AB174448

Human GM-CSF ELISA Kit (CSF2)

Recombinant
SimpleStep
What is this?

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(7 Publications)

Human GM-CSF ELISA Kit (CSF2) is a single-wash 90-min Simplestep used to quantify Human GM-CSF (CSF2) with a sensitivity of 3.7 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations

View Alternative Names

GMCSF, CSF2, Granulocyte-macrophage colony-stimulating factor, GM-CSF, Colony-stimulating factor, Molgramostin, Sargramostim, CSF

4 Images

ELISA - Human GM-CSF ELISA Kit (CSF2) (AB174448)

ELISA

Supplier Data

ELISA - Human GM-CSF ELISA Kit (CSF2) (AB174448)

sELISA

Unknown

Sandwich ELISA - Human GM-CSF ELISA Kit (CSF2) (AB174448)

Interpolated concentrations of native GM-CSF in human cell culture supernatant sample.

The concentrations of GM-CSF were measured in duplicates, interpolated from the GM-CSF standard curves and corrected for sample dilution. Undiluted samples are as follows : Stimulated PBMC Cell Culture Supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean GM-CSF concentration was determined to be 2599 pg/mL in Stimulated PBMC Cell Culture Supernatant and undetectable in unstimulated PBMC Cell Culture Supernatant.

sELISA

Unknown

Sandwich ELISA - Human GM-CSF ELISA Kit (CSF2) (AB174448)

Interpolated concentrations of spike GM-CSF in human serum, and plasma samples.

The concentrations of GM-CSF were measured in duplicates, interpolated from the GM-CSF standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

sELISA

Unknown

Sandwich ELISA - Human GM-CSF ELISA Kit (CSF2) (AB174448)

Example of human GM-CSF standard curve in Sample Diluent NS.

The GM-CSF standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 3.7 pg/mL

Range

15.625 - 1000 pg/mL

Assay time

1h 30m

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human GM-CSF ELISA Kit (CSF2) ab174448 is a rapid single-wash 90-min Sandwich ELISA to measure Human GM-CSF (CSF2)in cell culture media, cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 3.7 pg/mL.

How the assay works

Human GM-CSF (CSF2)SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human GM-CSF ELISA Kit (CSF2) ab174448 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. GM-CSF is used therapeutically in myeloid reconstitution following bone marrow transplant, bone marrow transplant engraftment failure or delay, mobilization and following transplantation of autologous peripheral blood progenitor cells, and following induction chemotherapy in older adults with acute myelogenous leukemia (AML). This kit recognizes Primate and Human GM-CSF which are 95% homologous.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.8" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.4" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "99 - 102 %", "average": "= 100" }, { "sample": "Serum", "range": "78 - 86 %", "average": "= 81" }, { "sample": "EDTA Plasma", "range": "72 - 74 %", "average": "= 73" }, { "sample": "Cell culture media", "range": "98 - 101 %", "average": "= 100" }, { "sample": "Heparin Plasma", "range": "84 - 86 %", "average": "= 84" }, { "sample": "Citrate plasma", "range": "77 - 80 %", "average": "= 79" } ]

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What's included?

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab174448-10x96Tests", "assetComponentDetails": [ { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB174448-CMP06" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB174448-CMP10" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB174448-CMP07" }, { "size":"2 x 250 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB174448-CMP04" }, { "size":"10 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB174448-CMP08" }, { "size":"1 x 6000 µL", "name":"10X Human GM-CSF Capture Antibody", "number":"AB174448-CMP01" }, { "size":"2 x 10 Vial", "name":"Human GM-CSF Lyophilized Recombinant Protein", "number":"AB174448-CMP03" }, { "size":"10 x 1 Unit", "name":"Plate Seals", "number":"AB174448-CMP09" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB174448-CMP05" }, { "size":"1 x 6000 µL", "name":"10X Human GM-CSF Detector Antibody", "number":"AB174448-CMP02" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab174448-1x96Tests", "assetComponentDetails": [ { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB174448-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB174448-CMP06", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB174448-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB174448-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB174448-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB174448-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human GM-CSF Lyophilized Recombinant Protein", "number":"AB174448-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human GM-CSF Capture Antibody", "number":"AB174448-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human GM-CSF Detector Antibody", "number":"AB174448-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB174448-CMP05", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GM-CSF also known as Granulocyte-Macrophage Colony-Stimulating Factor is a glycoprotein with a mass of approximately 23 kDa. This protein plays a mechanical role in stimulating the differentiation and proliferation of hematopoietic progenitor cells into granulocytes and macrophages. Its expression occurs in various cell types including macrophages T cells endothelial cells and fibroblasts. GM-CSF interacts with its specific receptor known as CSF2 receptor to exert its effects.

Biological function summary

GM-CSF influences the immune system by enhancing the functional capacity of mature neutrophils macrophages and eosinophils. GM-CSF protein acts alone and not as part of a larger protein complex. It is important in regulating immune responses and inflammation. Due to its role in promoting the survival and activation of these immune cells GM-CSF is important for mounting an effective immune defense in response to infections and other challenges.

