Skip to main content

Human GM-CSF ELISA Kit (CSF2) is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human GM-CSF (CSF2) in Cell culture media, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.

Be the first to review this product! Submit a review

Images

Sandwich ELISA - Human GM-CSF ELISA Kit (CSF2) (AB174448), expandable thumbnail
  • Sandwich ELISA - Human GM-CSF ELISA Kit (CSF2) (AB174448), expandable thumbnail
  • Sandwich ELISA - Human GM-CSF ELISA Kit (CSF2) (AB174448), expandable thumbnail

Publications

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

15.625 - 1000 pg/mL

Assay time

1h 30m

Sensitivity

= 3.7 pg/mL

Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

1 product for Alternative Version

Target data

Function

Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.

Alternative names

What's included?

10 x 96 Tests
Components
10X Human GM-CSF Capture Antibody
1 x 6000 µL
10X Human GM-CSF Detector Antibody
1 x 6000 µL
10X Wash Buffer PT (ab206977)
1 x 200 mL
Antibody Diluent 4BI
10 x 6 mL
Human GM-CSF Lyophilized Recombinant Protein
2 x 10 Vial
Plate Seals
10 x 1 Unit
Sample Diluent NS (ab193972)
2 x 250 mL
SimpleStep Pre-Coated 96-Well Microplate (ab206978)
10 x 1 Unit
Stop Solution
1 x 120 mL
TMB Development Solution
1 x 120 mL

Recommended products

Human GM-CSF ELISA Kit (CSF2) is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human GM-CSF (CSF2) in Cell culture media, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

15.625 - 1000 pg/mL

Assay time

1h 30m

Assay Platform

Microplate

Sensitivity

= 3.7 pg/mL

Precision

Intra assay

Sample

Supernatant

n

8

mean

-

SD

-

C.V.

1.4

Inter assay

Sample

Supernatant

n

3

mean

-

SD

-

C.V.

1.8

Recovery

Sample specific recovery

Sample type

Cell culture supernatant

Average %

= 100

Range

99 - 102 %

Sample type

Serum

Average %

= 81

Range

78 - 86 %

Sample type

EDTA Plasma

Average %

= 73

Range

72 - 74 %

Sample type

Cell culture media

Average %

= 100

Range

98 - 101 %

Sample type

Heparin Plasma

Average %

= 84

Range

84 - 86 %

Sample type

Citrate plasma

Average %

= 79

Range

77 - 80 %

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Human GM-CSF ELISA (ab174448) kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of p53 protein in human serum, plasma and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate human GM-CSF with 3.7 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits.

Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. GM-CSF is used therapeutically in myeloid reconstitution following bone marrow transplant, bone marrow transplant engraftment failure or delay, mobilization and following transplantation of autologous peripheral blood progenitor cells, and following induction chemotherapy in older adults with acute myelogenous leukemia (AML). This kit recognizes Primate and Human GM-CSF which are 95% homologous.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

GM-CSF also known as Granulocyte-Macrophage Colony-Stimulating Factor is a glycoprotein with a mass of approximately 23 kDa. This protein plays a mechanical role in stimulating the differentiation and proliferation of hematopoietic progenitor cells into granulocytes and macrophages. Its expression occurs in various cell types including macrophages T cells endothelial cells and fibroblasts. GM-CSF interacts with its specific receptor known as CSF2 receptor to exert its effects.

Biological function summary

GM-CSF influences the immune system by enhancing the functional capacity of mature neutrophils macrophages and eosinophils. GM-CSF protein acts alone and not as part of a larger protein complex. It is important in regulating immune responses and inflammation. Due to its role in promoting the survival and activation of these immune cells GM-CSF is important for mounting an effective immune defense in response to infections and other challenges.

Pathways

GM-CSF signaling is integral to the JAK-STAT pathway. This pathway is essential for transmitting signals from surface receptors like GM-CSF receptors into the nucleus thereby inducing gene expression that leads to cell survival and proliferation. GM-CSF also intersects with the ERK1/ERK2 MAPK pathway which is involved in regulating cellular processes such as division and differentiation. The GM-CSF receptor shares similarities with other cytokine receptors involved in these pathways making it related to various cytokines through its downstream signaling effects.

Associated diseases and disorders

GM-CSF has a well-established connection to diseases like rheumatoid arthritis and multiple sclerosis. In rheumatoid arthritis overproduction of GM-CSF is linked to chronic inflammation and joint damage. In multiple sclerosis GM-CSF might contribute to the pathological immune responses against the central nervous system. Antibodies targeting GM-CSF or its receptor represent a therapeutic strategy for these autoimmune diseases. By modulating GM-CSF activity therapeutic approaches aim to reduce inflammation and autoimmunity highlighting the protein's importance in disease progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Sandwich ELISA - Human GM-CSF ELISA Kit (CSF2) (ab174448), expandable thumbnail

    Sandwich ELISA - Human GM-CSF ELISA Kit (CSF2) (ab174448)

    Example of human GM-CSF standard curve in Sample Diluent NS.

    The GM-CSF standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • Sandwich ELISA - Human GM-CSF ELISA Kit (CSF2) (ab174448), expandable thumbnail

    Sandwich ELISA - Human GM-CSF ELISA Kit (CSF2) (ab174448)

    Interpolated concentrations of spike GM-CSF in human serum, and plasma samples.

    The concentrations of GM-CSF were measured in duplicates, interpolated from the GM-CSF standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

  • Sandwich ELISA - Human GM-CSF ELISA Kit (CSF2) (ab174448), expandable thumbnail

    Sandwich ELISA - Human GM-CSF ELISA Kit (CSF2) (ab174448)

    Interpolated concentrations of native GM-CSF in human cell culture supernatant sample.

    The concentrations of GM-CSF were measured in duplicates, interpolated from the GM-CSF standard curves and corrected for sample dilution. Undiluted samples are as follows: Stimulated PBMC Cell Culture Supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean GM-CSF concentration was determined to be 2599 pg/mL in Stimulated PBMC Cell Culture Supernatant and undetectable in unstimulated PBMC Cell Culture Supernatant.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com