Cell culture supernatant | Average % = 100 | Range 99 - 102 % |
Sample type Serum | Average % = 81 | Range 78 - 86 % |
Sample type EDTA Plasma | Average % = 73 | Range 72 - 74 % |
Sample type Cell culture media | Average % = 100 | Range 98 - 101 % |
Sample type Heparin Plasma | Average % = 84 | Range 84 - 86 % |
Sample type Citrate plasma | Average % = 79 | Range 77 - 80 % |
Blue Ice
+4°C
+4°C
+4°C
Human GM-CSF ELISA (ab174448) kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of p53 protein in human serum, plasma and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate human GM-CSF with 3.7 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits.
Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. GM-CSF is used therapeutically in myeloid reconstitution following bone marrow transplant, bone marrow transplant engraftment failure or delay, mobilization and following transplantation of autologous peripheral blood progenitor cells, and following induction chemotherapy in older adults with acute myelogenous leukemia (AML). This kit recognizes Primate and Human GM-CSF which are 95% homologous.
This supplementary information is collated from multiple sources and compiled automatically.
GM-CSF also known as Granulocyte-Macrophage Colony-Stimulating Factor is a glycoprotein with a mass of approximately 23 kDa. This protein plays a mechanical role in stimulating the differentiation and proliferation of hematopoietic progenitor cells into granulocytes and macrophages. Its expression occurs in various cell types including macrophages T cells endothelial cells and fibroblasts. GM-CSF interacts with its specific receptor known as CSF2 receptor to exert its effects.
GM-CSF influences the immune system by enhancing the functional capacity of mature neutrophils macrophages and eosinophils. GM-CSF protein acts alone and not as part of a larger protein complex. It is important in regulating immune responses and inflammation. Due to its role in promoting the survival and activation of these immune cells GM-CSF is important for mounting an effective immune defense in response to infections and other challenges.
GM-CSF signaling is integral to the JAK-STAT pathway. This pathway is essential for transmitting signals from surface receptors like GM-CSF receptors into the nucleus thereby inducing gene expression that leads to cell survival and proliferation. GM-CSF also intersects with the ERK1/ERK2 MAPK pathway which is involved in regulating cellular processes such as division and differentiation. The GM-CSF receptor shares similarities with other cytokine receptors involved in these pathways making it related to various cytokines through its downstream signaling effects.
GM-CSF has a well-established connection to diseases like rheumatoid arthritis and multiple sclerosis. In rheumatoid arthritis overproduction of GM-CSF is linked to chronic inflammation and joint damage. In multiple sclerosis GM-CSF might contribute to the pathological immune responses against the central nervous system. Antibodies targeting GM-CSF or its receptor represent a therapeutic strategy for these autoimmune diseases. By modulating GM-CSF activity therapeutic approaches aim to reduce inflammation and autoimmunity highlighting the protein's importance in disease progression.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Example of human GM-CSF standard curve in Sample Diluent NS.
The GM-CSF standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Interpolated concentrations of spike GM-CSF in human serum, and plasma samples.
The concentrations of GM-CSF were measured in duplicates, interpolated from the GM-CSF standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Interpolated concentrations of native GM-CSF in human cell culture supernatant sample.
The concentrations of GM-CSF were measured in duplicates, interpolated from the GM-CSF standard curves and corrected for sample dilution. Undiluted samples are as follows: Stimulated PBMC Cell Culture Supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean GM-CSF concentration was determined to be 2599 pg/mL in Stimulated PBMC Cell Culture Supernatant and undetectable in unstimulated PBMC Cell Culture Supernatant.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com