Pathways

GM-CSF signaling is integral to the JAK-STAT pathway. This pathway is essential for transmitting signals from surface receptors like GM-CSF receptors into the nucleus thereby inducing gene expression that leads to cell survival and proliferation. GM-CSF also intersects with the ERK1/ERK2 MAPK pathway which is involved in regulating cellular processes such as division and differentiation. The GM-CSF receptor shares similarities with other cytokine receptors involved in these pathways making it related to various cytokines through its downstream signaling effects.

GM-CSF has a well-established connection to diseases like rheumatoid arthritis and multiple sclerosis. In rheumatoid arthritis overproduction of GM-CSF is linked to chronic inflammation and joint damage. In multiple sclerosis GM-CSF might contribute to the pathological immune responses against the central nervous system. Antibodies targeting GM-CSF or its receptor represent a therapeutic strategy for these autoimmune diseases. By modulating GM-CSF activity therapeutic approaches aim to reduce inflammation and autoimmunity highlighting the protein's importance in disease progression.

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Product protocols

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Target data

Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.

See full target information CSF2

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Aging 16:13201-13224 PubMed39560493

2024

Prostaglandin E regulates senescence and post-senescence neoplastic escape in primary human keratinocytes.

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Elise Srour,Nathalie Martin,Claire Drullion,Clémentine De Schutter,Joëlle Giroud,Adrien Pioger,Julie Deslé,Laure Saas,Joe Nassour,Julien Théry,Gauthier Decanter,Nicolas Penel,Chantal Vercamer,Clara Salazar-Cardozo,Corinne Abbadie,Olivier Pluquet

Nanoscale advances 5:5390-5398 PubMed37767034

2023

Thymoquinone-loaded lipid nanocapsules with promising anticancer activity for colorectal cancer.

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Species

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Mouna Selmi,Abir Salek,Mahassen Barboura,Leila Njim,Amine Trabelsi,Aida Lahmar,Nolwenn Lautram,Emilie Roger,Tarek Baati,Leila Chekir Ghedira

Proceedings of the National Academy of Sciences of the United States of America 120:e2213584120 PubMed36943879

2023

Influence of glycan structure on the colonization of on human respiratory epithelial cells.

Applications

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Ye-Yu Chun,Kai Sen Tan,Lisa Yu,Michelle Pang,Ming Hui Millie Wong,Rei Nakamoto,Wan-Zhen Chua,Amanda Huee-Ping Wong,Zhe Zhang Ryan Lew,Hsiao Hui Ong,Vincent T Chow,Thai Tran,De Yun Wang,Lok-To Sham

Immunity, inflammation and disease 10:e692 PubMed36301023

2022

HDAC4 depletion ameliorates IL-13-triggered inflammatory response and mucus production in nasal epithelial cells via activation of SIRT1/NF-κB signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Hangyu Xu,Lingjun Wang,Huaqun Chen,Hefei Cai

Cell reports 40:111153 PubMed35926468

2022

Cancer genes disfavoring T cell immunity identified via integrated systems approach.

Applications

Unspecified application

Species

Unspecified reactive species

Rigel J Kishton,Shashank J Patel,Amy E Decker,Suman K Vodnala,Maggie Cam,Tori N Yamamoto,Yogin Patel,Madhusudhanan Sukumar,Zhiya Yu,Michelle Ji,Amanda N Henning,Devikala Gurusamy,Douglas C Palmer,Roxana A Stefanescu,Andrew T Girvin,Winifred Lo,Anna Pasetto,Parisa Malekzadeh,Drew C Deniger,Kris C Wood,Neville E Sanjana,Nicholas P Restifo

Cell reports 35:109293 PubMed34192535

2021

Breast tumor stiffness instructs bone metastasis via maintenance of mechanical conditioning.

Applications

Unspecified application

Species

Unspecified reactive species

Adam W Watson,Adam D Grant,Sara S Parker,Samantha Hill,Michael B Whalen,Jayati Chakrabarti,Michael W Harman,Mackenzie R Roman,Brittany L Forte,Cody C Gowan,Raúl Castro-Portuguez,Lindsey K Stolze,Christian Franck,Darren A Cusanovich,Yana Zavros,Megha Padi,Casey E Romanoski,Ghassan Mouneimne

Nature communications 10:1780 PubMed30992428

2019

The role of platelets in mediating a response to human influenza infection.

Applications

Unspecified application

Species

Unspecified reactive species

Milka Koupenova,Heather A Corkrey,Olga Vitseva,Giorgia Manni,Catherine J Pang,Lauren Clancy,Chen Yao,Jeffrey Rade,Daniel Levy,Jennifer P Wang,Robert W Finberg,Evelyn A Kurt-Jones,Jane E Freedman

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human GM-CSF ELISA Kit (CSF2)

ELISA - Human GM-CSF ELISA Kit (CSF2) (AB174448)

Sandwich ELISA - Human GM-CSF ELISA Kit (CSF2) (AB174448)

Sandwich ELISA - Human GM-CSF ELISA Kit (CSF2) (AB174448)

Sandwich ELISA - Human GM-CSF ELISA Kit (CSF2) (AB174448)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

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